Cst7 cystatin F (leukocystatin) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cst7provided by MGI
Official Full Name: cystatin F (leukocystatin)provided by MGI
Primary source: MGI:MGI:1298217
See related: Ensembl:ENSMUSG00000068129 AllianceGenome:MGI:1298217
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cmap
Summary: Enables cysteine-type endopeptidase inhibitor activity. Involved in negative regulation of peptidase activity. Acts upstream of or within inhibition of cysteine-type endopeptidase activity; negative regulation of microglial cell activation; and positive regulation of myelination. Located in several cellular components, including Golgi apparatus; late endosome; and lysosome. Is expressed in several structures, including early conceptus and female reproductive system. Orthologous to human CST7 (cystatin F). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in mammary gland adult (RPKM 5.2), spleen adult (RPKM 4.9) and 13 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 G3; 2 74.67 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (150412335..150420864)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (150570415..150578944)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cystatin F (Cst7) drives sex-dependent changes in microglia in an amyloid-driven model of Alzheimer's disease.
Title: Cystatin F (Cst7) drives sex-dependent changes in microglia in an amyloid-driven model of Alzheimer's disease.
Exploring the factors underlying remyelination arrest by studying the post-transcriptional regulatory mechanisms of cystatin F gene.
Title: Exploring the factors underlying remyelination arrest by studying the post-transcriptional regulatory mechanisms of cystatin F gene.
These results indicate that hypoxia BV2 cells highly express Cystatin F, which is involved in A2A R activation-mediated neuroinflammation via the PKA/CREB and PKC/CREB or ERK1/2 signaling pathways.
Title: Cystatin F involvement in adenosine A(2A) receptor-mediated neuroinflammation in BV2 microglial cells.
High CST7 expression is associated with Alzheimer's disease.
Title: RIPK1 mediates a disease-associated microglial response in Alzheimer's disease.
Our results validate cystatin F as a useful biomarker of early pathogenesis in experimental models of prion disease, and point to unexpected species-specific differences in the transcriptional responses to prion infections.
Title: Cystatin F is a biomarker of prion pathogenesis in mice.
During the active remyelinating phase, both CysF knockdown (CysFKD) and microglial-selective CatC overexpression (CatCOE) showed a worsening of the demyelination in Plp(4e/-) transgenic mice. Conversely, during the chronic demyelinating phase, CatC knockdown (CatCKD) ameliorated the demyelination. Our results suggest that the balance between CatC and CysF expression controls the demyelination and remyelination process.
Title: The balance between cathepsin C and cystatin F controls remyelination in the brain of Plp1-overexpressing mouse, a chronic demyelinating disease model.
The cystatin F expression in the activated microglia is closely associated with the effect of the A2A receptors, which may be related to the neuroinflammatory responses occurring during the pathological process.
Title: Adenosine A2A receptor deficiency up-regulates cystatin F expression in white matter lesions induced by chronic cerebral hypoperfusion.
an inhibitory activity other than cystatin F quenches CatL activity in activated macrophages
Title: Internalization of exogenous cystatin F supresses cysteine proteases and induces the accumulation of single-chain cathepsin L by multiple mechanisms.
Expression of cystatin F indicates ongoing demyelination and remyelination; absence of cystatin F expression indicates cessation of remyelination in the demyelinating area.
Title: Microglial cystatin F expression is a sensitive indicator for ongoing demyelination with concurrent remyelination.
The embryo's cystatin C and F expression functions as a protective mechanism against the maternal proteinase cathepsin S.
Title: The embryo's cystatin C and F expression functions as a protective mechanism against the maternal proteinase cathepsin S in mice.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (3)

General gene information

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Markers

NoName (e-PCR)
- UniSTS:491302

AB015224 (e-PCR)
- UniSTS:163301

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cysteine-type endopeptidase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables cysteine-type endopeptidase inhibitor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables peptidase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables peptidase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in immune response	IEA Inferred from Electronic Annotation more info
involved_in inhibition of cysteine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within inhibition of cysteine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of microglial cell activation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of microglial cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of peptidase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of peptidase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of myelination	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of myelination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IMP Inferred from Mutant Phenotype more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
is_active_in late endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in late endosome	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in lysosome	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosome	IMP Inferred from Mutant Phenotype more info	PubMed
located_in lysosome	ISO Inferred from Sequence Orthology more info
located_in multivesicular body	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cystatin-F

Names: cystatin-7; cystatin-like metastasis-associated protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.