Odf2 outer dense fiber of sperm tails 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Odf2provided by MGI
Official Full Name: outer dense fiber of sperm tails 2provided by MGI
Primary source: MGI:MGI:1098824
See related: Ensembl:ENSMUSG00000026790 AllianceGenome:MGI:1098824
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MMTEST29
Summary: Predicted to enable small GTPase binding activity. Predicted to be involved in several processes, including centriole-centriole cohesion; cilium organization; and regulation of cilium assembly. Predicted to act upstream of or within cell differentiation and spermatogenesis. Located in centriole; centrosome; and sperm flagellum. Part of ciliary transition fiber. Is expressed in several structures, including early conceptus; inner ear; liver; reproductive system; and stomach. Orthologous to human ODF2 (outer dense fiber of sperm tails 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in testis adult (RPKM 224.3), cerebellum adult (RPKM 22.9) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 B; 2 20.79 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (29779026..29821758)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (29889014..29931746)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Stable expression and multi-site location of Odf2 in mouse oocytes, sperm and early embryos.
Title: Stable expression and multi-site location of Odf2 in mouse oocytes, sperm and early embryos.
[Impacts of ODF2 gene knockdown on the sperm motility and fertility of male mice].
Title: [Impacts of ODF2 gene knockdown on the sperm motility and fertility of male mice].
BBOF1 is required for sperm motility and male fertility by stabilizing the flagellar axoneme in mice.
Title: BBOF1 is required for sperm motility and male fertility by stabilizing the flagellar axoneme in mice.
C3G localizes to the mother centriole in a cenexin-dependent manner and regulates centrosome duplication and primary cilium length.
Title: C3G localizes to the mother centriole in a cenexin-dependent manner and regulates centrosome duplication and primary cilium length.
Odf2 haploinsufficiency causes a new type of decapitated and decaudated spermatozoa, Odf2-DDS, in mice.
Title: Odf2 haploinsufficiency causes a new type of decapitated and decaudated spermatozoa, Odf2-DDS, in mice.
Odf2-deficiency causes sperm neck-midpiece separation, a new type of decapitated and decaudated spermatozoa (DDS), Odf2-DDS, whose semen contains abnormal spermatozoa with immotile tailless headnecks and motile headneckless tails.
Title: Odf2 haploinsufficiency causes a new type of decapitated and decaudated spermatozoa, Odf2-DDS, in mice.
Data show that the percentage of outer dense fiber of sperm tails 2 protein (ODF2) defects was higher in asthenozoospermic samples than that in control samples and was significantly correlated with the percentage of axoneme defects and non-motile sperm.
Title: Outer dense fibers stabilize the axoneme to maintain sperm motility.
The authors developed an Mrkn2 knockout mouse model to study the role of this gene, and found that deletion of Mkrn2 in mice led to male infertility and expression of Odf2, a vital protein in spermatogenesis, was significantly decreased.
Title: Deficiency of Mkrn2 causes abnormal spermiogenesis and spermiation, and impairs male fertility.
the C-terminal region (amino acids 214-638) of Odf2 is required for association with Tssk4; demonstrated the phosphorylation site in Odf2 by Tssk4
Title: Testis-specific serine/threonine protein kinase 4 (Tssk4) phosphorylates Odf2 at Ser-76.
Pax6 directly regulates the activity of the Odf2 gene encoding for the appendage-specific protein Odf2 with a role for the assembly of mother centriole.
Title: Pax6 controls centriole maturation in cortical progenitors through Odf2.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2) 1 citation
Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Odf2 (e-PCR), detects polymorphism
- UniSTS:515334

Odf2 (e-PCR), detects polymorphism
- UniSTS:515335

AI848335 (e-PCR)
- UniSTS:165157

D9Mit34.1 (e-PCR), detects polymorphism
- UniSTS:131903

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	ISO Inferred from Sequence Orthology more info
enables structural constituent of cytoskeleton	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in centriole-centriole cohesion	ISO Inferred from Sequence Orthology more info
involved_in cilium organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in protein localization	ISO Inferred from Sequence Orthology more info
involved_in regulation of cilium assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cilium assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within spermatogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
part_of centriolar subdistal appendage	ISO Inferred from Sequence Orthology more info
located_in centriole	IDA Inferred from Direct Assay more info	PubMed
located_in centriole	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in ciliary basal body	ISO Inferred from Sequence Orthology more info
part_of ciliary transition fiber	IDA Inferred from Direct Assay more info	PubMed
located_in cilium	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in motile cilium	IEA Inferred from Electronic Annotation more info
located_in outer dense fiber	ISO Inferred from Sequence Orthology more info
located_in sperm flagellum	IDA Inferred from Direct Assay more info	PubMed
located_in sperm midpiece	IDA Inferred from Direct Assay more info	PubMed
located_in sperm principal piece	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: outer dense fiber protein 2

Names: 84 kDa outer dense fiber protein; cenexin; outer dense fiber of sperm tails protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113213.2 → NP_001106684.1 outer dense fiber protein 2 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (1) uses a different splice site, compared to variant 4. The resulting protein (isoform a) is shorter by 1 aa when it is compared to isoform d. Variants 1 and 6 both encode the same isoform (a).

Source sequence(s)

AL928926

Consensus CDS

CCDS50556.1

UniProtKB/Swiss-Prot

A3KGV1

UniProtKB/TrEMBL

Q3UFG2

Related

ENSMUSP00000109385.2, ENSMUST00000113756.8

Conserved Domains (1) summary

COG1196
Location:114 → 802

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_001113214.2 → NP_001106685.1 outer dense fiber protein 2 isoform b

See identical proteins and their annotated locations for NP_001106685.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses a different splice site and contains a different 3' end, compared to variant 4. The resulting protein (isoform b) has a shorter and distinct C-terminus when it is compared to isoform d. Variants 2 and 7 both encode the same isoform (a).

Source sequence(s)

AL928926

Consensus CDS

CCDS50557.1

UniProtKB/TrEMBL

V9GXZ0

Related

ENSMUSP00000109384.2, ENSMUST00000113755.8

Conserved Domains (1) summary

COG1196
Location:114 → 446

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_001177659.2 → NP_001171130.1 outer dense fiber protein 2 isoform d

See identical proteins and their annotated locations for NP_001171130.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) encodes isoform d.

Source sequence(s)

AL928926

Consensus CDS

CCDS50555.1

UniProtKB/Swiss-Prot

A3KGV1

Related

ENSMUSP00000109388.3, ENSMUST00000113759.9

Conserved Domains (1) summary

COG1196
Location:114 → 803

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_001177661.2 → NP_001171132.1 outer dense fiber protein 2 isoform e

Status: VALIDATED

Description

Transcript Variant: This variant (5) has multiple differences that result in a different splice site and distinct 5' and 3' ends, compared to variant 4. The encoded protein (isoform c) has shorter and distinct termini when it is compared to isoform e.

Source sequence(s)

AL928926

Consensus CDS

CCDS50554.1

UniProtKB/TrEMBL

A3KGV9, A3KGW0

Related

ENSMUSP00000109396.2, ENSMUST00000113767.8

Conserved Domains (1) summary

cl25732
Location:163 → 495

SMC_N; RecF/RecN/SMC N terminal domain
NM_001355136.2 → NP_001342065.1 outer dense fiber protein 2 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (6), as well as variant 1, encodes isoform a.

Source sequence(s)

AL928926

Consensus CDS

CCDS50556.1

Related

ENSMUSP00000049272.8, ENSMUST00000046571.14

Conserved Domains (1) summary

COG1196
Location:114 → 802

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_001355137.2 → NP_001342066.1 outer dense fiber protein 2 isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (7), as well as variant 2, encodes isoform b.

Source sequence(s)

AL928926

Consensus CDS

CCDS50557.1

UniProtKB/TrEMBL

V9GXZ0

Conserved Domains (1) summary

COG1196
Location:114 → 446

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_001355138.2 → NP_001342067.1 outer dense fiber protein 2 isoform f

Status: VALIDATED

Source sequence(s)

AL928926

UniProtKB/TrEMBL

V9GXZ0

Conserved Domains (1) summary

cl25732
Location:95 → 427

SMC_N; RecF/RecN/SMC N terminal domain
NM_001369060.2 → NP_001355989.1 outer dense fiber protein 2 isoform g

Status: VALIDATED

Description

Transcript Variant: This variant (9), encodes the longest isoform (g).

Source sequence(s)

AL928926

Consensus CDS

CCDS89461.1

UniProtKB/Swiss-Prot

A3KGV1, A3KGV5, A3KGV6, O35135, O35496, Q3TH68, Q3UP80, Q6PGI6

Related

ENSMUSP00000109394.2, ENSMUST00000113765.8

Conserved Domains (1) summary

COG1196
Location:119 → 807

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_001374179.1 → NP_001361108.1 outer dense fiber protein 2 isoform h

Status: VALIDATED

Source sequence(s)

AL928926

Conserved Domains (1) summary

COG1196
Location:72 → 760

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_001374180.1 → NP_001361109.1 outer dense fiber protein 2 isoform a

Status: VALIDATED

Source sequence(s)

AL928926

Consensus CDS

CCDS50556.1

Related

ENSMUSP00000139390.2, ENSMUST00000184845.8

Conserved Domains (1) summary

COG1196
Location:114 → 802

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_001374181.1 → NP_001361110.1 outer dense fiber protein 2 isoform i

Status: VALIDATED

Source sequence(s)

AL928926

Consensus CDS

CCDS89462.1

Conserved Domains (1) summary

COG1196
Location:95 → 783

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_001374182.1 → NP_001361111.1 outer dense fiber protein 2 isoform i

Status: VALIDATED

Source sequence(s)

AL928926

Consensus CDS

CCDS89462.1

Related

ENSMUSP00000109386.2, ENSMUST00000113757.8

Conserved Domains (1) summary

COG1196
Location:95 → 783

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
NM_013615.4 → NP_038643.1 outer dense fiber protein 2 isoform c

See identical proteins and their annotated locations for NP_038643.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) has multiple differences that result in a different splice site and distinct 5' and 3' ends, compared to variant 4. The encoded protein (isoform c) has shorter and distinct termini when it is compared to isoform d.

Source sequence(s)

AL928926

Consensus CDS

CCDS15861.1

UniProtKB/TrEMBL

A3KGV9

Related

ENSMUSP00000028128.7, ENSMUST00000028128.13

Conserved Domains (1) summary

cl25732
Location:100 → 432

SMC_N; RecF/RecN/SMC N terminal domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

29779026..29821758

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017316279.2 → XP_017171768.1 outer dense fiber protein 2 isoform X4

Conserved Domains (1) summary

COG1196
Location:120 → 809

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036159407.1 → XP_036015300.1 outer dense fiber protein 2 isoform X5

Conserved Domains (1) summary

COG1196
Location:120 → 808

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_030248314.1 → XP_030104174.1 outer dense fiber protein 2 isoform X9

Conserved Domains (1) summary

COG1196
Location:101 → 789

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316291.1 → XP_017171780.1 outer dense fiber protein 2 isoform X7

UniProtKB/Swiss-Prot

A3KGV1

Conserved Domains (1) summary

COG1196
Location:114 → 803

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316301.1 → XP_017171790.1 outer dense fiber protein 2 isoform X12

Conserved Domains (1) summary

COG1196
Location:95 → 784

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036159440.1 → XP_036015333.1 outer dense fiber protein 2 isoform X13

Conserved Domains (1) summary

COG1196
Location:95 → 783

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036159428.1 → XP_036015321.1 outer dense fiber protein 2 isoform X8

Conserved Domains (1) summary

COG1196
Location:114 → 802

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316304.1 → XP_017171793.1 outer dense fiber protein 2 isoform X13

UniProtKB/Swiss-Prot

A3KGV1

Conserved Domains (1) summary

COG1196
Location:95 → 783

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316272.3 → XP_017171761.1 outer dense fiber protein 2 isoform X1

Conserved Domains (1) summary

COG1196
Location:163 → 852

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316275.3 → XP_017171764.1 outer dense fiber protein 2 isoform X2

Conserved Domains (1) summary

COG1196
Location:144 → 833

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316276.3 → XP_017171765.1 outer dense fiber protein 2 isoform X3

Conserved Domains (1) summary

COG1196
Location:144 → 832

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316300.2 → XP_017171789.1 outer dense fiber protein 2 isoform X12

Conserved Domains (1) summary

COG1196
Location:95 → 784

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036159427.1 → XP_036015320.1 outer dense fiber protein 2 isoform X8

Conserved Domains (1) summary

COG1196
Location:114 → 802

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316295.3 → XP_017171784.1 outer dense fiber protein 2 isoform X10

Conserved Domains (1) summary

COG1196
Location:100 → 789

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_030248309.2 → XP_030104169.1 outer dense fiber protein 2 isoform X6

UniProtKB/Swiss-Prot

A3KGV1, A3KGV5, A3KGV6, O35135, O35496, Q3TH68, Q3UP80, Q6PGI6

Conserved Domains (1) summary

COG1196
Location:119 → 807

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316292.2 → XP_017171781.1 outer dense fiber protein 2 isoform X7

UniProtKB/Swiss-Prot

A3KGV1

Conserved Domains (1) summary

COG1196
Location:114 → 803

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036159437.1 → XP_036015330.1 outer dense fiber protein 2 isoform X12

Conserved Domains (1) summary

COG1196
Location:95 → 784

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036159412.1 → XP_036015305.1 outer dense fiber protein 2 isoform X7

Conserved Domains (1) summary

COG1196
Location:114 → 803

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316290.3 → XP_017171779.1 outer dense fiber protein 2 isoform X7

UniProtKB/Swiss-Prot

A3KGV1

Conserved Domains (1) summary

COG1196
Location:114 → 803

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316294.3 → XP_017171783.1 outer dense fiber protein 2 isoform X10

Conserved Domains (1) summary

COG1196
Location:100 → 789

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316293.3 → XP_017171782.1 outer dense fiber protein 2 isoform X8

UniProtKB/Swiss-Prot

A3KGV1

Conserved Domains (1) summary

COG1196
Location:114 → 802

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_017316299.3 → XP_017171788.1 outer dense fiber protein 2 isoform X11

Conserved Domains (1) summary

COG1196
Location:100 → 788

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
XM_036159408.1 → XP_036015301.1 outer dense fiber protein 2 isoform X6

UniProtKB/Swiss-Prot

A3KGV1, A3KGV5, A3KGV6, O35135, O35496, Q3TH68, Q3UP80, Q6PGI6

Conserved Domains (1) summary

COG1196
Location:119 → 807

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]