Dnajb1 DnaJ heat shock protein family (Hsp40) member B1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dnajb1provided by MGI
Official Full Name: DnaJ heat shock protein family (Hsp40) member B1provided by MGI
Primary source: MGI:MGI:1931874
See related: Ensembl:ENSMUSG00000005483 AllianceGenome:MGI:1931874
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DjB1; Hdj1; HSPF1; Hsp40; 0610007I11Rik
Summary: This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. The encoded protein may also inhibit apoptosis. Peritoneal macrophages derived from homozygous knockout mice for this gene exhibit impaired heat tolerance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
Expression: Ubiquitous expression in testis adult (RPKM 83.7), thymus adult (RPKM 27.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C2; 8 40.22 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (84334804..84339282)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (83608175..83612653)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Decreased miR-329-3p upregulates Adamts4 and Dnajb1 in mouse hepatic I/R injury in an age-independent manner.
Title: Decreased miR-329-3p upregulates Adamts4 and Dnajb1 in mouse hepatic I/R injury in an age-independent manner.
Study validates the DNAJB1-PRKACA fusion kinase as an oncogenic driver and candidate drug target for fibrolamellar hepatocellular carcinoma in mouse model in which tumorigenesis was significantly enhanced by genetic activation of beta-catenin.
Title: DNAJB1-PRKACA fusion kinase interacts with β-catenin and the liver regenerative response to drive fibrolamellar hepatocellular carcinoma.
Generation of the Dnajb1-Prkaca fusion gene in wild-type mice to be sufficient to initiate formation of tumors that have many features of human fibrolamellar hepatocellular carcinonma.
Title: CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates That the Dnajb1-Prkaca Gene Fusion Is Sufficient to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma.
Overexpression of DNAJB1 relieves TDP-43 mediated toxicity in primary rodent cortical neurons.
Title: Overexpression of the essential Sis1 chaperone reduces TDP-43 effects on toxicity and proteolysis.
a biochemical link between Hsp40 expression and Abeta(42) proteostasis that is cell specific
Title: Cell line specific modulation of extracellular aβ42 by Hsp40.
Hsc70, Hdj1 and Hdj2 interact with soluble and fibrillar alpha-synuclein
Title: Hsc70 protein interaction with soluble and fibrillar alpha-synuclein.
a role for Hsp40 crosstalk with CSPalpha in neuroprotection.
Title: Hsp40 couples with the CSPalpha chaperone complex upon induction of the heat shock response.
HSP-40 expression was detected after 2 h of heat stress
Title: Effect of thermal stress on early and late passaged mouse lens epithelial cells.
Hsp40, which was constitutively expressed in postmeiotic spermatogenic cells in testis, was integrated into spermatozoa as two components, that is, sperm head and tail of rodent spermatozoa.
Title: Identification of a heat-shock protein Hsp40, DjB1, as an acrosome- and a tail-associated component in rodent spermatozoa.
function in thermotolerance
Title: Heat shock protein 40/DjB1 is required for thermotolerance in early phase.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATPase activator activity	ISO Inferred from Sequence Orthology more info
enables ATPase binding	ISO Inferred from Sequence Orthology more info
enables Hsp70 protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables Hsp70 protein binding	ISO Inferred from Sequence Orthology more info
enables protein folding chaperone	IDA Inferred from Direct Assay more info	PubMed
enables protein-folding chaperone binding	ISO Inferred from Sequence Orthology more info
enables transcription corepressor activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription corepressor activity	ISO Inferred from Sequence Orthology more info
enables transcription regulator inhibitor activity	ISO Inferred from Sequence Orthology more info
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to heat	ISO Inferred from Sequence Orthology more info
involved_in chaperone cofactor-dependent protein refolding	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within chaperone cofactor-dependent protein refolding	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chaperone cofactor-dependent protein refolding	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of inclusion body assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ATP-dependent activity	ISO Inferred from Sequence Orthology more info
involved_in protein folding	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density	ISO Inferred from Sequence Orthology more info
located_in sperm head	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: dnaJ homolog subfamily B member 1

Names: DnaJ (Hsp40) homolog, subfamily B, member 1; heat shock 40 kDa protein 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001308227.1 → NP_001295156.1 dnaJ homolog subfamily B member 1 isoform 2

See identical proteins and their annotated locations for NP_001295156.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate 5' terminal exon, resulting in a distinct 5' UTR and the use of an in-frame downstream start site, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1.

Source sequence(s)

AA794374, AC151992, AK158530, BY476619, BY658568

Consensus CDS

CCDS85571.1

UniProtKB/TrEMBL

Q3TYL7

Related

ENSMUSP00000148685.2, ENSMUST00000212300.2

Conserved Domains (1) summary

cd10747
Location:62 → 226

DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40
NM_018808.3 → NP_061278.1 dnaJ homolog subfamily B member 1 isoform 1

See identical proteins and their annotated locations for NP_061278.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AA794374, AC151992, AK146657, BY251864, BY476619, BY658568

Consensus CDS

CCDS22456.1

UniProtKB/Swiss-Prot

Q9QYJ3

UniProtKB/TrEMBL

Q3TIT6, Q3TU79

Related

ENSMUSP00000005620.9, ENSMUST00000005620.10

Conserved Domains (3) summary

cd10747
Location:162 → 326

DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40

COG0484
Location:1 → 340

DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

pfam00226
Location:4 → 65

DnaJ; DnaJ domain