Plcb3 phospholipase C, beta 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Plcb3provided by MGI
Official Full Name: phospholipase C, beta 3provided by MGI
Primary source: MGI:MGI:104778
See related: Ensembl:ENSMUSG00000024960 AllianceGenome:MGI:104778
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: mKIAA4098
Summary: Predicted to enable calmodulin binding activity and phosphatidylinositol phospholipase C activity. Predicted to be involved in several processes, including inositol trisphosphate metabolic process; phosphatidylinositol catabolic process; and phosphatidylinositol-mediated signaling. Predicted to act upstream of or within regulation of systemic arterial blood pressure. Located in postsynaptic cytosol. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in spondylometaphyseal dysplasia with corneal dystrophy. Orthologous to human PLCB3 (phospholipase C beta 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in large intestine adult (RPKM 158.9), duodenum adult (RPKM 122.8) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 A; 19 5.1 cM

Exon count:: 33

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (6931081..6951738, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (6953708..6975006, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TRPM4 and PLCbeta3 contribute to normal behavioral responses to an array of sweeteners and carbohydrates but PLCbeta3 is not needed for taste-driven licking for glucose.
Title: TRPM4 and PLCβ3 contribute to normal behavioral responses to an array of sweeteners and carbohydrates but PLCβ3 is not needed for taste-driven licking for glucose.
A regulatory node involving Galphaq, PLCbeta, and RGS proteins modulates platelet reactivity to critical agonists.
Title: A regulatory node involving Gα(q), PLCβ, and RGS proteins modulates platelet reactivity to critical agonists.
Phospholipase C beta3 is Required for Climbing Fiber Synapse Elimination in Aldolase C-positive Compartments of the Developing Mouse Cerebellum.
Title: Phospholipase C β3 is Required for Climbing Fiber Synapse Elimination in Aldolase C-positive Compartments of the Developing Mouse Cerebellum.
Expression and localization of endogenous phospholipase Cbeta3 confined to basal cells in situ of immature ducts and adult excretory ducts of submandibular gland of mice.
Title: Expression and localization of endogenous phospholipase Cβ3 confined to basal cells in situ of immature ducts and adult excretory ducts of submandibular gland of mice.
Data, including data from studies using transgenic/knockout mice, suggest that Ppp1ca and Gnb1 interact in quiescent platelets; then, Ppp1ca and Plcb3 interact during platelet aggregation; thus, Gnb1 enlists Ppp1ca to modulate G protein-coupled receptor signaling. (Ppp1ca = protein phosphatase 1, catalytic subunit alpha; Gnb1 = guanine nucleotide-binding protein, subunit beta-1; Plcb3 = phospholipase C, subunit beta-3)
Title: The heterotrimeric G protein Gβ(1) interacts with the catalytic subunit of protein phosphatase 1 and modulates G protein-coupled receptor signaling in platelets.
Gnb isoforms control a signaling pathway comprising Rac1, Plcbeta2, and Plcbeta3 leading to LFA-1 activation and neutrophil arrest in vivo
Title: Gnb isoforms control a signaling pathway comprising Rac1, Plcβ2, and Plcβ3 leading to LFA-1 activation and neutrophil arrest in vivo.
Plcb3-deficient mice spontaneously develop atopic dermatitis-like skin lesions.
Title: Critical role for mast cell Stat5 activity in skin inflammation.
Data suggest that PLCbeta3 acts as a negative regulator of VEGF- (vascular endothelial growth factor A-) mediated vascular hyperpermeability through intracellular calcium signaling.
Title: Revealing the role of phospholipase Cβ3 in the regulation of VEGF-induced vascular permeability.
GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4.
Title: GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4.
defined a PLC-beta3- and SHP-1-mediated signaling pathway for FcvarepsilonRI-mediated cytokine production
Title: Phospholipase C-β3 regulates FcɛRI-mediated mast cell activation by recruiting the protein phosphatase SHP-1.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Plcb3 (e-PCR), detects polymorphism
- UniSTS:507151

Plcb3 (e-PCR), detects polymorphism
- UniSTS:144220

PLCB3sts2 (e-PCR)
- UniSTS:259010

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables molecular adaptor activity	ISO Inferred from Sequence Orthology more info
enables molecular function activator activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol phospholipase C activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol phospholipase C activity	ISO Inferred from Sequence Orthology more info
enables phosphoric diester hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in inositol trisphosphate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid catabolic process	IEA Inferred from Electronic Annotation more info
involved_in lipid catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol catabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylinositol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylinositol-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in post-Golgi vesicle-mediated transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of systemic arterial blood pressure	ISO Inferred from Sequence Orthology more info	PubMed
involved_in release of sequestered calcium ion into cytosol	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic cytosol	IEP Inferred from Expression Pattern more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info	PubMed
located_in sarcolemma	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Names: PLC-beta-3; phosphoinositide phospholipase C-beta-3

NP_001277278.1

EC 3.1.4.11

NP_032900.2

EC 3.1.4.11

XP_006526829.1

EC 3.1.4.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001290349.1 → NP_001277278.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform a

See identical proteins and their annotated locations for NP_001277278.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform a.

Source sequence(s)

AC120557, AI851116, AK146793

Consensus CDS

CCDS70925.1

UniProtKB/Swiss-Prot

P51432, Q3UIS0

UniProtKB/TrEMBL

Q3TWI4

Related

ENSMUSP00000025912.9, ENSMUST00000025912.10

Conserved Domains (6) summary

cd00275
Location:729 → 844

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08591
Location:316 → 694

PI-PLCc_beta; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-beta

cd13361
Location:22 → 149

PH_PLC_beta; Phospholipase C-beta (PLC-beta) pleckstrin homology (PH) domain

pfam08703
Location:1029 → 1202

PLC-beta_C; PLC-beta C terminal

pfam09279
Location:225 → 315

EF-hand_like; Phosphoinositide-specific phospholipase C, efhand-like

cl12013
Location:976 → 1118

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
NM_008874.4 → NP_032900.2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform b

See identical proteins and their annotated locations for NP_032900.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses two alternate splice sites that result in an alternate codon in the 5' coding region, compared to variant 1. The encoded isoform (b) is the same length as isoform a.

Source sequence(s)

AC120557, AI851116, AK146793, CB527492

UniProtKB/TrEMBL

Q3TWI4, Q8CI86

Conserved Domains (6) summary

cd00275
Location:729 → 844

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08591
Location:316 → 694

PI-PLCc_beta; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-beta

cd13361
Location:22 → 149

PH_PLC_beta; Phospholipase C-beta (PLC-beta) pleckstrin homology (PH) domain

pfam08703
Location:1029 → 1202

PLC-beta_C; PLC-beta C terminal

pfam09279
Location:225 → 315

EF-hand_like; Phosphoinositide-specific phospholipase C, efhand-like

cl12013
Location:976 → 1153

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

RNA

NR_177188.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC120557

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

6931081..6951738 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006526766.5 → XP_006526829.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X1

See identical proteins and their annotated locations for XP_006526829.1

UniProtKB/TrEMBL

Q3TWI4

Conserved Domains (6) summary

cd08591
Location:314 → 692

PI-PLCc_beta; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-beta

PLN02952
Location:316 → 832

PLN02952; phosphoinositide phospholipase C

cd13361
Location:32 → 147

PH_PLC_beta; Phospholipase C-beta (PLC-beta) pleckstrin homology (PH) domain

cd16210
Location:152 → 302

EFh_PI-PLCbeta3; EF-hand motif found in phosphoinositide phospholipase C beta 3 (PI-PLC-beta3)

pfam06631
Location:950 → 987

DUF1154; Protein of unknown function (DUF1154)

pfam08703
Location:1027 → 1200

PLC-beta_C; PLC-beta C terminal