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Mcm3 minichromosome maintenance complex component 3 [ Mus musculus (house mouse) ]

Gene ID: 17215, updated on 5-Mar-2024

Summary

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Official Symbol
Mcm3provided by MGI
Official Full Name
minichromosome maintenance complex component 3provided by MGI
Primary source
MGI:MGI:101845
See related
Ensembl:ENSMUSG00000041859 AllianceGenome:MGI:101845
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
P1; Mcmd; p1.m; P1-MCM3
Summary
Predicted to enable single-stranded DNA binding activity. Predicted to contribute to single-stranded DNA helicase activity. Predicted to be involved in DNA strand elongation involved in DNA replication; double-strand break repair via break-induced replication; and mitotic DNA replication initiation. Located in cytoplasm and nucleus. Is expressed in several structures, including brain; limb; myocardium layer; neural ectoderm; and retina. Orthologous to human MCM3 (minichromosome maintenance complex component 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in liver E14 (RPKM 48.6), liver E14.5 (RPKM 44.8) and 18 other tissues See more
Orthologs
human all
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Genomic context

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Location:
1 A4; 1 6.5 cM
Exon count:
17
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (20873188..20890536, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (20802966..20820312, complement)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene interleukin 17A Neighboring gene STARR-seq mESC enhancer starr_00390 Neighboring gene interleukin 17F Neighboring gene STARR-positive B cell enhancer ABC_E1536 Neighboring gene chaperonin containing Tcp1, subunit 7 (eta) pseudogene Neighboring gene predicted gene, 24723

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. The murine replication protein P1 is differentially expressed during spermatogenesis. Starborg M, et al. Eur J Cell Biol, 1995 Oct. PMID 8575467
  2. DNA polymerase alpha associated protein P1, a murine homolog of yeast MCM3, changes its intranuclear distribution during the DNA synthetic period. Kimura H, et al. EMBO J, 1994 Sep 15. PMID 7925275, Free PMC Article
  3. Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex. Lin DI, et al. Proc Natl Acad Sci U S A, 2008 Jun 10. PMID 18524952, Free PMC Article
  4. Mouse pre-replicative complex proteins colocalise and interact with the centrosome. Stuermer A, et al. Eur J Cell Biol, 2007 Jan. PMID 17157410
  5. A murine replication protein accumulates temporarily in the heterochromatic regions of nuclei prior to initiation of DNA replication. Starborg M, et al. J Cell Sci, 1995 Mar. PMID 7622621

See all (71) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Study characterize the interaction of Keap1, a central antioxidant response regulator in Metazoa, with the replicative helicase subunit protein MCM3. Analysis suggests that structural determinants of the interaction of Keap1 with its critical downstream target - Nrf2 master transactivator of oxidative stress response genes - may have evolved in evolution to mimic the conserved helix-2-insert motif of MCM3.
    Title: Keap1-MCM3 interaction is a potential coordinator of molecular machineries of antioxidant response and genomic DNA replication in metazoa.
  2. Mcm3-deficient erythroblasts display aberrant DNA replication patterns and fail to complete maturation, causing lethal anemia.
    Title: Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality.
  3. These data reveal that CDK-dependent MCM3 phosphorylation contributes to the regulated formation of the MCM2-7 complex.
    Title: Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex.
  4. Depletion of Orc2 and Mcm3 by siRNA leads to an inhibition of cell proliferation, an altered cell cycle distribution as well as to multinucleated cells with insufficiently organised microtubules.
    Title: Mouse pre-replicative complex proteins colocalise and interact with the centrosome.
  5. interaction with GANP DNA-primase is associated with a novel phosphatase component G5PR
    Title: MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR.
  6. analysis of carboxyl-terminal MCM3 phosphorylation sites and their motifs
    Title: Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables single-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to single-stranded DNA helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in DNA duplex unwinding IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication initiation IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA strand elongation involved in DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA unwinding involved in DNA replication ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within cell cycle IEA
Inferred from Electronic Annotation
more info
  
involved_in double-strand break repair via break-induced replication IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitotic DNA replication initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in premeiotic DNA replication NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of CMG complex ISO
Inferred from Sequence Orthology
more info
  
part_of MCM complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of MCM complex ISO
Inferred from Sequence Orthology
more info
  
located_in centrosome ISO
Inferred from Sequence Orthology
more info
  
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus NAS
Non-traceable Author Statement
more info
 PubMed 
located_in perinuclear region of cytoplasm ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
DNA replication licensing factor MCM3
Names
DNA polymerase alpha holoenzyme-associated protein P1
mini chromosome maintenance deficient
minichromosome maintenance deficient 3
NP_032589.1
XP_030107786.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_008563.3 → NP_032589.1  DNA replication licensing factor MCM3

    See identical proteins and their annotated locations for NP_032589.1

    Status: VALIDATED

    Source sequence(s)
    AC156980, AC159614
    Consensus CDS
    CCDS14843.1
    UniProtKB/Swiss-Prot
    P25206, Q61492
    UniProtKB/TrEMBL
    Q3UI57, Q3ULD6, Q3UZH2
    Related
    ENSMUSP00000059192.10, ENSMUST00000053266.11
    Conserved Domains (3) summary
    smart00350
    Location:109 → 654
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:17 → 129
    MCM_N; MCM N-terminal domain
    cl21455
    Location:340 → 479
    P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    20873188..20890536 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030251926.2 → XP_030107786.1  DNA replication licensing factor MCM3 isoform X1

    UniProtKB/TrEMBL
    Q3UZH2
    Conserved Domains (1) summary
    smart00350
    Location:63 → 608
    MCM; minichromosome maintenance proteins
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25206.2 GenPept UniProtKB/Swiss-Prot:P25206

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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