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Spock1 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1 [ Mus musculus (house mouse) ]

Gene ID: 20745, updated on 21-Apr-2024

Summary

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Official Symbol
Spock1provided by MGI
Official Full Name
sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1provided by MGI
Primary source
MGI:MGI:105371
See related
Ensembl:ENSMUSG00000056222 AllianceGenome:MGI:105371
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Spock; Ticn1; testican
Summary
Predicted to enable calcium ion binding activity; cysteine-type endopeptidase inhibitor activity; and metalloendopeptidase inhibitor activity. Acts upstream of or within central nervous system neuron differentiation and neuron migration. Located in neuromuscular junction; node of Ranvier; and sarcoplasm. Is expressed in several structures, including jaw; nervous system; nose; skeletal musculature; and thymus primordium. Orthologous to human SPOCK1 (SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in cerebellum adult (RPKM 21.7), cortex adult (RPKM 19.5) and 7 other tissues See more
Orthologs
human all
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Genomic context

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Location:
13 B1; 13 30.5 cM
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 13 NC_000079.7 (57569008..58056194, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 13 NC_000079.6 (57421195..57908332, complement)

Chromosome 13 - NC_000079.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 1700072F12 gene Neighboring gene STARR-seq mESC enhancer starr_34662 Neighboring gene predicted gene, 48176 Neighboring gene predicted gene, 33294 Neighboring gene STARR-seq mESC enhancer starr_34663 Neighboring gene predicted gene, 33240 Neighboring gene STARR-seq mESC enhancer starr_34665 Neighboring gene STARR-seq mESC enhancer starr_34666 Neighboring gene STARR-seq mESC enhancer starr_34669 Neighboring gene CapStarr-seq enhancer MGSCv37_chr13:58168862-58169015 Neighboring gene kelch-like 3 Neighboring gene microRNA 874 Neighboring gene predicted gene, 33389

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Altered expression pattern of testican-1 mRNA after brain injury. Iseki K, et al. Biomed Res, 2011 Dec. PMID 22199127
  2. Testican-1 promotes resistance against Pseudomonas aeruginosa-induced keratitis through regulation of MMP-2 expression and activation. Berger EA, et al. Invest Ophthalmol Vis Sci, 2011 Jul 18. PMID 21613368, Free PMC Article
  3. Testican-1 is dispensable for mouse development. Röll S, et al. Matrix Biol, 2006 Aug. PMID 16806869
  4. Absence of SPARC in lens epithelial cells results in altered adhesion and extracellular matrix production in vitro. Weaver MS, et al. J Cell Biochem, 2006 Feb 1. PMID 16211577
  5. SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth. Alshargabi R, et al. Sci Rep, 2020 Jun 17. PMID 32555336, Free PMC Article

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The secreted neuronal signal Spock1 promotes blood-brain barrier development.
    Title: The secreted neuronal signal Spock1 promotes blood-brain barrier development.
  2. Testican-1 blood level is related to the severity of sepsis and Testican-1 could be used as a biomarker to determine the severity of sepsis.
    Title: Testican-1, as a novel diagnosis of sepsis.
  3. results suggest a possibility that testican-1 plays a permissive role for regenerating axons in reactive astrocytes after injury
    Title: Altered expression pattern of testican-1 mRNA after brain injury.
  4. Testican-1 is not critical for mouse development [review]
    Title: Testican-1 is dispensable for mouse development.
  5. limits the accumulation of adipose tissue in mice in part through its demonstrated effects on the regulation of cell shape and production of extracellular matrix
    Title: SPARC-null mice exhibit increased adiposity without significant differences in overall body weight.
  6. SPARC is counter-adhesive for murine lens epithelial cells. We also identify SPARC as a modulator of LN-1 secretion and deposition, an activity important in epithelial cell-ECM interactions in the ocular lens.
    Title: Absence of SPARC in lens epithelial cells results in altered adhesion and extracellular matrix production in vitro.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Targeted (1)  1 citation
  • Endonuclease-mediated (2) 

Pathways from PubChem

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General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables calcium ion binding ISO
Inferred from Sequence Orthology
more info
  
enables cysteine-type endopeptidase inhibitor activity ISO
Inferred from Sequence Orthology
more info
  
enables metalloendopeptidase inhibitor activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in central nervous system neuron differentiation IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in negative regulation of cell-substrate adhesion ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of endopeptidase activity ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of neuron projection development ISO
Inferred from Sequence Orthology
more info
  
involved_in neurogenesis IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in neuron migration IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extracellular region IEA
Inferred from Electronic Annotation
more info
  
located_in extracellular space ISO
Inferred from Sequence Orthology
more info
  
located_in neuromuscular junction IDA
Inferred from Direct Assay
more info
 PubMed 
located_in node of Ranvier IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in postsynaptic density IDA
Inferred from Direct Assay
more info
 PubMed 
located_in sarcoplasm IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
testican-1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001166463.1 → NP_001159935.1  testican-1 isoform 3 precursor

    See identical proteins and their annotated locations for NP_001159935.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks an in-frame small exon in the 5' coding region, as compared to variant 1. The resulting isoform (3) is 3 aa shorter than isoform 1.
    Source sequence(s)
    AC154748, AK051191, BY232939
    Consensus CDS
    CCDS49279.1
    UniProtKB/Swiss-Prot
    E9PYW3, Q62288
    UniProtKB/TrEMBL
    Q8BKQ3
    Related
    ENSMUSP00000140755.2, ENSMUST00000186271.7
    Conserved Domains (4) summary
    smart00280
    Location:137 → 178
    KAZAL; Kazal type serine protease inhibitors
    cd00191
    Location:312 → 373
    TY; Thyroglobulin type I repeats.; The N-terminal region of human thyroglobulin contains 11 type-1 repeats TY repeats are proposed to be inhibitors of cysteine proteases
    cd00252
    Location:197 → 315
    SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...
    pfam10591
    Location:196 → 302
    SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region

  2. NM_001166464.1 → NP_001159936.1  testican-1 isoform 4 precursor

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) has an alternate 3' sequence, as compared to variant 1. The resulting isoform (4) is shorter and has a distinct C-terminus, as compared to isoform 1.
    Source sequence(s)
    AK036189, AK039058
    Consensus CDS
    CCDS79194.1
    UniProtKB/TrEMBL
    A0A087WPP6, Q8BM19
    Related
    ENSMUSP00000139863.2, ENSMUST00000189373.8
    Conserved Domains (3) summary
    smart00280
    Location:140 → 181
    KAZAL; Kazal type serine protease inhibitors
    cd00252
    Location:200 → 318
    SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...
    pfam10591
    Location:199 → 305
    SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region

  3. NM_001166465.1 → NP_001159937.1  testican-1 isoform 5 precursor

    See identical proteins and their annotated locations for NP_001159937.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) lacks an in-frame small exon in the 5' coding region, and has an alternate 3' sequence, as compared to variant 1. The resulting isoform (5) is shorter and has a distinct C-terminus, as compared to isoform 1.
    Source sequence(s)
    AK036189, AK039058, BY232939
    Consensus CDS
    CCDS79193.1
    UniProtKB/TrEMBL
    Q8BM07, Q8BM19
    Related
    ENSMUSP00000141130.2, ENSMUST00000187852.7
    Conserved Domains (3) summary
    smart00280
    Location:137 → 178
    KAZAL; Kazal type serine protease inhibitors
    cd00252
    Location:197 → 315
    SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...
    pfam10591
    Location:196 → 302
    SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region

  4. NM_001166466.1 → NP_001159938.1  testican-1 isoform 2 precursor

    See identical proteins and their annotated locations for NP_001159938.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) has an alternate splice site, as compared to variant 1. The reading frame is not changed and the resulting isoform (2) lacks an internal aa, as compared to isoform 1.
    Source sequence(s)
    AC154748, BB628964, BC158020
    UniProtKB/Swiss-Prot
    E9PYW3, Q62288
    UniProtKB/TrEMBL
    B2RXY1
    Conserved Domains (4) summary
    smart00280
    Location:140 → 181
    KAZAL; Kazal type serine protease inhibitors
    cd00191
    Location:314 → 375
    TY; Thyroglobulin type I repeats.; The N-terminal region of human thyroglobulin contains 11 type-1 repeats TY repeats are proposed to be inhibitors of cysteine proteases
    cd00252
    Location:200 → 317
    SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...
    pfam10591
    Location:199 → 304
    SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region

  5. NM_009262.3 → NP_033288.2  testican-1 isoform 1 precursor

    See identical proteins and their annotated locations for NP_033288.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AC154748, BC075625, BY241184
    Consensus CDS
    CCDS79195.1
    UniProtKB/Swiss-Prot
    E9PYW3, Q62288
    UniProtKB/TrEMBL
    Q6DIC9
    Related
    ENSMUSP00000140409.2, ENSMUST00000185502.7
    Conserved Domains (4) summary
    smart00280
    Location:140 → 181
    KAZAL; Kazal type serine protease inhibitors
    cd00191
    Location:315 → 376
    TY; Thyroglobulin type I repeats.; The N-terminal region of human thyroglobulin contains 11 type-1 repeats TY repeats are proposed to be inhibitors of cysteine proteases
    cd00252
    Location:200 → 318
    SPARC_EC; SPARC_EC; extracellular Ca2+ binding domain (containing 2 EF-hand motifs) of SPARC and related proteins (QR1, SC1/hevin, testican and tsc-36/FRP). SPARC (BM-40) is a multifunctional glycoprotein, a matricellular protein, that functions to regulate ...
    pfam10591
    Location:199 → 305
    SPARC_Ca_bdg; Secreted protein acidic and rich in cysteine Ca binding region

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000079.7 Reference GRCm39 C57BL/6J

    Range
    57569008..58056194 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036157954.1 → XP_036013847.1  testican-1 isoform X1

    UniProtKB/Swiss-Prot
    E9PYW3, Q62288
    Conserved Domains (4) summary
    cd00191
    Location:311 → 372
    TY; Thyroglobulin type I repeats.; The N-terminal region of human thyroglobulin contains 11 type-1 repeats TY repeats are proposed to be inhibitors of cysteine proteases
    cd00252
    Location:244 → 275
    EFh_SPARC_EC; EF-hand motif [structural motif]
    cl25349
    Location:198 → 307
    EFh_SPARC_EC; EF-hand, extracellular calcium-binding (EC) motif, found in secreted protein acidic and rich in cysteine (SPARC)-like proteins
    pfam07648
    Location:136 → 173
    Kazal_2; Kazal-type serine protease inhibitor domain
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q62288.2 GenPept UniProtKB/Swiss-Prot:Q62288

Gene LinkOut

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