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Ppp1r16b protein phosphatase 1, regulatory subunit 16B [ Mus musculus (house mouse) ]

Gene ID: 228852, updated on 5-Mar-2024

Summary

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Official Symbol
Ppp1r16bprovided by MGI
Official Full Name
protein phosphatase 1, regulatory subunit 16Bprovided by MGI
Primary source
MGI:MGI:2151841
See related
Ensembl:ENSMUSG00000037754 AllianceGenome:MGI:2151841
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Wdt4; TIMAP; ANKRD4; C130078N17Rik
Summary
Predicted to enable myosin phosphatase regulator activity and protein phosphatase 1 binding activity. Acts upstream of or within establishment of endothelial barrier. Predicted to be located in cell projection; nuclear speck; and perinuclear region of cytoplasm. Predicted to be active in plasma membrane. Is expressed in several structures, including brain; eye; heart; hemolymphoid system; and spinal cord. Orthologous to human PPP1R16B (protein phosphatase 1 regulatory subunit 16B). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in thymus adult (RPKM 16.6), lung adult (RPKM 14.9) and 17 other tissues See more
Orthologs
human all
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Genomic context

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Location:
2 H1; 2 78.72 cM
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (158508653..158608254)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (158666733..158766334)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene predicted gene 14204 Neighboring gene STARR-positive B cell enhancer ABC_E4515 Neighboring gene solute carrier family 32 (GABA vesicular transporter), member 1 Neighboring gene ARP5 actin-related protein 5 Neighboring gene STARR-positive B cell enhancer ABC_E10158 Neighboring gene microRNA 3474 Neighboring gene STARR-positive B cell enhancer ABC_E7879 Neighboring gene STARR-positive B cell enhancer mm9_chr2:158499319-158499620 Neighboring gene STARR-positive B cell enhancer ABC_E2650 Neighboring gene STARR-positive B cell enhancer ABC_E7880 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:158548828-158549026 Neighboring gene STARR-positive B cell enhancer ABC_E2056 Neighboring gene STARR-positive B cell enhancer ABC_E7881 Neighboring gene STARR-positive B cell enhancer ABC_E416 Neighboring gene family with sequence similarity 83, member D Neighboring gene STARR-seq mESC enhancer starr_06234 Neighboring gene DEAH-box helicase 35 Neighboring gene STARR-seq mESC enhancer starr_06235

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. mPPP1R16B is a novel mouse protein phosphatase 1 targeting subunit whose mRNA is located in cell bodies and dendrites of neurons in four distinct regions of the brain. Magdaleno S, et al. Brain Res Gene Expr Patterns, 2002 Oct. PMID 12638124
  2. TIMAP repression by TGFβ and HDAC3-associated Smad signaling regulates macrophage M2 phenotypic phagocytosis. Yang J, et al. J Mol Med (Berl), 2017 Mar. PMID 27709267
  3. TIMAP inhibits endothelial myosin light chain phosphatase by competing with MYPT1 for the catalytic protein phosphatase 1 subunit PP1cβ. Wang X, et al. J Biol Chem, 2019 Sep 6. PMID 31315927, Free PMC Article
  4. TIMAP protects endothelial barrier from LPS-induced vascular leakage and is down-regulated by LPS. Poirier C, et al. Respir Physiol Neurobiol, 2011 Dec 15. PMID 21907835, Free PMC Article
  5. TIMAP, a novel CAAX box protein regulated by TGF-beta1 and expressed in endothelial cells. Cao W, et al. Am J Physiol Cell Physiol, 2002 Jul. PMID 12055102

See all (23) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. TIMAP is suppressed by TGFbeta via HDAC3-associated Smad signaling.
    Title: TIMAP repression by TGFβ and HDAC3-associated Smad signaling regulates macrophage M2 phenotypic phagocytosis.
  2. These data confirmed the barrier-protective role of TIMAP
    Title: TIMAP protects endothelial barrier from LPS-induced vascular leakage and is down-regulated by LPS.
  3. mRNA is located in cell bodies and dendrites of neurons in four distinct regions of the brain.
    Title: mPPP1R16B is a novel mouse protein phosphatase 1 targeting subunit whose mRNA is located in cell bodies and dendrites of neurons in four distinct regions of the brain.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1)  1 citation
  • Endonuclease-mediated (1) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables enzyme inhibitor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables myosin phosphatase regulator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein phosphatase regulator activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in establishment of endothelial barrier IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within establishment of endothelial barrier IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in establishment of endothelial barrier ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of peptidyl-serine dephosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of protein dephosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of endothelial cell proliferation ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of protein dephosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of protein dephosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of sprouting angiogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cell projection ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in nuclear speck ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
located_in perinuclear region of cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
protein phosphatase 1 regulatory inhibitor subunit 16B
Names
CAAX box protein TIMAP
TGF-beta-inhibited membrane-associated protein
protein phosphatase 1, regulatory (inhibitor) subunit 16B
whn-dependent transcript 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001159662.1 → NP_001153134.1  protein phosphatase 1 regulatory inhibitor subunit 16B

    See identical proteins and their annotated locations for NP_001153134.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same protein.
    Source sequence(s)
    AK037627, AL663077, BY718617
    Consensus CDS
    CCDS16991.1
    UniProtKB/Swiss-Prot
    Q8VHQ3
    UniProtKB/TrEMBL
    A2AC90, Q544H9
    Related
    ENSMUSP00000039540.5, ENSMUST00000045503.11
    Conserved Domains (3) summary
    PHA03095
    Location:71 → 301
    PHA03095; ankyrin-like protein; Provisional
    sd00045
    Location:71 → 98
    ANK; ANK repeat [structural motif]
    pfam12796
    Location:72 → 164
    Ank_2; Ankyrin repeats (3 copies)

  2. NM_153089.4 → NP_694729.1  protein phosphatase 1 regulatory inhibitor subunit 16B

    See identical proteins and their annotated locations for NP_694729.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein.
    Source sequence(s)
    AK037627, AL663077
    Consensus CDS
    CCDS16991.1
    UniProtKB/Swiss-Prot
    Q8VHQ3
    UniProtKB/TrEMBL
    A2AC90, Q544H9
    Related
    ENSMUSP00000099405.4, ENSMUST00000103116.10
    Conserved Domains (3) summary
    PHA03095
    Location:71 → 301
    PHA03095; ankyrin-like protein; Provisional
    sd00045
    Location:71 → 98
    ANK; ANK repeat [structural motif]
    pfam12796
    Location:72 → 164
    Ank_2; Ankyrin repeats (3 copies)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    158508653..158608254
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011239493.3 → XP_011237795.1  protein phosphatase 1 regulatory inhibitor subunit 16B isoform X2

    Conserved Domains (3) summary
    PHA03095
    Location:71 → 301
    PHA03095; ankyrin-like protein; Provisional
    sd00045
    Location:71 → 98
    ANK; ANK repeat [structural motif]
    pfam12796
    Location:72 → 164
    Ank_2; Ankyrin repeats (3 copies)

  2. XM_011239492.4 → XP_011237794.1  protein phosphatase 1 regulatory inhibitor subunit 16B isoform X1

    See identical proteins and their annotated locations for XP_011237794.1

    UniProtKB/Swiss-Prot
    Q8VHQ3
    UniProtKB/TrEMBL
    A2AC90, Q544H9
    Related
    ENSMUSP00000117310.2, ENSMUST00000145073.2
    Conserved Domains (3) summary
    PHA03095
    Location:71 → 301
    PHA03095; ankyrin-like protein; Provisional
    sd00045
    Location:71 → 98
    ANK; ANK repeat [structural motif]
    pfam12796
    Location:72 → 164
    Ank_2; Ankyrin repeats (3 copies)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8VHQ3.1 GenPept UniProtKB/Swiss-Prot:Q8VHQ3

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