Anpep alanyl aminopeptidase, membrane [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Anpepprovided by MGI
Official Full Name: alanyl aminopeptidase, membraneprovided by MGI
Primary source: MGI:MGI:5000466
See related: Ensembl:ENSMUSG00000039062 AllianceGenome:MGI:5000466
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Apn; AP-M; AP-N; Cd13; P150
Summary: Predicted to enable metalloaminopeptidase activity; peptide binding activity; and zinc ion binding activity. Predicted to be involved in several processes, including negative regulation of renal sodium excretion; peptide catabolic process; and proteolysis. Predicted to act upstream of or within angiogenesis and cell differentiation. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; brain; metanephros; reproductive system; and sensory organ. Orthologous to human ANPEP (alanyl aminopeptidase, membrane). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in large intestine adult (RPKM 1188.9), duodenum adult (RPKM 786.8) and 2 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 D2; 7 45.19 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (79471551..79497958, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (79821803..79848210, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CD13 facilitates immune cell migration and aggravates acute injury but promotes chronic post-stroke recovery.
Title: CD13 facilitates immune cell migration and aggravates acute injury but promotes chronic post-stroke recovery.
Soluble CD13 induces inflammatory arthritis by activating the bradykinin receptor B1.
Title: Soluble CD13 induces inflammatory arthritis by activating the bradykinin receptor B1.
CD13 is a critical regulator of cell-cell fusion in osteoclastogenesis.
Title: CD13 is a critical regulator of cell-cell fusion in osteoclastogenesis.
In Vivo Molecular Imaging of the Efficacy of Aminopeptidase N (APN/CD13) Receptor Inhibitor Treatment on Experimental Tumors Using (68)Ga-NODAGA-c(NGR) Peptide.
Title: In Vivo Molecular Imaging of the Efficacy of Aminopeptidase N (APN/CD13) Receptor Inhibitor Treatment on Experimental Tumors Using (68)Ga-NODAGA-c(NGR) Peptide.
ANPEP could be involved in the regulation of endometrial receptivity
Title: Aminopeptidase N expression in the endometrium could affect endometrial receptivity.
Findings indicate a role for cell surface antigen CD13 (CD13) in the fundamental cellular processes of receptor recycling cell-ECM interactions, and cell migration.
Title: CD13 tethers the IQGAP1-ARF6-EFA6 complex to the plasma membrane to promote ARF6 activation, β1 integrin recycling, and cell migration.
The histological data revealed the progression of liver cancer. Immunohistochemical analysis showed the strong expression of CD133 in metastatic cancer. In contrast, the expression of CD13 in both primary and metastatic liver cancer was found to be very strong.
Title: Aberrant expression of CD13 and stem cell markers in CD133-induced liver cancer in mice.
we concluded that APN plays a significant role in the regulation of several sperm functions and early embryonic development. In addition, increased APN activity could potentially lead to several adverse consequences related to male fertility.
Title: Elevated aminopeptidase N affects sperm motility and early embryo development.
These studies identified CD13 as a novel negative regulator of mast cell activation in vitro and in vivo.
Title: CD13/aminopeptidase N is a negative regulator of mast cell activation.
14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis.
Title: The pro-inflammatory cytokine 14-3-3ε is a ligand of CD13 in cartilage.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation
Endonuclease-mediated (8) 1 citation

General gene information

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Markers

U77083 (e-PCR)
- UniSTS:163421

Anpep (e-PCR), detects polymorphism
- UniSTS:498820

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables aminopeptidase activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloaminopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables peptide binding	IBA Inferred from Biological aspect of Ancestor more info
enables peptide binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within angiogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in peptide catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptide catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein processing	ISO Inferred from Sequence Orthology more info
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in brush border membrane	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum-Golgi intermediate compartment	ISO Inferred from Sequence Orthology more info
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: aminopeptidase N

Names: alanyl (membrane) aminopeptidase; alanyl aminopeptidase; aminopeptidase M; aminopeptidase N/CD13; membrane protein p161; microsomal aminopeptidase

NP_032512.2

EC 3.4.11.2

XP_036008613.1

EC 3.4.11.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008486.3 → NP_032512.2 aminopeptidase N

See identical proteins and their annotated locations for NP_032512.2

Status: VALIDATED

Source sequence(s)

AI848985, AK143746, AK171427, BY135516

Consensus CDS

CCDS21388.1

UniProtKB/Swiss-Prot

P97449, Q91YH8, Q99K63

UniProtKB/TrEMBL

Q3UP74

Related

ENSMUSP00000103015.2, ENSMUST00000107392.8

Conserved Domains (2) summary

cd09601
Location:84 → 545

M1_APN-Q_like; Peptidase M1 aminopeptidase N catalytic domain family which includes aminopeptidase N (APN), aminopeptidase Q (APQ), tricorn interacting factor F3, and endoplasmic reticulum aminopeptidase 1 (ERAP1)

pfam11838
Location:618 → 945

ERAP1_C; ERAP1-like C-terminal domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

79471551..79497958 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036152720.1 → XP_036008613.1 aminopeptidase N isoform X1

UniProtKB/Swiss-Prot

P97449, Q91YH8, Q99K63

UniProtKB/TrEMBL

Q3UP74

Related

ENSMUSP00000035943.7, ENSMUST00000049004.8

Conserved Domains (2) summary

cd09601
Location:84 → 545

M1_APN-Q_like; Peptidase M1 aminopeptidase N catalytic domain family which includes aminopeptidase N (APN), aminopeptidase Q (APQ), tricorn interacting factor F3, and endoplasmic reticulum aminopeptidase 1 (ERAP1)

pfam11838
Location:618 → 945

ERAP1_C; ERAP1-like C-terminal domain