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LOC542375 opaque endosperm 2 [ Zea mays ]

Gene ID: 542375, updated on 14-Dec-2023

Summary

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Gene symbol
LOC542375
Gene description
opaque endosperm 2
Locus tag
ZEAMMB73_Zm00001d018971
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Zea mays
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea
Also known as
o2; Opaque-2; Opaque-e; GRMZM2G015534
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Genomic context

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See LOC542375 in Genome Data Viewer
Location:
chromosome: 7
Exon count:
7
Annotation release Status Assembly Chr Location
103 current Zm-B73-REFERENCE-NAM-5.0 (GCF_902167145.1) 7 NC_050102.1 (10951530..10954687)
102.20200423 previous assembly B73 RefGen_v4 (GCF_000005005.2) 7 NC_024465.2 (11075364..11078233)

Chromosome 7 - NC_050102.1Genomic Context describing neighboring genes Neighboring gene tryptophan synthase alpha Neighboring gene 40S ribosomal protein S23-like Neighboring gene uncharacterized LOC100193116 Neighboring gene IAA24 - auxin-responsive Aux/IAA family member

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize. Sethi M, et al. Sci Rep, 2021 Jan 28. PMID 33510248, Free PMC Article
  2. Molecular Mechanisms Underlying Increase in Lysine Content of Waxy Maize through the Introgression of the opaque2 Allele. Wang W, et al. Int J Mol Sci, 2019 Feb 5. PMID 30764507, Free PMC Article
  3. Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm. Zhan J, et al. Plant Cell, 2018 Oct. PMID 30262552, Free PMC Article
  4. Marker-assisted introgression of opaque2 allele for rapid conversion of elite hybrids into quality protein maize. Hossain F, et al. J Genet, 2018 Mar. PMID 29666347
  5. Introgression of opaque2 into Waxy Maize Causes Extensive Biochemical and Proteomic Changes in Endosperm. Zhou Z, et al. PLoS One, 2016. PMID 27391593, Free PMC Article

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The transcription factors ZmNAC128 and ZmNAC130 coordinate with Opaque2 to promote endosperm filling in maize.
    Title: The transcription factors ZmNAC128 and ZmNAC130 coordinate with Opaque2 to promote endosperm filling in maize.
  2. RNA polymerase common subunit ZmRPABC5b is transcriptionally activated by Opaque2 and essential for endosperm development in maize.
    Title: RNA polymerase common subunit ZmRPABC5b is transcriptionally activated by Opaque2 and essential for endosperm development in maize.
  3. Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize.
    Title: Expression profile of protein fractions in the developing kernel of normal, Opaque-2 and quality protein maize.
  4. A SnRK1-ZmRFWD3-Opaque2 Signaling Axis Regulates Diurnal Nitrogen Accumulation in Maize Seeds.
    Title: A SnRK1-ZmRFWD3-Opaque2 Signaling Axis Regulates Diurnal Nitrogen Accumulation in Maize Seeds.
  5. Opaque-2 role in regulation of endosperm cell differentiation and function.Opaque-2 and NKD2 coregulate a gene network associated with aleurone cell differentiation.
    Title: Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm.
  6. The opaque 2 gene regulated multiple metabolic pathways related to biological processes (BP) and molecular function (MP) during waxy maize endosperm development. In particular, in the o2o2wxwx lines, the two genes that encode the EF-1alpha and LHT1 were up-regulated, but the gene that encodes sulfur-rich proteins was down-regulated, raising the grain lysine content.
    Title: Molecular Mechanisms Underlying Increase in Lysine Content of Waxy Maize through the Introgression of the opaque2 Allele.
  7. recessive opaque2 (o2) allele that confers enhanced endosperm lysine and tryptophan, was introgressed using marker-assisted backcross breeding into three normal inbred lines
    Title: Marker-assisted introgression of opaque2 allele for rapid conversion of elite hybrids into quality protein maize.
  8. The protein encoded by this gene physically interacts with the MADS47 protein (Gene:542034).
    Title: ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2.
  9. o2 and Pbf RNA interference may affect the levels of some other transcription factor(s) or mRNA regulatory factor(s) that in turn would affect the transcript and protein levels of SSIIa and SBEI.
    Title: Maize endosperm-specific transcription factors O2 and PBF network the regulation of protein and starch synthesis.
  10. This study gives us an intriguing insight into the metabolism changes in endosperm of waxy maize introgressed with opaque2.
    Title: Introgression of opaque2 into Waxy Maize Causes Extensive Biochemical and Proteomic Changes in Endosperm.
Submit: New GeneRIF Correction See all GeneRIFs (22)

General gene information

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Markers

Homology

Gene Ontology Provided by RefSeq

Function Evidence Code Pubs
enables DNA-binding transcription factor activity IEA
Inferred from Electronic Annotation
more info
 PubMed 
Process Evidence Code Pubs
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
 PubMed 

General protein information

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Preferred Names
regulatory protein opaque-2
Names
opaque 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001111951.2 → NP_001105421.2  regulatory protein opaque-2

    See identical proteins and their annotated locations for NP_001105421.2

    Status: VALIDATED

    Source sequence(s)
    BT036731, CABHLF010000013
    UniProtKB/TrEMBL
    A0A804Q4M1, B4FHZ3
    Conserved Domains (2) summary
    cd14702
    Location:234 → 284
    bZIP_plant_GBF1; Basic leucine zipper (bZIP) domain of Plant G-box binding factor 1 (GBF1)-like transcription factors: a DNA-binding and dimerization domain
    pfam12498
    Location:299 → 414
    bZIP_C; Basic leucine-zipper C terminal

RefSeqs of Annotated Genomes: Zea mays Annotation Release 103 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference Zm-B73-REFERENCE-NAM-5.0 Primary Assembly

Genomic

  1. NC_050102.1 Reference Zm-B73-REFERENCE-NAM-5.0 Primary Assembly

    Range
    10951530..10954687
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_008653637.4 → XP_008651859.1  regulatory protein opaque-2 isoform X1

    See identical proteins and their annotated locations for XP_008651859.1

    UniProtKB/TrEMBL
    A0A1D6HTP7, B4FFY5
    Conserved Domains (2) summary
    cd14702
    Location:249 → 299
    bZIP_plant_GBF1; Basic leucine zipper (bZIP) domain of Plant G-box binding factor 1 (GBF1)-like transcription factors: a DNA-binding and dimerization domain
    pfam12498
    Location:314 → 429
    bZIP_C; Basic leucine-zipper C terminal

  2. XM_035960772.1 → XP_035816665.1  regulatory protein opaque-2 isoform X3

    UniProtKB/TrEMBL
    A0A804Q4M1, C0HIP4
    Conserved Domains (2) summary
    cd14702
    Location:228 → 278
    bZIP_plant_GBF1; Basic leucine zipper (bZIP) domain of Plant G-box binding factor 1 (GBF1)-like transcription factors: a DNA-binding and dimerization domain
    pfam12498
    Location:293 → 408
    bZIP_C; Basic leucine-zipper C terminal

  3. XM_035960770.1 → XP_035816663.1  regulatory protein opaque-2 isoform X2

    UniProtKB/TrEMBL
    A0A804Q4M1, B4FHZ3
    Conserved Domains (2) summary
    cd14702
    Location:234 → 284
    bZIP_plant_GBF1; Basic leucine zipper (bZIP) domain of Plant G-box binding factor 1 (GBF1)-like transcription factors: a DNA-binding and dimerization domain
    pfam12498
    Location:299 → 414
    bZIP_C; Basic leucine-zipper C terminal
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P12959.1 GenPept UniProtKB/Swiss-Prot:P12959

Gene LinkOut

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Molecular Biology Databases