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Spam1 sperm adhesion molecule 1 [ Mus musculus (house mouse) ]

Gene ID: 20690, updated on 12-May-2024

Summary

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Official Symbol
Spam1provided by MGI
Official Full Name
sperm adhesion molecule 1provided by MGI
Primary source
MGI:MGI:109335
See related
Ensembl:ENSMUSG00000029682 AllianceGenome:MGI:109335
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Ph-20
Summary
Enables hyalurononglucosaminidase activity. Acts upstream of or within single fertilization. Located in acrosomal vesicle; external side of plasma membrane; and membrane raft. Orthologous to human SPAM1 (sperm adhesion molecule 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Restricted expression toward testis adult (RPKM 9.7) See more
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Genomic context

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Location:
6 A3.1; 6 11.28 cM
Exon count:
6
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (24791187..24801047)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (24791188..24801048)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene hyaluronoglucosaminidase 4 Neighboring gene predicted gene, 38776 Neighboring gene STARR-seq mESC enhancer starr_15360 Neighboring gene STARR-seq mESC enhancer starr_15361 Neighboring gene sperm adhesion molecule 1 pseudogene Neighboring gene hyaluronoglucosaminidase 5

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Murine SPAM1 is secreted by the estrous uterus and oviduct in a form that can bind to sperm during capacitation: acquisition enhances hyaluronic acid-binding ability and cumulus dispersal efficiency. Griffiths GS, et al. Reproduction, 2008 Mar. PMID 18299422
  2. Expression of SPAM1 (PH-20) in the murine kidney is not accompanied by hyaluronidase activity: evidence for potential roles in fluid and water reabsorption. Grigorieva A, et al. Kidney Blood Press Res, 2007. PMID 17446714
  3. Epididymal SPAM1 is a marker for sperm maturation in the mouse. Chen H, et al. Biol Reprod, 2006 May. PMID 16436526
  4. Murine Spam1 mRNA: involvement of AU-rich elements in the 3'UTR and antisense RNA in its tight post-transcriptional regulation in spermatids. Zhang H, et al. Mol Reprod Dev, 2006 Feb. PMID 16250006
  5. Spam1-associated transmission ratio distortion in mice: elucidating the mechanism. Martin-DeLeon PA, et al. Reprod Biol Endocrinol, 2005 Aug 10. PMID 16092963, Free PMC Article

See all (36) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A comparative analysis between WT and Spam1/Hyal5 dKO epididymal sperm revealed that the absence of these 2 sperm Hyals resulted in a marked accumulation of sperm on the outside of the cumulus-oocyte complex.
    Title: SPAM1/HYAL5 double deficiency in male mice leads to severe male subfertility caused by a cumulus-oocyte complex penetration defect.
  2. PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination.
    Title: Digestion products of the PH20 hyaluronidase inhibit remyelination.
  3. ACR or PRSS21 or both may function cooperatively with SPAM1 in sperm/cumulus penetration
    Title: Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization.
  4. The data from this paper suggests that the hyaluronan-degrading activity of the sperm hyaluronidase SPAM1 may not be required for fertilization, at least in the mouse.
    Title: Hyaluronan-degrading activity of mouse sperm hyaluronidase is not required for fertilization?
  5. SPAM1 may function in sperm entry into the cumulus and sperm penetration through the cumulus matrix.
    Title: Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization.
  6. First documentation of vesicular interaction with the sperm plasma membrane provides a model for the possible transfer mechanism of SPAM1 and other glycosyl phosphatidylinositol (GPI)-linked proteins to the sperm surface in reproductive fluids.
    Title: Investigating the role of murine epididymosomes and uterosomes in GPI-linked protein transfer to sperm using SPAM1 as a model.
  7. sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg
    Title: Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg.
  8. Sperm adhesion molecule 1 (SPAM1) is distributed in the nonciliated epithelial cells. Efferent ducts have the most intense staining. Spam1 was also present in the accessory organs, the prostate, and seminal vesicles and its fluid.
    Title: Spam1 (PH-20) expression in the extratesticular duct and accessory organs of the mouse: a possible role in sperm fluid reabsorption.
  9. Spam1 is a multifunctional protein and that it is less restricted in its expression than previously reported
    Title: Mouse Spam1 (PH-20) is a multifunctional protein: evidence for its expression in the female reproductive tract.
  10. SPAM1 is implicated in fluid reabsorption and urine concentration
    Title: Expression of SPAM1 (PH-20) in the murine kidney is not accompanied by hyaluronidase activity: evidence for potential roles in fluid and water reabsorption.
Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (1) 
  • Targeted (1)  1 citation

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables hyalurononglucosaminidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables hyalurononglucosaminidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables hyalurononglucosaminidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell adhesion IEA
Inferred from Electronic Annotation
more info
  
involved_in fusion of sperm to egg plasma membrane involved in single fertilization IEA
Inferred from Electronic Annotation
more info
  
involved_in hyaluronan catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within single fertilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in acrosomal vesicle IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in acrosomal vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in external side of plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane raft IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in side of membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
hyaluronidase PH-20
Names
hyal-PH20
hyaluronoglucosaminidase PH-20
sperm surface protein PH-20
NP_001073344.1
NP_033267.2
XP_006505088.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001079875.2 → NP_001073344.1  hyaluronidase PH-20 precursor

    See identical proteins and their annotated locations for NP_001073344.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AK005638, AY920282
    Consensus CDS
    CCDS19947.1
    UniProtKB/Swiss-Prot
    P48794, Q7TSD6, Q812F4, Q9DAQ1
    UniProtKB/TrEMBL
    Q5D1J0
    Related
    ENSMUSP00000143944.2, ENSMUST00000202331.4
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 369
    Glyco_hydro_56; Hyaluronidase

  2. NM_009241.3 → NP_033267.2  hyaluronidase PH-20 precursor

    See identical proteins and their annotated locations for NP_033267.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.
    Source sequence(s)
    AK005638, AY920282
    Consensus CDS
    CCDS19947.1
    UniProtKB/Swiss-Prot
    P48794, Q7TSD6, Q812F4, Q9DAQ1
    UniProtKB/TrEMBL
    Q5D1J0
    Related
    ENSMUSP00000143970.2, ENSMUST00000202569.4
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 369
    Glyco_hydro_56; Hyaluronidase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    24791187..24801047
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006505025.3 → XP_006505088.1  hyaluronidase PH-20 isoform X1

    See identical proteins and their annotated locations for XP_006505088.1

    UniProtKB/Swiss-Prot
    P48794, Q7TSD6, Q812F4, Q9DAQ1
    UniProtKB/TrEMBL
    Q5D1J0
    Related
    ENSMUSP00000031693.3, ENSMUST00000031693.3
    Conserved Domains (1) summary
    pfam01630
    Location:42 → 369
    Glyco_hydro_56; Hyaluronidase
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P48794.2 GenPept UniProtKB/Swiss-Prot:P48794

Gene LinkOut

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