Mapkapk3 mitogen-activated protein kinase-activated protein kinase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mapkapk3provided by MGI
Official Full Name: mitogen-activated protein kinase-activated protein kinase 3provided by MGI
Primary source: MGI:MGI:2143163
See related: Ensembl:ENSMUSG00000032577 AllianceGenome:MGI:2143163
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 3PK; MK3; MK-3; MAPKAP3; MAPKAP-K3; MapkKapk3
Summary: Predicted to enable calmodulin binding activity; mitogen-activated protein kinase binding activity; and protein serine/threonine kinase activity. Involved in macropinocytosis; response to lipopolysaccharide; and toll-like receptor signaling pathway. Predicted to be located in nucleoplasm. Predicted to be active in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; naris; skin; urinary system; and ventral grey horn. Human ortholog(s) of this gene implicated in patterned macular dystrophy 3. Orthologous to human MAPKAPK3 (MAPK activated protein kinase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in colon adult (RPKM 18.4), bladder adult (RPKM 12.5) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F1; 9 57.98 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (107132125..107167082, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (107254927..107289877, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

IL-33-activated murine mast cells control the dichotomy between RORgammat(+) and Helios(+) Tregs via the MK2/3-mediated IL-6 production in vitro
Title: IL-33-activated murine mast cells control the dichotomy between RORγt(+) and Helios(+) T(regs) via the MK2/3-mediated IL-6 production in vitro.
The MK2/3 are the only LPS-induced kinases for S52 and S178 of TTP and the role of MK2 and MK3 in the regulation of TNF biosynthesis is not restricted to phosphorylation of TTP at S52/S178 but includes independent processes, which could involve other TTP phosphorylations.
Title: The Role of TTP Phosphorylation in the Regulation of Inflammatory Cytokine Production by MK2/3.
this study shows that the loss of MK3 in mast cells decreases the IL-33-induced leukocyte recruitment and the resulting skin inflammation
Title: MK2/3 Are Pivotal for IL-33-Induced and Mast Cell-Dependent Leukocyte Recruitment and the Resulting Skin Inflammation.
A dominant mutation in MAPKAPK3 causes a new retinal dystrophy involving Bruch's membrane and retinal pigment epithelium.
Title: A dominant mutation in MAPKAPK3, an actor of p38 signaling pathway, causes a new retinal dystrophy involving Bruch's membrane and retinal pigment epithelium.
MK2/3 cascade plays a strategic role in controlling synaptic plasticity and cognition.
Title: The MK2/3 cascade regulates AMPAR trafficking and cognitive flexibility.
This study showed that cmpd28, a Mapkapk2/3 inhibitor, represents a potentially new approach to type 2 diabetes therapy.
Title: Treatment of Obese Insulin-Resistant Mice With an Allosteric MAPKAPK2/3 Inhibitor Lowers Blood Glucose and Improves Insulin Sensitivity.
Mk3-/- mice, challenged with IAV infection, show a strong shift toward an innate NK cell and adaptive Th1 immune response and a resistance to influenza.
Title: MAPKAP kinase 3 suppresses Ifng gene expression and attenuates NK cell cytotoxicity and Th1 CD4 T-cell development upon influenza A virus infection.
MAPKAPK-3 regulates SERCA2a expression and fiber type composition to modulate skeletal muscle and cardiomyocyte function.
Title: Mitogen-activated protein kinase-activated protein kinases 2 and 3 regulate SERCA2a expression and fiber type composition to modulate skeletal muscle and cardiomyocyte function.
Protein kinases MK2 and MK3 play critical roles in a mouse model of glomerulonephritis.
Title: Crucial roles of the protein kinases MK2 and MK3 in a mouse model of glomerulonephritis.
MK2 regulates LPS-induced IFNbeta expression and downstream STAT3 activation as it restrains MK3 from mediating negative regulatory effects
Title: Distinct functions of the mitogen-activated protein kinase-activated protein (MAPKAP) kinases MK2 and MK3: MK2 mediates lipopolysaccharide-induced signal transducers and activators of transcription 3 (STAT3) activation by preventing negative regulatory effects of MK3.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin-dependent protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables mitogen-activated protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in macropinocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-serine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein autophosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in response to cytokine	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to cytokine	ISO Inferred from Sequence Orthology more info
involved_in response to lipopolysaccharide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in toll-like receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in toll-like receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: MAP kinase-activated protein kinase 3

Names: MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAPK-3

NP_001303620.1

EC 2.7.11.1

NP_001408254.1

EC 2.7.11.1

NP_849238.1

EC 2.7.11.1

XP_006511680.1

EC 2.7.11.1

XP_006511681.1

EC 2.7.11.1

XP_006511682.1

EC 2.7.11.1

XP_036010401.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001316691.2 → NP_001303620.1 MAP kinase-activated protein kinase 3 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks two consecutive coding exons in the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. This variant is described in PMID:20570725.

Source sequence(s)

AL672070

Consensus CDS

CCDS85722.1

UniProtKB/TrEMBL

Q09HN3

Related

ENSMUSP00000141342.2, ENSMUST00000192054.2

Conserved Domains (2) summary

smart00220
Location:45 → 237

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl21453
Location:41 → 258

PKc_like; Protein Kinases, catalytic domain
NM_001421325.1 → NP_001408254.1 MAP kinase-activated protein kinase 3 isoform 3

Status: VALIDATED

Source sequence(s)

AL672070

UniProtKB/Swiss-Prot

B0QZU7, E9QNE1, Q3T9E6, Q3UMW7, Q8K0G3
NM_178907.4 → NP_849238.1 MAP kinase-activated protein kinase 3 isoform 1

See identical proteins and their annotated locations for NP_849238.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AL672070

Consensus CDS

CCDS23487.1

UniProtKB/Swiss-Prot

B0QZU7, E9QNE1, Q3T9E6, Q3UMW7, Q8K0G3

Related

ENSMUSP00000035194.3, ENSMUST00000035194.8

Conserved Domains (1) summary

cl21453
Location:41 → 306

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

107132125..107167082 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036154508.1 → XP_036010401.1 MAP kinase-activated protein kinase 3 isoform X4

UniProtKB/TrEMBL

Q09HN3

Conserved Domains (1) summary

cl21453
Location:41 → 288

PKc_like; Protein Kinases, catalytic domain
XM_006511618.5 → XP_006511681.1 MAP kinase-activated protein kinase 3 isoform X3

Conserved Domains (1) summary

cd14089
Location:10 → 244

STKc_MAPKAPK; Catalytic domain of the Serine/Threonine kinases, Mitogen-activated protein kinase-activated protein kinases
XM_006511619.3 → XP_006511682.1 MAP kinase-activated protein kinase 3 isoform X5

Conserved Domains (1) summary

cl21453
Location:1 → 197

PKc_like; Protein Kinases, catalytic domain
XM_006511617.5 → XP_006511680.1 MAP kinase-activated protein kinase 3 isoform X2

Conserved Domains (1) summary

cd14089
Location:20 → 249

STKc_MAPKAPK; Catalytic domain of the Serine/Threonine kinases, Mitogen-activated protein kinase-activated protein kinases