Prkar2a protein kinase, cAMP dependent regulatory, type II alpha [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkar2aprovided by MGI
Official Full Name: protein kinase, cAMP dependent regulatory, type II alphaprovided by MGI
Primary source: MGI:MGI:108025
See related: Ensembl:ENSMUSG00000032601 AllianceGenome:MGI:108025
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: RII(alpha); 1110061A24Rik
Summary: Predicted to enable several functions, including beta-2 adrenergic receptor binding activity; cAMP binding activity; and enzyme binding activity. Involved in modulation of chemical synaptic transmission. Located in cytoplasm and glutamatergic synapse. Part of cAMP-dependent protein kinase complex. Is expressed in several structures, including 1st branchial arch; alimentary system; brain; eye; and genitourinary system. Human ortholog(s) of this gene implicated in congestive heart failure. Orthologous to human PRKAR2A (protein kinase cAMP-dependent type II regulatory subunit alpha). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in testis adult (RPKM 25.1), large intestine adult (RPKM 25.1) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F2; 9 59.63 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (108569342..108627643)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (108692143..108750444)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PRKAR2A deficiency protects mice from experimental colitis by increasing IFN-stimulated gene expression and modulating the intestinal microbiota.
Title: PRKAR2A deficiency protects mice from experimental colitis by increasing IFN-stimulated gene expression and modulating the intestinal microbiota.
Loss of habenular Prkar2a reduces hedonic eating and increases exercise motivation.
Title: Loss of habenular Prkar2a reduces hedonic eating and increases exercise motivation.
RII phosphorylation precedes cAMP binding and controls the inactivation by modulating the reassociation involving the coordinated action of phosphodiesterases and phosphatases.
Title: PKA-RII subunit phosphorylation precedes activation by cAMP and regulates activity termination.
PGD2-DP1 axis-induced M2 polarization facilitates resolution of inflammation through the PRKAR2A-mediated suppression of JAK2/STAT1 signaling.
Title: PKA regulatory IIα subunit is essential for PGD2-mediated resolution of inflammation.
Results show that mouse Prkar1a and human PRKAR2A exhibited a dynamic spatio-temporal expression in tooth development, whereas neither human PRKAR1A nor mouse Prkar2a showed their expression in odontogenesis.
Title: PKA regulatory subunit expression in tooth development.
Disruption of the ubiquitously expressed PKA RIIalpha subunit in mice confers resistance to diet-induced obesity, glucose intolerance, and hepatic steatosis.
Title: Differentially regulated protein kinase A (PKA) activity in adipose tissue and liver is associated with resistance to diet-induced obesity and glucose intolerance in mice that lack PKA regulatory subunit type IIα.
Results indicte that Cypher/ZASP interacted with the regulatory subunit RIIalpha of PKA.
Title: Cypher/ZASP is a novel A-kinase anchoring protein.
a key role for AKAP-targeted PKA in control of heart rate and contractile function
Title: Disruption of protein kinase A localization using a trans-activator of transcription (TAT)-conjugated A-kinase-anchoring peptide reduces cardiac function.
protein kinase cAMP dependent regulatory type II alpha showed a clear-cut double striation pattern on each m-line and z-line.
Title: PKA microdomain organisation and cAMP handling in healthy and dystrophic muscle in vivo.
AKAP121 and PKAR2A serve to enhance steroidogenesis by directing the synthesis and activation of STAR at the mitochondria in response to cAMP.
Title: Mitochondrial A-kinase anchoring protein 121 binds type II protein kinase A and enhances steroidogenic acute regulatory protein-mediated steroidogenesis in MA-10 mouse leydig tumor cells.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Prkar2a (e-PCR), detects polymorphism
- UniSTS:144260

RH136163 (e-PCR)
- UniSTS:210158

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables beta-2 adrenergic receptor binding	ISO Inferred from Sequence Orthology more info
enables cAMP binding	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP binding	ISO Inferred from Sequence Orthology more info
enables cAMP-dependent protein kinase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP-dependent protein kinase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables cAMP-dependent protein kinase regulator activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein kinase A catalytic subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase A catalytic subunit binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables small molecule binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cAMP-dependent protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cAMP-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein phosphorylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in T-tubule	ISO Inferred from Sequence Orthology more info
colocalizes_with axoneme	ISO Inferred from Sequence Orthology more info
part_of cAMP-dependent protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cAMP-dependent protein kinase complex	IDA Inferred from Direct Assay more info	PubMed
part_of cAMP-dependent protein kinase complex	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of nucleotide-activated protein kinase complex	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane raft	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
is_active_in synapse	EXP Inferred from Experiment more info	PubMed
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cAMP-dependent protein kinase type II-alpha regulatory subunit

NP_032950.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008924.3 → NP_032950.1 cAMP-dependent protein kinase type II-alpha regulatory subunit

See identical proteins and their annotated locations for NP_032950.1

Status: VALIDATED

Source sequence(s)

AC173341, CT571271

Consensus CDS

CCDS23536.1

UniProtKB/Swiss-Prot

P12367

UniProtKB/TrEMBL

Q8K1M3

Related

ENSMUSP00000035220.7, ENSMUST00000035220.12

Conserved Domains (2) summary

cd12103
Location:4 → 44

DD_RIIalpha_PKA; Dimerization/Docking domain of the Type II alpha Regulatory subunit of cAMP-dependent protein kinase

cd00038
Location:259 → 380

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...