U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Cd247 CD247 antigen [ Mus musculus (house mouse) ]

Gene ID: 12503, updated on 21-Apr-2024

Summary

Go to the top of the page Help
Official Symbol
Cd247provided by MGI
Official Full Name
CD247 antigenprovided by MGI
Primary source
MGI:MGI:88334
See related
Ensembl:ENSMUSG00000005763 AllianceGenome:MGI:88334
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Cd3; T3z; Cd3h; Cd3z; Tcrk; Tcrz; Cd3-eta; Cd3zeta; Cd3-zeta; 4930549J05Rik; A430104F18Rik
Summary
Predicted to enable identical protein binding activity and protein tyrosine kinase binding activity. Acts upstream of or within T cell receptor signaling pathway and positive regulation of protein localization to cell surface. Located in Golgi apparatus and plasma membrane. Part of alpha-beta T cell receptor complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and liver and biliary system. Human ortholog(s) of this gene implicated in immunodeficiency 25. Orthologous to human CD247 (CD247 molecule). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in thymus adult (RPKM 43.2), spleen adult (RPKM 5.1) and 2 other tissues See more
Orthologs
human all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
1 H2.3; 1 73.14 cM
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (165607514..165698722)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (165779952..165871153)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E11104 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:167648574-167648683 Neighboring gene STARR-positive B cell enhancer ABC_E1572 Neighboring gene predicted gene, 51752 Neighboring gene STARR-positive B cell enhancer ABC_E4374 Neighboring gene STARR-positive B cell enhancer ABC_E7771 Neighboring gene STARR-positive B cell enhancer ABC_E9064 Neighboring gene cellular repressor of E1A-stimulated genes 1 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:167732146-167732347 Neighboring gene predicted gene, 23402 Neighboring gene STARR-seq mESC enhancer starr_02912 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:167775596-167775783 Neighboring gene predicted gene, 32999 Neighboring gene POU domain, class 2, transcription factor 1 Neighboring gene STARR-seq mESC enhancer starr_02913 Neighboring gene STARR-positive B cell enhancer ABC_E3324 Neighboring gene STARR-positive B cell enhancer ABC_E9065 Neighboring gene STARR-positive B cell enhancer ABC_E2581 Neighboring gene STARR-positive B cell enhancer ABC_E4375 Neighboring gene STARR-positive B cell enhancer ABC_E4376 Neighboring gene STARR-positive B cell enhancer mm9_chr1:167878460-167878761 Neighboring gene heat shock protein 8 pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E2582 Neighboring gene STARR-seq mESC enhancer starr_02914 Neighboring gene STARR-positive B cell enhancer ABC_E798 Neighboring gene STARR-seq mESC enhancer starr_02915 Neighboring gene STARR-seq mESC enhancer starr_02916 Neighboring gene predicted gene, 53642

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Nanoclusters of the resting T cell antigen receptor (TCR) localize to non-raft domains. Beck-García K, et al. Biochim Biophys Acta, 2015 Apr. PMID 25535948
  2. Impaired spatial memory in mice lacking CD3ζ is associated with altered NMDA and AMPA receptors signaling independent of T-cell deficiency. Louveau A, et al. J Neurosci, 2013 Nov 20. PMID 24259588, Free PMC Article
  3. Unique ζ-chain motifs mediate a direct TCR-actin linkage critical for immunological synapse formation and T-cell activation. Klieger Y, et al. Eur J Immunol, 2014 Jan. PMID 24185712
  4. T cell CD3ζ deficiency enables multiorgan tissue inflammation. Deng GM, et al. J Immunol, 2013 Oct 1. PMID 23980209, Free PMC Article
  5. Basic residues in the T-cell receptor ζ cytoplasmic domain mediate membrane association and modulate signaling. Zhang H, et al. Proc Natl Acad Sci U S A, 2011 Nov 29. PMID 22084078, Free PMC Article

See all (172) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Alternatively Spliced Variants of Murine CD247 Influence T Cell Development and Activation, Revealing the Importance of the CD3zeta C-Terminal Region.
    Title: Alternatively Spliced Variants of Murine CD247 Influence T Cell Development and Activation, Revealing the Importance of the CD3ζ C-Terminal Region.
  2. CD3zeta ITAMs enable ligand discrimination and antagonism by inhibiting TCR signaling in response to low-affinity peptides.
    Title: CD3ζ ITAMs enable ligand discrimination and antagonism by inhibiting TCR signaling in response to low-affinity peptides.
  3. The CD3zeta adaptor structure determines functional differences between human and mouse CD16 Fc receptor signaling.
    Title: The CD3ζ adaptor structure determines functional differences between human and mouse CD16 Fc receptor signaling.
  4. Study findings underline the important roles of p38 and STAT3 in the regulation of PD-L1 expression and prove that IFNalpha induces STAT3/p38-mediated expression of PD-L1 and thereby a reduced stimulatory ability of dendritic cells.
    Title: Interferon-α Up-Regulates the Expression of PD-L1 Molecules on Immune Cells Through STAT3 and p38 Signaling.
  5. ITAM sequence diversity is required for optimal TCR signal transduction and subsequent T cell maturation
    Title: Cutting Edge: CD3 ITAM Diversity Is Required for Optimal TCR Signaling and Thymocyte Development.
  6. Comprehensive Survey of miRNA-mRNA Interactions Reveals That Ccr7 and Cd247 (CD3 zeta) are Posttranscriptionally Controlled in Pancreas Infiltrating T Lymphocytes of Non-Obese Diabetic (NOD) Mice
    Title: Comprehensive Survey of miRNA-mRNA Interactions Reveals That Ccr7 and Cd247 (CD3 zeta) are Posttranscriptionally Controlled in Pancreas Infiltrating T Lymphocytes of Non-Obese Diabetic (NOD) Mice.
  7. There is an early (within seconds) interaction between CD3zeta and the coreceptor CD8 that is independent of the binding of CD8 to MHC.
    Title: Ligand-engaged TCR is triggered by Lck not associated with CD8 coreceptor.
  8. The resting TCR localized in the disordered domain of giant plasma membrane (PM) vesicles (GPMVs) and PM spheres (PMSs) and single and nanoclustered TCRs are found in the high-density fractions in sucrose gradients.
    Title: Nanoclusters of the resting T cell antigen receptor (TCR) localize to non-raft domains.
  9. When Ly108 is engaged in trans during cell-cell interactions, Ly108-CD3zeta interactions are promoted in a manner that uniquely depends on Ly108 TM domain, leading to more efficient CD3zeta dephosphorylation.
    Title: SAP-regulated T Cell-APC adhesion and ligation-dependent and -independent Ly108-CD3ζ interactions.
  10. Two transcription factor binding sites and a long non-coding RNA are identified within the Cd247 gene.
    Title: Spatially conserved regulatory elements identified within human and mouse Cd247 gene using high-throughput sequencing data from the ENCODE project.
Submit: New GeneRIF Correction See all GeneRIFs (39)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (7)  1 citation
  • Chemically induced (ENU) (1) 
  • Endonuclease-mediated (1) 

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein heterodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables protein tyrosine kinase binding ISO
Inferred from Sequence Orthology
more info
  
enables transmembrane signaling receptor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in Fc-gamma receptor signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in T cell receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within T cell receptor signaling pathway IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within adaptive immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in cell surface receptor signaling pathway ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within immune system process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within interleukin-2 production IGI
Inferred from Genetic Interaction
more info
 PubMed 
acts_upstream_of_or_within positive regulation of protein localization to cell surface IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein complex oligomerization ISO
Inferred from Sequence Orthology
more info
  
involved_in protein-containing complex assembly ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of Fc-gamma receptor III complex ISO
Inferred from Sequence Orthology
more info
  
located_in Golgi apparatus IDA
Inferred from Direct Assay
more info
 PubMed 
part_of T cell receptor complex ISO
Inferred from Sequence Orthology
more info
  
part_of alpha-beta T cell receptor complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of alpha-beta T cell receptor complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of alpha-beta T cell receptor complex ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
CD247 antigen
Names
CD3 antigen, zeta polypeptide
CD3-zeta/eta
T-cell receptor T3 eta chain
T-cell surface glycoprotein CD3 zeta chain

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001113391.2 → NP_001106862.1  CD247 antigen isoform zeta precursor

    See identical proteins and their annotated locations for NP_001106862.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (zeta) encodes the shortest isoform (zeta).
    Source sequence(s)
    AC093371, AK138774, BC052824, BM240376
    Consensus CDS
    CCDS48427.1
    UniProtKB/Swiss-Prot
    P24161, P29020, Q9D3G3
    Related
    ENSMUSP00000005907.6, ENSMUST00000005907.12
    Conserved Domains (3) summary
    smart00077
    Location:69 → 89
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam02189
    Location:139 → 158
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam11628
    Location:28 → 58
    TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

  2. NM_001113392.2 → NP_001106863.1  CD247 antigen isoform iota precursor

    See identical proteins and their annotated locations for NP_001106863.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (iota) has alternate exon structure in the 3' coding region and 3' UTR, compared to variant zeta. The resulting isoform (iota) has a distinct C-terminus and is longer than isoform zeta.
    Source sequence(s)
    AC093371, AK138774
    Consensus CDS
    CCDS48429.1
    UniProtKB/TrEMBL
    Q3UU54
    Related
    ENSMUSP00000124297.2, ENSMUST00000161971.8
    Conserved Domains (2) summary
    smart00077
    Location:69 → 89
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam11628
    Location:28 → 58
    TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

  3. NM_001113393.2 → NP_001106864.2  CD247 antigen isoform theta precursor

    See identical proteins and their annotated locations for NP_001106864.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (theta) uses an alternate in-frame splice site in the central coding region, and has alternate exon structure in the 3' coding region and 3' UTR, compared to variant zeta. The resulting isoform (theta) has a distinct C-terminus and is longer than isoform zeta.
    Source sequence(s)
    AC093371, AC124587
    Consensus CDS
    CCDS48428.2
    UniProtKB/TrEMBL
    A2MZG6, E7EXC2, J3QMU3
    Related
    ENSMUSP00000124299.3, ENSMUST00000161559.3
    Conserved Domains (2) summary
    smart00077
    Location:69 → 89
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam11628
    Location:28 → 58
    TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

  4. NM_031162.4 → NP_112439.1  CD247 antigen isoform eta precursor

    See identical proteins and their annotated locations for NP_112439.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (eta) has alternate exon structure in the 3' coding region and 3' UTR, compared to variant zeta. The resulting isoform (eta) has a distinct C-terminus and is longer than isoform zeta. Variants eta and kappa encode the same isoform.
    Source sequence(s)
    AC093371, AC124587, BF020526
    Consensus CDS
    CCDS15443.1
    UniProtKB/TrEMBL
    J3QMU3
    Related
    ENSMUSP00000027849.5, ENSMUST00000027849.11
    Conserved Domains (2) summary
    smart00077
    Location:69 → 89
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam11628
    Location:28 → 58
    TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

RNA

  1. NR_103716.1 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (kappa) differs at the 3' end compared to variant zeta. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant zeta, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AC093371, AC124587

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    165607514..165698722
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011238745.4 → XP_011237047.1  CD247 antigen isoform X1

    See identical proteins and their annotated locations for XP_011237047.1

    UniProtKB/Swiss-Prot
    P24161, P29020, Q9D3G3
    Conserved Domains (3) summary
    smart00077
    Location:69 → 89
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam02189
    Location:139 → 158
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam11628
    Location:28 → 58
    TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

  2. XM_011238746.2 → XP_011237048.1  CD247 antigen isoform X2

    Conserved Domains (2) summary
    smart00077
    Location:69 → 89
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam11628
    Location:28 → 58
    TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

  3. XM_036156509.1 → XP_036012402.1  CD247 antigen isoform X1

    UniProtKB/Swiss-Prot
    P24161, P29020, Q9D3G3
    Conserved Domains (3) summary
    smart00077
    Location:69 → 89
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam02189
    Location:139 → 158
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam11628
    Location:28 → 58
    TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

  4. XM_017313285.2 → XP_017168774.1  CD247 antigen isoform X1

    UniProtKB/Swiss-Prot
    P24161, P29020, Q9D3G3
    Conserved Domains (3) summary
    smart00077
    Location:69 → 89
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam02189
    Location:139 → 158
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam11628
    Location:28 → 58
    TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

  5. XM_036156498.1 → XP_036012391.1  CD247 antigen isoform X1

    UniProtKB/Swiss-Prot
    P24161, P29020, Q9D3G3
    Conserved Domains (3) summary
    smart00077
    Location:69 → 89
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam02189
    Location:139 → 158
    ITAM; Immunoreceptor tyrosine-based activation motif
    pfam11628
    Location:28 → 58
    TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_001113394.2: Suppressed sequence

    Description
    NM_001113394.2: This RefSeq was permanently suppressed because the transcript is a nonsense-mediated mRNA decay (NMD) candidate when it uses a supported upstream start codon.
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P24161.1 GenPept UniProtKB/Swiss-Prot:P24161

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous