Nln neurolysin (metallopeptidase M3 family) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nlnprovided by MGI
Official Full Name: neurolysin (metallopeptidase M3 family)provided by MGI
Primary source: MGI:MGI:1923055
See related: Ensembl:ENSMUSG00000021710 AllianceGenome:MGI:1923055
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 4930472G13Rik
Summary: Enables peptidase activity and peptide binding activity. Acts upstream of or within regulation of gluconeogenesis and regulation of skeletal muscle fiber differentiation. Located in plasma membrane. Orthologous to human NLN (neurolysin). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E18 (RPKM 12.4), whole brain E14.5 (RPKM 7.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 D1; 13 56.16 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (104159558..104246170, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (104023050..104109673, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Peptidase neurolysin functions to preserve the brain after ischemic stroke in male mice.
Title: Peptidase neurolysin functions to preserve the brain after ischemic stroke in male mice.
These results confirm the identity of neurolysin as a novel Ang II binding site, suggesting that neurolysin may play a significant role in opposing the pathophysiological actions of the brain RAS and influencing brain morphology.
Title: Distribution of non-AT1, non-AT2 binding of 125I-sarcosine1, isoleucine8 angiotensin II in neurolysin knockout mouse brains.
These results suggest the exciting new possibility that Nln is a key enzyme for energy metabolism and could be a novel therapeutic target to improve glucose uptake and insulin sensitivity.
Title: Neurolysin knockout mice generation and initial phenotype characterization.
Neurolysin plays a role in modulating the brain's response to stroke and recovery after stroke.
Title: Functional up-regulation of endopeptidase neurolysin during post-acute and early recovery phases of experimental stroke in mouse brain.
novel angiotensin binding site
Title: Identification of membrane-bound variant of metalloendopeptidase neurolysin (EC 3.4.24.16) as the non-angiotensin type 1 (non-AT1), non-AT2 angiotensin binding site.
Melanoma cells secrete neurolysin which may have an important role in tumor proliferation/angiogenesis in vitro and in vivo.
Title: Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growth.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2)

General gene information

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Markers

RH126654 (e-PCR)
- UniSTS:208983

RH132947 (e-PCR)
- UniSTS:216230

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metalloendopeptidase activity	ISO Inferred from Sequence Orthology more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables oligopeptidase activity	ISO Inferred from Sequence Orthology more info
enables peptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables peptidase activity	ISO Inferred from Sequence Orthology more info
enables peptide binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in peptide catabolic process	ISO Inferred from Sequence Orthology more info
involved_in peptide metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of gluconeogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of skeletal muscle fiber differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial intermembrane space	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: neurolysin, mitochondrial

Names: MEP; microsomal endopeptidase; mitochondrial oligopeptidase M; neurotensin endopeptidase

NP_083723.1

EC 3.4.24.16

XP_011243001.1

EC 3.4.24.16

XP_011243002.1

EC 3.4.24.16

XP_011243003.1

EC 3.4.24.16

XP_030103312.1

EC 3.4.24.16

XP_036014106.1

EC 3.4.24.16

XP_036014107.1

EC 3.4.24.16

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_029447.2 → NP_083723.1 neurolysin, mitochondrial precursor

See identical proteins and their annotated locations for NP_083723.1

Status: PROVISIONAL

Source sequence(s)

AK079090

Consensus CDS

CCDS36772.1

UniProtKB/Swiss-Prot

Q3UJP1, Q8R3F4, Q91YP2

UniProtKB/TrEMBL

A0A286YD12

Related

ENSMUSP00000104938.4, ENSMUST00000109315.5

Conserved Domains (2) summary

cd06455
Location:60 → 699

M3A_TOP; Peptidase M3 Thimet oligopeptidase (TOP) also includes neurolysin

COG0339
Location:54 → 703

Dcp; Zn-dependent oligopeptidase [Posttranslational modification, protein turnover, chaperones]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

104159558..104246170 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011244699.3 → XP_011243001.1 neurolysin, mitochondrial isoform X1

UniProtKB/TrEMBL

A0A286YD12

Conserved Domains (1) summary

cd09605
Location:58 → 741

M3A; Peptidase M3A family includes thimet oligopeptidase, dipeptidyl carboxypeptidase and mitochondrial intermediate peptidase
XM_011244700.2 → XP_011243002.1 neurolysin, mitochondrial isoform X2

See identical proteins and their annotated locations for XP_011243002.1

UniProtKB/TrEMBL

A0A286YD12

Conserved Domains (1) summary

cd09605
Location:35 → 718

M3A; Peptidase M3A family includes thimet oligopeptidase, dipeptidyl carboxypeptidase and mitochondrial intermediate peptidase
XM_011244701.3 → XP_011243003.1 neurolysin, mitochondrial isoform X2

See identical proteins and their annotated locations for XP_011243003.1

UniProtKB/TrEMBL

A0A286YD12

Conserved Domains (1) summary

cd09605
Location:35 → 718

M3A; Peptidase M3A family includes thimet oligopeptidase, dipeptidyl carboxypeptidase and mitochondrial intermediate peptidase
XM_036158213.1 → XP_036014106.1 neurolysin, mitochondrial isoform X2

UniProtKB/TrEMBL

A0A286YD12

Conserved Domains (1) summary

cd09605
Location:35 → 718

M3A; Peptidase M3A family includes thimet oligopeptidase, dipeptidyl carboxypeptidase and mitochondrial intermediate peptidase
XM_036158214.1 → XP_036014107.1 neurolysin, mitochondrial isoform X3

UniProtKB/TrEMBL

A0A286YD12

Conserved Domains (1) summary

cd09605
Location:35 → 676

M3A; Peptidase M3A family includes thimet oligopeptidase, dipeptidyl carboxypeptidase and mitochondrial intermediate peptidase
XM_030247452.2 → XP_030103312.1 neurolysin, mitochondrial isoform X3

UniProtKB/TrEMBL

A0A286YD12

Conserved Domains (1) summary

cd09605
Location:35 → 676

M3A; Peptidase M3A family includes thimet oligopeptidase, dipeptidyl carboxypeptidase and mitochondrial intermediate peptidase