U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Mgmt O-6-methylguanine-DNA methyltransferase [ Mus musculus (house mouse) ]

Gene ID: 17314, updated on 30-Apr-2024

Summary

Go to the top of the page Help
Official Symbol
Mgmtprovided by MGI
Official Full Name
O-6-methylguanine-DNA methyltransferaseprovided by MGI
Primary source
MGI:MGI:96977
See related
Ensembl:ENSMUSG00000054612 AllianceGenome:MGI:96977
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
AGT; Agat
Summary
Enables methyltransferase activity. Acts upstream of or within DNA dealkylation involved in DNA repair and regulation of cysteine-type endopeptidase activity involved in apoptotic process. Predicted to be located in nucleoplasm. Predicted to be active in nucleus. Is expressed in embryo and forelimb bud. Human ortholog(s) of this gene implicated in several diseases, including carcinoma (multiple); endocrine gland cancer (multiple); granulosa cell tumor; nervous system cancer (multiple); and urinary bladder cancer. Orthologous to human MGMT (O-6-methylguanine-DNA methyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in liver adult (RPKM 5.7), subcutaneous fat pad adult (RPKM 5.0) and 24 other tissues See more
Orthologs
human all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
7 F3; 7 82.07 cM
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (136496315..136732001)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (136894463..137128193)

Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene predicted gene 6249 Neighboring gene predicted gene, 29978 Neighboring gene STARR-seq mESC enhancer starr_20376 Neighboring gene 3-phosphoglycerate dehydrogenase pseudogene Neighboring gene STARR-seq mESC enhancer starr_20378 Neighboring gene predicted gene, 39108 Neighboring gene STARR-seq mESC enhancer starr_20380 Neighboring gene STARR-seq mESC enhancer starr_20381 Neighboring gene predicted gene, 30061 Neighboring gene early B cell factor 3 Neighboring gene predicted gene 10578

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Malignant progression in O6-methylguanine-DNA methyltransferase-deficient esophageal cancer cells is associated with Ezrin protein. Su Y, et al. DNA Cell Biol, 2012 May. PMID 22196440
  2. The cycad genotoxin MAM modulates brain cellular pathways involved in neurodegenerative disease and cancer in a DNA damage-linked manner. Kisby GE, et al. PLoS One, 2011. PMID 21731631, Free PMC Article
  3. Mgmt deficiency alters the in vivo mutational spectrum of tissues exposed to the tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Sandercock LE, et al. Carcinogenesis, 2008 Apr. PMID 18281247
  4. In vivo selection of hematopoietic stem cells transduced at a low multiplicity-of-infection with a foamy viral MGMT(P140K) vector. Cai S, et al. Exp Hematol, 2008 Mar. PMID 18279716, Free PMC Article
  5. Role of O6-methylguanine-DNA methyltransferase in protecting from alkylating agent-induced toxicity and mutations in mice. Hansen RJ, et al. Carcinogenesis, 2007 May. PMID 17116724

See all (74) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Circadian regulation of MGMT expression and promoter methylation underlies daily rhythms in TMZ sensitivity in glioblastoma.
    Title: Circadian regulation of MGMT expression and promoter methylation underlies daily rhythms in TMZ sensitivity in glioblastoma.
  2. Magnetic resonance imaging reveals specific anatomical changes in the brain of Agat- and Gamt-mice attributed to creatine depletion and guanidinoacetate alteration.
    Title: Magnetic resonance imaging reveals specific anatomical changes in the brain of Agat- and Gamt-mice attributed to creatine depletion and guanidinoacetate alteration.
  3. Muscle phenotype of AGAT- and GAMT-deficient mice after simvastatin exposure.
    Title: Muscle phenotype of AGAT- and GAMT-deficient mice after simvastatin exposure.
  4. This study shows that when cell death and cell cycle arrest pathways are inhibited, p53 can still mediate MGMT-dependent repair, to promote cell survival upon DNA damage.
    Title: The proline rich domain of p53 is dispensable for MGMT-dependent DNA repair and cell survival following alkylation damage.
  5. Snell, GHKRO, and PAPPA-KO mice express high levels of two proteins involved in DNA repair, O-6-methylguanine-DNA methyltransferase (MGMT) and N-myc downstream-regulated gene 1 (NDRG1).
    Title: mTOR regulates the expression of DNA damage response enzymes in long-lived Snell dwarf, GHRKO, and PAPPA-KO mice.
  6. This study demonstrates for the first time a non-linear dose-response for alkylation-induced colorectal carcinogenesis and reveals DNA repair by MGMT, but not AAG, as a key node in determining a carcinogenic threshold.
    Title: DNA repair by MGMT, but not AAG, causes a threshold in alkylation-induced colorectal carcinogenesis.
  7. Findings suggest that O6-methylguanine-DNA methyltransferase (MGMT)-mediated temozolomide resistance is associated with increased histone acetylation.
    Title: Inhibition of histone deacetylation potentiates the evolution of acquired temozolomide resistance linked to MGMT upregulation in glioblastoma xenografts.
  8. Loss of MGMT expression and high Ezrin protein expression leads to esophageal cancer.
    Title: Malignant progression in O6-methylguanine-DNA methyltransferase-deficient esophageal cancer cells is associated with Ezrin protein.
  9. Both base excision repair and O6-methylguanine-DNA methyltransferase protect against methylation-induced colon carcinogenesis.
    Title: Both base excision repair and O6-methylguanine-DNA methyltransferase protect against methylation-induced colon carcinogenesis.
  10. ta indicate that Zik1 and Gja9 demonstrated cancer-specific aberrant DNA methylation, whereas, Cdkn2a/p16, Igfbp3, Mgmt, Id4, and Cxcr4 were methylated in both the AOM tumors and normal colon mucosa.
    Title: Aberrant DNA methylation occurs in colon neoplasms arising in the azoxymethane colon cancer model.
Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Endonuclease-mediated (3) 
  • Targeted (5)  1 citation

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • MGC107020

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables calcium ion binding ISO
Inferred from Sequence Orthology
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylated-DNA-[protein]-cysteine S-methyltransferase activity ISO
Inferred from Sequence Orthology
more info
  
enables methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within DNA alkylation repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA alkylation repair ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within DNA damage response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA repair IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within DNA repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of apoptotic process ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of DNA repair ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of double-strand break repair ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within regulation of cysteine-type endopeptidase activity involved in apoptotic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

Go to the top of the page Help
Preferred Names
methylated-DNA--protein-cysteine methyltransferase
Names
6-O-methylguanine-DNA methyltransferase
O-6-alkylguanine-DNA alkyltransferase
O-6-methylguanine-DNA-alkyltransferase
NP_001363966.1
NP_001399626.1
NP_001399627.1
NP_001399628.1
NP_001399629.1
NP_001399630.1
NP_001399631.1
NP_001399632.1
NP_001399633.1
NP_032624.1
XP_006507472.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001377037.2 → NP_001363966.1  methylated-DNA--protein-cysteine methyltransferase isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC132594, AC135639, AC148997
    UniProtKB/Swiss-Prot
    P26187, Q54A49
    UniProtKB/TrEMBL
    Q4VA39
    Conserved Domains (1) summary
    COG0350
    Location:6 → 178
    AdaB; O6-methylguanine-DNA--protein-cysteine methyltransferase [Replication, recombination and repair]

  2. NM_001412697.1 → NP_001399626.1  methylated-DNA--protein-cysteine methyltransferase isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC132594, AC135639, AC148997

  3. NM_001412698.1 → NP_001399627.1  methylated-DNA--protein-cysteine methyltransferase isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997

  4. NM_001412699.1 → NP_001399628.1  methylated-DNA--protein-cysteine methyltransferase isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997

  5. NM_001412700.1 → NP_001399629.1  methylated-DNA--protein-cysteine methyltransferase isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997

  6. NM_001412701.1 → NP_001399630.1  methylated-DNA--protein-cysteine methyltransferase isoform 4

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997

  7. NM_001412702.1 → NP_001399631.1  methylated-DNA--protein-cysteine methyltransferase isoform 4

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997

  8. NM_001412703.1 → NP_001399632.1  methylated-DNA--protein-cysteine methyltransferase isoform 5

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997

  9. NM_001412704.1 → NP_001399633.1  methylated-DNA--protein-cysteine methyltransferase isoform 1

    Status: VALIDATED

    Source sequence(s)
    AC132594, AC135639, AC148997
    UniProtKB/Swiss-Prot
    P26187, Q54A49

  10. NM_008598.4 → NP_032624.1  methylated-DNA--protein-cysteine methyltransferase isoform 1

    See identical proteins and their annotated locations for NP_032624.1

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997
    Consensus CDS
    CCDS21945.1
    UniProtKB/Swiss-Prot
    P26187, Q54A49
    UniProtKB/TrEMBL
    Q4VA39
    Related
    ENSMUSP00000080224.3, ENSMUST00000081510.4
    Conserved Domains (1) summary
    COG0350
    Location:6 → 178
    AdaB; O6-methylguanine-DNA--protein-cysteine methyltransferase [Replication, recombination and repair]

RNA

  1. NR_182026.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997

  2. NR_182027.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997

  3. NR_182028.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC132122, AC132594, AC135639, AC148997

  4. NR_182029.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC132594, AC135639, AC148997

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000073.7 Reference GRCm39 C57BL/6J

    Range
    136496315..136732001
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006507409.4 → XP_006507472.1  methylated-DNA--protein-cysteine methyltransferase isoform X4

    See identical proteins and their annotated locations for XP_006507472.1

    UniProtKB/Swiss-Prot
    P26187, Q54A49
    UniProtKB/TrEMBL
    Q4VA39
    Conserved Domains (1) summary
    COG0350
    Location:6 → 178
    AdaB; O6-methylguanine-DNA--protein-cysteine methyltransferase [Replication, recombination and repair]
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P26187.3 GenPept UniProtKB/Swiss-Prot:P26187

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous