Brdt bromodomain, testis-specific [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Brdtprovided by MGI
Official Full Name: bromodomain, testis-specificprovided by MGI
Primary source: MGI:MGI:1891374
See related: Ensembl:ENSMUSG00000029279 AllianceGenome:MGI:1891374
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Brd6; Fsrg3; 7420412D09Rik
Summary: The protein encoded by this gene belongs to the BET protein family. BET proteins have two N-terminal bromodomains and one C-terminal extraterminal domain (ET domain). BET proteins regulate chromatin reorganization via binding to acetylated histones. This gene is thought to play a role in the transcriptional regulation of spermatogenesis. Although referred to as testis-specific bromodomain (Brdt) protein, RT-PCR indicates that this gene is expressed in both mouse oocytes and testes. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Jul 2008]
Expression: Biased expression in testis adult (RPKM 12.3), cortex adult (RPKM 1.6) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 E5; 5 52.23 cM

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (107478982..107534927)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (107331116..107387061)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Genome-wide chromatin occupancy of BRDT and gene expression analysis suggest transcriptional partners and specific epigenetic landscapes that regulate gene expression during spermatogenesis.
Title: Genome-wide chromatin occupancy of BRDT and gene expression analysis suggest transcriptional partners and specific epigenetic landscapes that regulate gene expression during spermatogenesis.
BRDT promotes ovarian cancer cell growth.
Title: BRDT promotes ovarian cancer cell growth.
BRDT controls the global chromatin organization and histone modifications of the chromatin attached to the synaptonemal complex. BRDT role in the silencing of sex chromosomes and crossover formation in male meiosis.
Title: BRDT is an essential epigenetic regulator for proper chromatin organization, silencing of sex chromosomes and crossover formation in male meiosis.
Our results suggest that in addition to its critical role in the spermatogenesis process, the BRDT protein is also responsible for scheduling male puberty by regulation of the pituitary-gonad axis.
Title: New insights into the role of the Brdt protein in the regulation of development and spermatogenesis in the mouse.
It was shown that Brdt is a unique and essential regulator of male germ cell differentiation, first by driving a specific spermatogenic gene expression program and then by controling the tight packaging of the male genome.
Title: Bromodomain-dependent stage-specific male genome programming by Brdt.
The N terminus of Brdt is involved in the recognition of Smarce1 as well as in the reorganization of hyperacetylated round spermatid chromatin.
Title: Insights into role of bromodomain, testis-specific (Brdt) in acetylated histone H4-dependent chromatin remodeling in mammalian spermiogenesis.
the first bromodomain of Brdt is critical in the formation and/or maintenance of an intact chromocenter and implicate this structure in proper remodeling of the chromatin architecture of the sperm head.
Title: The first bromodomain of the testis-specific double bromodomain protein Brdt is required for chromocenter organization that is modulated by genetic background.
a single bromodomain (BD1) of Brdt is responsible for selectively recognizing histone H4 tails bearing two or more acetylation marks
Title: Cooperative binding of two acetylation marks on a histone tail by a single bromodomain.
Homozygous Brdt(Delta)(BD1/)(Delta)(BD1) mice were viable but males were sterile, producing fewer and morphologically abnormal sperm.
Title: The first bromodomain of Brdt, a testis-specific member of the BET sub-family of double-bromodomain-containing proteins, is essential for male germ cell differentiation.
phylogenetic analyses show that the BRDT germ cell-specific orthology group clearly evolves faster than its ubiquitously expressed paralogs BRD2, BRD3, and BRD4
Title: Bromodomain testis-specific protein is expressed in mouse oocyte and evolves faster than its ubiquitously expressed paralogs BRD2, -3, and -4.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Brdt (e-PCR), detects polymorphism
- UniSTS:528254

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables histone binding	IDA Inferred from Direct Assay more info	PubMed
enables histone binding	ISO Inferred from Sequence Orthology more info
enables histone reader activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables lysine-acetylated histone binding	IBA Inferred from Biological aspect of Ancestor more info
enables lysine-acetylated histone binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within RNA splicing	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	ISO Inferred from Sequence Orthology more info
involved_in localization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in male meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within meiotic cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within_positive_effect positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of RNA splicing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sperm DNA condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of chromatin	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: bromodomain testis-specific protein

Names: RING3-like protein; bromodomain-containing female sterile homeotic-like protein; female sterile homeotic-related gene 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001079873.1 → NP_001073342.1 bromodomain testis-specific protein isoform B

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform B) that lacks the 3' bromodomain and ET domain, compared to isoform A.

Source sequence(s)

AK077137

Consensus CDS

CCDS39197.1

UniProtKB/Swiss-Prot

Q91Y44

Related

ENSMUSP00000108297.4, ENSMUST00000112677.10

Conserved Domains (2) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cl02556
Location:271 → 322

Bromodomain; Bromodomain. Bromodomains are found in many chromatin-associated proteins and in nuclear histone acetyltransferases. They interact specifically with acetylated lysine.
NM_054054.2 → NP_473395.2 bromodomain testis-specific protein isoform A

See identical proteins and their annotated locations for NP_473395.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (A).

Source sequence(s)

AF358660, AK039465, AK078663, BY724250, BY726053

Consensus CDS

CCDS19500.1

UniProtKB/Swiss-Prot

G3X8Z8, Q59HJ4, Q91Y44

Related

ENSMUSP00000031215.9, ENSMUST00000031215.15

Conserved Domains (4) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cd05498
Location:271 → 372

Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

pfam17035
Location:505 → 569

BET; Bromodomain extra-terminal - transcription regulation

pfam17105
Location:914 → 956

BRD4_CDT; C-terminal domain of bromodomain protein 4

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

107478982..107534927

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011249398.3 → XP_011247700.1 bromodomain testis-specific protein isoform X1

See identical proteins and their annotated locations for XP_011247700.1

Conserved Domains (4) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cd05498
Location:271 → 372

Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

pfam17035
Location:505 → 567

BET; Bromodomain extra-terminal - transcription regulation

pfam17105
Location:919 → 961

BRD4_CDT; C-terminal domain of bromodomain protein 4
XM_006534728.4 → XP_006534791.1 bromodomain testis-specific protein isoform X1

See identical proteins and their annotated locations for XP_006534791.1

Conserved Domains (4) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cd05498
Location:271 → 372

Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

pfam17035
Location:505 → 567

BET; Bromodomain extra-terminal - transcription regulation

pfam17105
Location:919 → 961

BRD4_CDT; C-terminal domain of bromodomain protein 4
XM_030254059.1 → XP_030109919.1 bromodomain testis-specific protein isoform X2

Conserved Domains (4) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cd05498
Location:271 → 372

Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

pfam17035
Location:505 → 567

BET; Bromodomain extra-terminal - transcription regulation

pfam17105
Location:918 → 960

BRD4_CDT; C-terminal domain of bromodomain protein 4
XM_030254060.1 → XP_030109920.1 bromodomain testis-specific protein isoform X3

Conserved Domains (4) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cd05498
Location:271 → 372

Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

pfam17035
Location:505 → 567

BET; Bromodomain extra-terminal - transcription regulation

pfam17105
Location:913 → 955

BRD4_CDT; C-terminal domain of bromodomain protein 4
XM_030254061.1 → XP_030109921.1 bromodomain testis-specific protein isoform X4

Conserved Domains (3) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cd05498
Location:271 → 372

Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

pfam17035
Location:505 → 567

BET; Bromodomain extra-terminal - transcription regulation
XM_006534730.4 → XP_006534793.1 bromodomain testis-specific protein isoform X1

See identical proteins and their annotated locations for XP_006534793.1

Conserved Domains (4) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cd05498
Location:271 → 372

Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

pfam17035
Location:505 → 567

BET; Bromodomain extra-terminal - transcription regulation

pfam17105
Location:919 → 961

BRD4_CDT; C-terminal domain of bromodomain protein 4
XM_030254058.1 → XP_030109918.1 bromodomain testis-specific protein isoform X1

Conserved Domains (4) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cd05498
Location:271 → 372

Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

pfam17035
Location:505 → 567

BET; Bromodomain extra-terminal - transcription regulation

pfam17105
Location:919 → 961

BRD4_CDT; C-terminal domain of bromodomain protein 4
XM_006534729.4 → XP_006534792.1 bromodomain testis-specific protein isoform X1

See identical proteins and their annotated locations for XP_006534792.1

Conserved Domains (4) summary

cd05497
Location:26 → 132

Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

cd05498
Location:271 → 372

Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...

pfam17035
Location:505 → 567

BET; Bromodomain extra-terminal - transcription regulation

pfam17105
Location:919 → 961

BRD4_CDT; C-terminal domain of bromodomain protein 4