Pou5f1 POU domain, class 5, transcription factor 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pou5f1provided by MGI
Official Full Name: POU domain, class 5, transcription factor 1provided by MGI
Primary source: MGI:MGI:101893
See related: Ensembl:ENSMUSG00000024406 AllianceGenome:MGI:101893
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Oct3; Oct4; Otf3; Otf4; NF-A3; Oct-3; Oct-4; Otf-3; Otf-4; Otf3g; Oct3/4; Oct-3/4; Otf3-rs7
Summary: The protein encoded by this gene belongs to the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein plays a key role in embryonic development and stem cell pluripotency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Expression: Biased expression in testis adult (RPKM 4.0) and ovary adult (RPKM 1.5) See more
Orthologs: human all
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Genomic context

Location:: 17 B1; 17 18.69 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (35816929..35821674)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (35506032..35510777)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Txnip regulates the Oct4-mediated pluripotency circuitry via metabolic changes upon differentiation.
Title: Txnip regulates the Oct4-mediated pluripotency circuitry via metabolic changes upon differentiation.
Regulatory Elements Outside Established Pou5f1 Gene Boundaries Are Required for Multilineage Differentiation of Embryonic Stem Cells.
Title: Regulatory Elements Outside Established Pou5f1 Gene Boundaries Are Required for Multilineage Differentiation of Embryonic Stem Cells.
Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance.
Title: Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance.
The homeodomain of Oct4 is a dimeric binder of methylated CpG elements.
Title: The homeodomain of Oct4 is a dimeric binder of methylated CpG elements.
Oct4 reduction contributes to testicular injury of unilateral testicular torsion in mice model and apoptotic death of Sertoli cells through mediating CIP2A expression.
Title: Oct4 reduction contributes to testicular injury of unilateral testicular torsion in mice model and apoptotic death of Sertoli cells through mediating CIP2A expression.
NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification.
Title: NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification.
OCT4 interprets and enhances nucleosome flexibility.
Title: OCT4 interprets and enhances nucleosome flexibility.
The Effect of Melatonin on OCT4 Expression and Granulosa Cell Growth in Female Mice.
Title: The Effect of Melatonin on OCT4 Expression and Granulosa Cell Growth in Female Mice.
Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency.
Title: Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency.
OCT4 activates a Suv39h1-repressive antisense lncRNA to couple histone H3 Lysine 9 methylation to pluripotency.
Title: OCT4 activates a Suv39h1-repressive antisense lncRNA to couple histone H3 Lysine 9 methylation to pluripotency.

Submit: New GeneRIF Correction See all GeneRIFs (328)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (11) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:516865

MGI:3033238 (e-PCR)
- UniSTS:274185

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:547852

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:226067

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:531513

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:474699

Oct4 (e-PCR)
- UniSTS:254301

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:498801

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:547698

Spp1 (e-PCR), detects polymorphism
- UniSTS:144503

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:144249

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:502650

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:525697

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:525717

UniSTS:464794 (e-PCR)
- UniSTS:464794

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:464794

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:502696

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:276943

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:548075

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:498923

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:546540

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:526834

Pou5f1 (e-PCR), detects polymorphism
- UniSTS:478966

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables HMG box domain binding	ISO Inferred from Sequence Orthology more info
enables POU domain binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IGI Inferred from Genetic Interaction more info	PubMed
enables RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables chromatin DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables cytokine binding	IDA Inferred from Direct Assay more info	PubMed
enables lysine-acetylated histone binding	IDA Inferred from Direct Assay more info	PubMed
enables miRNA binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info	PubMed
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in blastocyst development	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within blastocyst growth	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cell fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within ectodermal cell fate commitment	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within endodermal cell fate commitment	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within endodermal cell fate specification	ISO Inferred from Sequence Orthology more info	PubMed
involved_in gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within germ-line stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mesodermal cell fate commitment	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of calcium ion-dependent exocytosis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of asymmetric cell division	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to organic substance	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to retinoic acid	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within somatic stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within stem cell differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in stem cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stem cell population maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of trophectodermal cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within trophectodermal cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within trophectodermal cell fate commitment	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in female germ cell nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in germ cell nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in male germ cell nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IC Inferred by Curator more info	PubMed
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of transcription regulator complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	ISO Inferred from Sequence Orthology more info
part_of transcription repressor complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: POU domain, class 5, transcription factor 1

Names: octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; octamer-binding transcription factor 4 alternative

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001252452.1 → NP_001239381.1 POU domain, class 5, transcription factor 1 isoform 2

See identical proteins and their annotated locations for NP_001239381.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and uses a downstream, in-frame start codon, compared to variant 1. the encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AB375270, AW536651, BY390845

UniProtKB/TrEMBL

A0A2I6EDI9, A0A2I6EDJ6

Conserved Domains (2) summary

smart00352
Location:1 → 74

POU; Found in Pit-Oct-Unc transcription factors

pfam00046
Location:95 → 148

Homeobox; Homeobox domain
NM_013633.3 → NP_038661.2 POU domain, class 5, transcription factor 1 isoform 1

See identical proteins and their annotated locations for NP_038661.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK145321, BY390845, CU467494

Consensus CDS

CCDS37600.1

UniProtKB/Swiss-Prot

P20263, Q63843

Related

ENSMUSP00000025271.10, ENSMUST00000025271.17

Conserved Domains (2) summary

smart00352
Location:131 → 205

POU; Found in Pit-Oct-Unc transcription factors

pfam00046
Location:226 → 279

Homeobox; Homeobox domain