Pfkfb2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pfkfb2provided by MGI
Official Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2provided by MGI
Primary source: MGI:MGI:107815
See related: Ensembl:ENSMUSG00000026409 AllianceGenome:MGI:107815
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 4930568D07Rik
Summary: Enables fructose-2,6-bisphosphate 2-phosphatase activity. Predicted to be involved in several processes, including fructose 2,6-bisphosphate metabolic process; monocarboxylic acid metabolic process; and positive regulation of glucokinase activity. Predicted to be located in cytoplasm and nucleoplasm. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and respiratory system. Orthologous to human PFKFB2 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in heart adult (RPKM 6.3), frontal lobe adult (RPKM 5.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 E4; 1 56.89 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (130616780..130656990, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (130689043..130729253, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss of Cardiac PFKFB2 Drives Metabolic, Functional, and Electrophysiological Remodeling in the Heart.
Title: Loss of Cardiac PFKFB2 Drives Metabolic, Functional, and Electrophysiological Remodeling in the Heart.
mTORC2-AKT signaling to PFKFB2 activates glycolysis that enhances stemness and tumorigenicity of intestinal epithelial cells.
Title: mTORC2-AKT signaling to PFKFB2 activates glycolysis that enhances stemness and tumorigenicity of intestinal epithelial cells.
PFKFB2 Inhibits Ferroptosis in Myocardial Ischemia/Reperfusion Injury Through Adenosine Monophosphate-Activated Protein Kinase Activation.
Title: PFKFB2 Inhibits Ferroptosis in Myocardial Ischemia/Reperfusion Injury Through Adenosine Monophosphate-Activated Protein Kinase Activation.
PFKFB2-mediated glycolysis promotes lactate-driven continual efferocytosis by macrophages.
Title: PFKFB2-mediated glycolysis promotes lactate-driven continual efferocytosis by macrophages.
Mutation of regulatory phosphorylation sites in PFKFB2 does not affect the anti-fibrotic effect of metformin in the kidney.
Title: Mutation of regulatory phosphorylation sites in PFKFB2 does not affect the anti-fibrotic effect of metformin in the kidney.
HIF-1/AKT Signaling-Activated PFKFB2 Alleviates Cardiac Dysfunction and Cardiomyocyte Apoptosis in Response to Hypoxia.
Title: HIF-1/AKT Signaling-Activated PFKFB2 Alleviates Cardiac Dysfunction and Cardiomyocyte Apoptosis in Response to Hypoxia.
PP2Acalpha inhibits PFKFB2-induced glycolysis to promote termination of liver regeneration.
Title: PP2Acα inhibits PFKFB2-induced glycolysis to promote termination of liver regeneration.
In diabetes mellitus type 1 loss of myocardial PFK-2 content as a result of reduced insulin signaling impairs the capacity to dynamically regulate glycolysis and elevates the levels of early glycolytic intermediates.
Title: Cardiac Insulin Signaling Regulates Glycolysis Through Phosphofructokinase 2 Content and Activity.
he dysfunction at disease onset was accompanied by increased expression of the rate-limiting glycolytic enzyme phosphofructokinase-2 in activated astrocytes, and by selective reduction in spinal mitochondrial complex I activity
Title: Mitochondrial dysfunction is an important cause of neurological deficits in an inflammatory model of multiple sclerosis.
SGK3 is not required for insulin-induced PFK-2 activation and that this effect is likely mediated by PKBalpha.
Title: Heart 6-phosphofructo-2-kinase activation by insulin requires PKB (protein kinase B), but not SGK3 (serum- and glucocorticoid-induced protein kinase 3).

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AU048745 (e-PCR)
- UniSTS:145195

Pfkfb2 (e-PCR), detects polymorphism
- UniSTS:546931

fl05f06.x1 (e-PCR)
- UniSTS:201902

X98847 (e-PCR)
- UniSTS:163260

fk56e12.x1 (e-PCR)
- UniSTS:201209

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 6-phosphofructo-2-kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 6-phosphofructo-2-kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 6-phosphofructo-2-kinase activity	ISO Inferred from Sequence Orthology more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables fructose-2,6-bisphosphate 2-phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables fructose-2,6-bisphosphate 2-phosphatase activity	ISO Inferred from Sequence Orthology more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within carbohydrate phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fructose 2,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glucose catabolic process	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process	ISO Inferred from Sequence Orthology more info
involved_in lactate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin secretion	ISO Inferred from Sequence Orthology more info
involved_in pyruvate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to glucose	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

Names: 6PF-2-K/Fru-2,6-P2ase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme; PFK-2/FBPase-2 gene B; PFK/FBPase 2

NP_001155887.1

EC 2.7.1.105

EC 3.1.3.46

NP_001155888.1

EC 2.7.1.105

EC 3.1.3.46

NP_001355772.1

EC 2.7.1.105

EC 3.1.3.46

NP_032851.2

EC 2.7.1.105

EC 3.1.3.46

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001162415.1 → NP_001155887.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform 2

See identical proteins and their annotated locations for NP_001155887.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, and differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC139231, AK029659, BC051014

Consensus CDS

CCDS48350.1

UniProtKB/TrEMBL

B2Z893, Q8CDS6

Related

ENSMUSP00000140355.2, ENSMUST00000189534.7

Conserved Domains (3) summary

pfam00300
Location:254 → 437

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:30 → 251

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:42 → 197

AAA_33; AAA domain
NM_001162416.1 → NP_001155888.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform 3

See identical proteins and their annotated locations for NP_001155888.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, and the 5' and 3' splice patterns have not been determined. The resulting isoform (3) lacks an internal segment near the C-terminus, compared to isoform 1.

Source sequence(s)

BC051014, EU616667

Consensus CDS

CCDS48349.1

UniProtKB/Swiss-Prot

P70265, Q8VEI9

UniProtKB/TrEMBL

B2Z892

Related

ENSMUSP00000133073.3, ENSMUST00000050406.11

Conserved Domains (3) summary

pfam00300
Location:254 → 406

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:30 → 251

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:42 → 197

AAA_33; AAA domain
NM_001368843.1 → NP_001355772.1 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform 4

Status: VALIDATED

Source sequence(s)

AC139231

Consensus CDS

CCDS87871.1

UniProtKB/TrEMBL

A0A087WRG0, A0A087WRM7

Related

ENSMUSP00000140612.2, ENSMUST00000187089.7

Conserved Domains (2) summary

pfam00300
Location:254 → 437

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:31 → 251

6PF2K; 6-phosphofructo-2-kinase
NM_008825.4 → NP_032851.2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 isoform 1

See identical proteins and their annotated locations for NP_032851.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and isoform 1.

Source sequence(s)

AC139231

Consensus CDS

CCDS35701.1

UniProtKB/Swiss-Prot

P70265, Q8VEI9

UniProtKB/TrEMBL

Q6GTL7

Related

ENSMUSP00000127587.3, ENSMUST00000169659.8

Conserved Domains (3) summary

pfam00300
Location:254 → 437

His_Phos_1; Histidine phosphatase superfamily (branch 1)

pfam01591
Location:30 → 251

6PF2K; 6-phosphofructo-2-kinase

pfam13671
Location:42 → 197

AAA_33; AAA domain

RNA

NR_027859.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses a distinct 3' splice pattern, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC139231, BC051014