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Ube2k ubiquitin-conjugating enzyme E2K [ Mus musculus (house mouse) ]

Gene ID: 53323, updated on 21-Apr-2024

Summary

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Official Symbol
Ube2kprovided by MGI
Official Full Name
ubiquitin-conjugating enzyme E2Kprovided by MGI
Primary source
MGI:MGI:1858216
See related
Ensembl:ENSMUSG00000029203 AllianceGenome:MGI:1858216
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Lig; Hip2; Hypg; HIP-2; E2-25k; D5Ertd601e
Summary
Predicted to enable ubiquitin conjugating enzyme activity; ubiquitin protein ligase binding activity; and ubiquitin-ubiquitin ligase activity. Acts upstream of or within regulation of proteasomal ubiquitin-dependent protein catabolic process. Located in cytoplasm; filopodium tip; and nucleus. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Orthologous to human UBE2K (ubiquitin conjugating enzyme E2 K). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in testis adult (RPKM 20.5), CNS E18 (RPKM 19.6) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
5 C3.1; 5 33.72 cM
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (65694588..65756332)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (65537245..65598989)

Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene VISTA enhancer mm1569 Neighboring gene STARR-seq mESC enhancer starr_13263 Neighboring gene STARR-positive B cell enhancer ABC_E4757 Neighboring gene UDP-glucose dehydrogenase Neighboring gene small integral membrane protein 14 Neighboring gene STARR-positive B cell enhancer ABC_E4758 Neighboring gene STARR-seq mESC enhancer starr_13264 Neighboring gene STARR-positive B cell enhancer ABC_E4759 Neighboring gene STARR-positive B cell enhancer ABC_E8059 Neighboring gene STARR-positive B cell enhancer ABC_E11290 Neighboring gene predicted gene, 40309 Neighboring gene PDS5 cohesin associated factor A Neighboring gene STARR-seq mESC enhancer starr_13267 Neighboring gene predicted gene, 24399 Neighboring gene STARR-positive B cell enhancer ABC_E3578 Neighboring gene STARR-seq mESC enhancer starr_13273 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:66156164-66156347 Neighboring gene predicted gene 5296 Neighboring gene NEDD4 binding protein 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. E2-25K SUMOylation inhibits proteasome for cell death during cerebral ischemia/reperfusion. Jeong EI, et al. Cell Death Dis, 2016 Dec 29. PMID 28032866, Free PMC Article
  2. Localization of huntingtin-interacting protein-2 (Hip-2) mRNA in the developing mouse brain. Tanno Y, et al. J Chem Neuroanat, 1999 Oct. PMID 10585161
  3. UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma. Fu W, et al. Int J Oncol, 2023 Apr. PMID 36896783, Free PMC Article
  4. Reduction of HIP2 expression causes motor function impairment and increased vulnerability to dopaminergic degeneration in Parkinson's disease models. Su J, et al. Cell Death Dis, 2018 Oct 3. PMID 30282965, Free PMC Article
  5. E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated Abeta neurotoxicity. Song S, et al. J Cell Biol, 2008 Aug 25. PMID 18710920, Free PMC Article

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma.
    Title: UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma.
  2. Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in Developing and Postnatal Kidneys of Dab1(-/-) (yotari) Mice.
    Title: Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in Developing and Postnatal Kidneys of Dab1(-/-) (yotari) Mice.
  3. HIP2 insufficiency as a contributing factor for PD.
    Title: Reduction of HIP2 expression causes motor function impairment and increased vulnerability to dopaminergic degeneration in Parkinson's disease models.
  4. E2-25K has a crucial role in oxidative stress and cerebral Ischemia/Reperfusion-induced damage through inhibiting proteasome via its SUMOylation.
    Title: E2-25K SUMOylation inhibits proteasome for cell death during cerebral ischemia/reperfusion.
  5. E2-25K/Hip-2 is thus an essential upstream regulator of the expression and activation of caspase-12 in endoplasmic reticulum stress-mediated Abeta neurotoxicity.
    Title: E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated Abeta neurotoxicity.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1)  1 citation
  • Endonuclease-mediated (6) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin conjugating enzyme activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin conjugating enzyme activity ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin conjugating enzyme activity TAS
Traceable Author Statement
more info
 PubMed 
enables ubiquitin protein ligase binding ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin-protein transferase activity ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin-ubiquitin ligase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in cellular response to interferon-beta ISO
Inferred from Sequence Orthology
more info
  
involved_in free ubiquitin chain polymerization ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of peptidyl-threonine phosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of tumor necrosis factor-mediated signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of type I interferon-mediated signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in protein K48-linked ubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein polyubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within regulation of proteasomal ubiquitin-dependent protein catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in filopodium tip IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
ubiquitin-conjugating enzyme E2 K
Names
E2 ubiquitin-conjugating enzyme K
huntingtin interacting protein 2
ubiquitin carrier protein
ubiquitin-conjugating enzyme E2-25 kDa
ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
ubiquitin-protein ligase
NP_001297547.1
NP_001297548.1
NP_058066.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001310618.1 → NP_001297547.1  ubiquitin-conjugating enzyme E2 K isoform 2

    See identical proteins and their annotated locations for NP_001297547.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks two alternate in-frame exons compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AK036068, AK139564, AK149463, CF538783
    Consensus CDS
    CCDS80289.1
    UniProtKB/TrEMBL
    A0A0J9YU07
    Related
    ENSMUSP00000143941.2, ENSMUST00000201984.4
    Conserved Domains (2) summary
    smart00212
    Location:22 → 103
    UBCc; Ubiquitin-conjugating enzyme E2, catalytic domain homologues
    cl21463
    Location:112 → 149
    UBA_like_SF; UBA domain-like superfamily

  2. NM_001310619.1 → NP_001297548.1  ubiquitin-conjugating enzyme E2 K isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks an exon in the central coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct, shorter N-terminus than isoform 1.
    Source sequence(s)
    AC112263, AC136513
    Conserved Domains (2) summary
    smart00212
    Location:2 → 84
    UBCc; Ubiquitin-conjugating enzyme E2, catalytic domain homologues
    cl21463
    Location:93 → 130
    UBA_like_SF; UBA domain-like superfamily

  3. NM_016786.4 → NP_058066.2  ubiquitin-conjugating enzyme E2 K isoform 1

    See identical proteins and their annotated locations for NP_058066.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AK036068, AK139564, AK149463
    Consensus CDS
    CCDS39098.1
    UniProtKB/Swiss-Prot
    P61087
    UniProtKB/TrEMBL
    Q3TL77, Q6ZWQ6
    Related
    ENSMUSP00000122471.2, ENSMUST00000142407.8
    Conserved Domains (2) summary
    cd00195
    Location:6 → 149
    UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
    cd14390
    Location:163 → 200
    UBA_II_E2_UBE2K; UBA domain of vertebrate ubiquitin-conjugating enzyme E2 K (UBE2K)

RNA

  1. NR_144566.1 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) lacks an alternate exon compared to variant 1. This variant is represented as non-coding because the predicted protein does not meet RefSeq quality criteria.
    Source sequence(s)
    AC112263, AC136513
    Related
    ENSMUST00000202679.4

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000071.7 Reference GRCm39 C57BL/6J

    Range
    65694588..65756332
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P61087.3 GenPept UniProtKB/Swiss-Prot:P61087

Gene LinkOut

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