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Tssk4 testis-specific serine kinase 4 [ Mus musculus (house mouse) ]

Gene ID: 71099, updated on 5-Mar-2024

Summary

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Official Symbol
Tssk4provided by MGI
Official Full Name
testis-specific serine kinase 4provided by MGI
Primary source
MGI:MGI:1918349
See related
Ensembl:ENSMUSG00000007591 AllianceGenome:MGI:1918349
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
TSK-4; TSSK-4; 1700020B19Rik; 4933424F08Rik
Summary
Enables magnesium ion binding activity; protein homodimerization activity; and protein serine/threonine kinase activity. Involved in several processes, including flagellated sperm motility; positive regulation of CREB transcription factor activity; and protein phosphorylation. Located in sperm flagellum. Orthologous to human TSSK4 (testis specific serine kinase 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in testis adult (RPKM 29.3) and genital fat pad adult (RPKM 1.3) See more
Orthologs
human all
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Genomic context

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Location:
14 C3; 14 28.19 cM
Exon count:
5
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 14 NC_000080.7 (55880893..55889996)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 14 NC_000080.6 (55643651..55652539)

Chromosome 14 - NC_000080.7Genomic Context describing neighboring genes Neighboring gene REC8 meiotic recombination protein Neighboring gene importin 4 Neighboring gene transmembrane 9 superfamily member 1 Neighboring gene magnesium-dependent phosphatase 1 Neighboring gene neural precursor cell expressed, developmentally down-regulated gene 8

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Testis specific serine/threonine kinase 4 (Tssk4) maintains its kinase activity by phosphorylating itself at Thr-197. Wei Y, et al. Mol Biol Rep, 2013 Jan. PMID 23054012
  2. Isolation and characterization of mouse testis specific serine/threonine kinase 5 possessing four alternatively spliced variants. Wei Y, et al. J Biochem Mol Biol, 2007 Sep 30. PMID 17927909
  3. Testis-specific serine/threonine protein kinase 4 (Tssk4) phosphorylates Odf2 at Ser-76. Wang X, et al. Sci Rep, 2016 Mar 10. PMID 26961893, Free PMC Article
  4. Phosphorylated testis-specific serine/threonine kinase 4 may phosphorylate Crem at Ser-117. Fu G, et al. Biosci Biotechnol Biochem, 2016 Jun. PMID 26940607
  5. Tssk4 is essential for maintaining the structural integrity of sperm flagellum. Wang X, et al. Mol Hum Reprod, 2015 Feb. PMID 25361759

See all (18) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Testis-specific serine/threonine protein kinase 4 (Tssk4) phosphorylates Odf2 at Ser-76
    Title: Testis-specific serine/threonine protein kinase 4 (Tssk4) phosphorylates Odf2 at Ser-76.
  2. Phosphorylated Tssk4 might participate in testis genes expressions by phosphorylating Crem at Ser-117.
    Title: Phosphorylated testis-specific serine/threonine kinase 4 may phosphorylate Crem at Ser-117.
  3. findings reveal that Tssk4 is required for maintaining the structural integrity of sperm flagellum and male fertility.
    Title: Tssk4 is essential for maintaining the structural integrity of sperm flagellum.
  4. HSP90 function abolished the catalytic activities of TSSK4 and -6 but did not significantly alter the specific activities of TSSK1 and -2. Inhibition of HSP90 resulted in increased TSSK ubiquitination and proteasomal degradation
    Title: Heat shock protein 90 functions to stabilize and activate the testis-specific serine/threonine kinases, a family of kinases essential for male fertility.
  5. autophosphorylation at Thr-197 plays a critical role in maintaining Tssk4 kinase activity, which might be involved in spermiogenesis.
    Title: Testis specific serine/threonine kinase 4 (Tssk4) maintains its kinase activity by phosphorylating itself at Thr-197.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1)  1 citation
  • Endonuclease-mediated (2) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
NOT enables protein kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein serine/threonine kinase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein serine/threonine kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables protein-containing complex binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within cell differentiation IEA
Inferred from Electronic Annotation
more info
  
involved_in fertilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in flagellated sperm motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intracellular signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within multicellular organism development IEA
Inferred from Electronic Annotation
more info
  
involved_in peptidyl-serine phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in peptidyl-serine phosphorylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in peptidyl-threonine autophosphorylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of CREB transcription factor activity IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of CREB transcription factor activity ISO
Inferred from Sequence Orthology
more info
  
involved_in protein phosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein phosphorylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein phosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in spermatid development IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within spermatogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in acrosomal vesicle TAS
Traceable Author Statement
more info
 PubMed 
located_in cell projection IEA
Inferred from Electronic Annotation
more info
  
located_in cilium IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasmic vesicle IEA
Inferred from Electronic Annotation
more info
  
located_in motile cilium IDA
Inferred from Direct Assay
more info
 PubMed 
located_in sperm flagellum IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

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Preferred Names
testis-specific serine/threonine-protein kinase 4
Names
testis-specific kinase 4
testis-specific serine/threonine protein kinase 5 variant alpha
testis-specific serine/threonine protein kinase 5 variant beta
testis-specific serine/threonine protein kinase 5 variant delta
testis-specific serine/threonine protein kinase 5 variant gamma
NP_001240817.1
NP_001240818.1
NP_081949.1
XP_006519614.1
XP_006519615.1
XP_036014742.1
XP_036014743.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001253888.1 → NP_001240817.1  testis-specific serine/threonine-protein kinase 4 isoform 3

    See identical proteins and their annotated locations for NP_001240817.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a longer protein (isoform 3), compared to isoform 1.
    Source sequence(s)
    AC174678
    Consensus CDS
    CCDS88669.1
    UniProtKB/TrEMBL
    A0A2I3BQS0
    Related
    ENSMUSP00000154783.2, ENSMUST00000228395.2
    Conserved Domains (2) summary
    smart00220
    Location:25 → 303
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd14162
    Location:24 → 302
    STKc_TSSK4-like; Catalytic domain of testis-specific serine/threonine kinase 4 and similar proteins

  2. NM_001253889.1 → NP_001240818.1  testis-specific serine/threonine-protein kinase 4 isoform 4

    See identical proteins and their annotated locations for NP_001240818.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) uses two alternate in-frame splice sites in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1.
    Source sequence(s)
    AC174678, AK006144, AK016890
    Consensus CDS
    CCDS56959.1
    UniProtKB/TrEMBL
    A0A2I3BQS0
    Related
    ENSMUSP00000007735.9, ENSMUST00000007735.9
    Conserved Domains (2) summary
    smart00220
    Location:25 → 278
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cl21453
    Location:24 → 278
    PKc_like; Protein Kinases, catalytic domain

  3. NM_027673.3 → NP_081949.1  testis-specific serine/threonine-protein kinase 4 isoform 1

    See identical proteins and their annotated locations for NP_081949.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes isoform (1).
    Source sequence(s)
    AC174678, AK016890
    Consensus CDS
    CCDS88670.1
    UniProtKB/Swiss-Prot
    A3QQQ9, A3QQR0, A3QQR1, A9P6P7, A9P6P9, Q9D411, Q9DA58
    UniProtKB/TrEMBL
    A0A2I3BQE9
    Related
    ENSMUSP00000153752.2, ENSMUST00000226591.2
    Conserved Domains (2) summary
    smart00220
    Location:25 → 293
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd14162
    Location:24 → 292
    STKc_TSSK4-like; Catalytic domain of testis-specific serine/threonine kinase 4 and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000080.7 Reference GRCm39 C57BL/6J

    Range
    55880893..55889996
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036158850.1 → XP_036014743.1  testis-specific serine/threonine-protein kinase 4 isoform X4

    Conserved Domains (1) summary
    PHA03247
    Location:12 → 113
    PHA03247; large tegument protein UL36; Provisional

  2. XM_006519551.4 → XP_006519614.1  testis-specific serine/threonine-protein kinase 4 isoform X1

    Conserved Domains (1) summary
    cl21453
    Location:3 → 230
    PKc_like; Protein Kinases, catalytic domain

  3. XM_036158849.1 → XP_036014742.1  testis-specific serine/threonine-protein kinase 4 isoform X3

    UniProtKB/TrEMBL
    A0A2I3BQM6
    Conserved Domains (1) summary
    cl21453
    Location:24 → 149
    PKc_like; Protein Kinases, catalytic domain

  4. XM_006519552.1 → XP_006519615.1  testis-specific serine/threonine-protein kinase 4 isoform X2

    Conserved Domains (1) summary
    cl21453
    Location:46 → 191
    PKc_like; Protein Kinases, catalytic domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9D411.1 GenPept UniProtKB/Swiss-Prot:Q9D411

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