Lpcat1 lysophosphatidylcholine acyltransferase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Lpcat1provided by MGI
Official Full Name: lysophosphatidylcholine acyltransferase 1provided by MGI
Primary source: MGI:MGI:2384812
See related: Ensembl:ENSMUSG00000021608 AllianceGenome:MGI:2384812
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: rd11; Aytl2; LPCAT; LPCAT-1; mLPCAT1; lysoPAFAT; 2900035H07Rik
Summary: Enables O-acyltransferase activity. Involved in phospholipid biosynthetic process. Acts upstream of or within several processes, including negative regulation of phosphatidylcholine biosynthetic process; positive regulation of protein catabolic process; and surfactant homeostasis. Located in Golgi apparatus; endoplasmic reticulum; and membrane. Is expressed in several structures, including eye; genitourinary system; lung; nervous system; and vascular system. Orthologous to human LPCAT1 (lysophosphatidylcholine acyltransferase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in lung adult (RPKM 143.7), liver E14.5 (RPKM 30.7) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 C1; 13 39.97 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (73615332..73664539)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (73467231..73516420)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lysophosphatidylcholine acyltransferase 1 alleviates silica-induced pulmonary fibrosis by modulating lipid metabolism.
Title: Lysophosphatidylcholine acyltransferase 1 alleviates silica-induced pulmonary fibrosis by modulating lipid metabolism.
Lysophosphatidylcholine acyltransferase 1 controls mitochondrial reactive oxygen species generation and survival of retinal photoreceptor cells.
Title: Lysophosphatidylcholine acyltransferase 1 controls mitochondrial reactive oxygen species generation and survival of retinal photoreceptor cells.
ROBO4 deletion ameliorates PAF-mediated skin inflammation via regulating the mRNA translation efficiency of LPCAT1/LPCAT2 and the expression of PAF receptor.
Title: ROBO4 deletion ameliorates PAF-mediated skin inflammation via regulating the mRNA translation efficiency of LPCAT1/LPCAT2 and the expression of PAF receptor.
These results provide insight into understanding of the retinal degeneration seen in rd11 mice that lack LPCAT1.
Title: Lysophosphatidylcholine acyltransferase 1 protects against cytotoxicity induced by polyunsaturated fatty acids.
LPCAT1 interacts with StarD10 and that amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction.
Title: Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) Specifically Interacts with Phospholipid Transfer Protein StarD10 to Facilitate Surfactant Phospholipid Trafficking in Alveolar Type II Cells.
Almost wild-type LPCAT1 expression in photoreceptors with strong rod rhodopsin and M/S cone opsin staining, and normal visually-guided water maze behavioral performances were observed in treated rd11 mice.
Title: AAV-mediated lysophosphatidylcholine acyltransferase 1 (Lpcat1) gene replacement therapy rescues retinal degeneration in rd11 mice.
Residue Cys-211 of the conserved motif III is not essential for catalysis and not sufficient for sensitivity to treatment by sulfhydryl-modifier agents.
Title: Phosphatidylcholine formation by LPCAT1 is regulated by Ca(2+) and the redox status of the cell.
evidence of a protein substrate for Lpcat1 and reveal that histone lipidation may occur through its O-palmitoylation as a novel post-translational modification.
Title: Acyl-CoA:lysophosphatidylcholine acyltransferase I (Lpcat1) catalyzes histone protein O-palmitoylation to regulate mRNA synthesis.
LPS appears to destabilize the LPCAT1 protein by GSK-3beta-mediated phosphorylation within a canonical phosphodegron for beta-TrCP docking and site-specific ubiquitination.
Title: LPS impairs phospholipid synthesis by triggering beta-transducin repeat-containing protein (beta-TrCP)-mediated polyubiquitination and degradation of the surfactant enzyme acyl-CoA:lysophosphatidylcholine acyltransferase I (LPCAT1).
Studies add another dimension to an essential role for LPCAT1 in retinal photoreceptor homeostasis.
Title: Loss of lysophosphatidylcholine acyltransferase 1 leads to photoreceptor degeneration in rd11 mice.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Spontaneous (2) 1 citation
Gene trapped (1) 1 citation
Targeted (3) 1 citation
Endonuclease-mediated (5)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C87117 (e-PCR)
- UniSTS:208956

Lpcat1 (e-PCR), detects polymorphism
- UniSTS:531303

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 1-acylglycerol-3-phosphate O-acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables 1-acylglycerophosphocholine O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-acylglycerophosphocholine O-acyltransferase activity	ISO Inferred from Sequence Orthology more info
enables 1-alkenylglycerophosphocholine O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-alkylglycerophosphocholine O-acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-alkylglycerophosphocholine O-acyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables O-acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables lysophosphatidic acid acyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables plasmalogen synthase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of phosphatidylcholine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylcholine acyl-chain remodeling	ISO Inferred from Sequence Orthology more info
acts_upstream_of phosphatidylcholine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phospholipid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within retina development in camera-type eye	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within surfactant homeostasis	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in lipid droplet	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: lysophosphatidylcholine acyltransferase 1

Names: 1-acylglycerol-3-phosphate O-acyltransferase; 1-acylglycerophosphocholine O-acyltransferase; 1-alkenylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; LPC acyltransferase 1; acetyl-CoA:lyso-PAF acetyltransferase; acetyl-CoA:lyso-platelet-activating factor acetyltransferase; acyltransferase-like 2; lyso-PAF acetyltransferase; lysoPC acyltransferase 1

NP_001355610.1

EC 2.3.1.23

EC 2.3.1.25

EC 2.3.1.51

EC 2.3.1.67

NP_001355611.1

EC 2.3.1.23

EC 2.3.1.25

EC 2.3.1.51

EC 2.3.1.67

NP_663351.3

EC 2.3.1.23

EC 2.3.1.25

EC 2.3.1.51

EC 2.3.1.67

XP_006517252.1

EC 2.3.1.23

EC 2.3.1.25

EC 2.3.1.51

EC 2.3.1.67

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001368681.1 → NP_001355610.1 lysophosphatidylcholine acyltransferase 1 isoform 2

Status: VALIDATED

Source sequence(s)

AC154547

Conserved Domains (3) summary

cd07991
Location:1 → 212

LPLAT_LPCAT1-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LPCAT1-like

COG5126
Location:276 → 375

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]

pfam13833
Location:330 → 380

EF-hand_8; EF-hand domain pair
NM_001368682.1 → NP_001355611.1 lysophosphatidylcholine acyltransferase 1 isoform 3

Status: VALIDATED

Source sequence(s)

AC154547, AK046507

Conserved Domains (3) summary

cd07991
Location:1 → 111

LPLAT_LPCAT1-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LPCAT1-like

COG5126
Location:175 → 274

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]

pfam13833
Location:229 → 279

EF-hand_8; EF-hand domain pair
NM_145376.6 → NP_663351.3 lysophosphatidylcholine acyltransferase 1 isoform 1

See identical proteins and their annotated locations for NP_663351.3

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AC154547

Consensus CDS

CCDS36726.1

UniProtKB/Swiss-Prot

Q3TAX4, Q3TFD2, Q6NXZ6, Q8BG23, Q8BUX7, Q99JU6

Related

ENSMUSP00000022099.9, ENSMUST00000022099.15

Conserved Domains (5) summary

cd07991
Location:103 → 314

LPLAT_LPCAT1-like; Lysophospholipid Acyltransferases (LPLATs) of Glycerophospholipid Biosynthesis: LPCAT1-like

COG0204
Location:59 → 242

PlsC; 1-acyl-sn-glycerol-3-phosphate acyltransferase [Lipid transport and metabolism]

pfam13499
Location:421 → 479

EF-hand_7; EF-hand domain pair

pfam13833
Location:432 → 483

EF-hand_8; EF-hand domain pair

cl08302
Location:384 → 437

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

73615332..73664539

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006517189.4 → XP_006517252.1 lysophosphatidylcholine acyltransferase 1 isoform X1

Conserved Domains (3) summary

COG5126
Location:127 → 226

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]

pfam13833
Location:181 → 231

EF-hand_8; EF-hand domain pair

cl17185
Location:2 → 63

LPLAT; Lysophospholipid acyltransferases (LPLATs) of glycerophospholipid biosynthesis