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Cyb5r3 cytochrome b5 reductase 3 [ Mus musculus (house mouse) ]

Gene ID: 109754, updated on 21-Apr-2024

Summary

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Official Symbol
Cyb5r3provided by MGI
Official Full Name
cytochrome b5 reductase 3provided by MGI
Primary source
MGI:MGI:94893
See related
Ensembl:ENSMUSG00000018042 AllianceGenome:MGI:94893
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
B5R; Dia1; Dia-1; WU:Cyb5r3; 0610016L08Rik; 2500002N19Rik
Summary
Predicted to enable cytochrome-b5 reductase activity, acting on NAD(P)H and nucleotide binding activity. Predicted to contribute to nitrite reductase (NO-forming) activity. Predicted to be involved in nitric oxide biosynthetic process. Predicted to act upstream of or within cholesterol biosynthetic process. Located in mitochondrial inner membrane. Is expressed in several structures, including alimentary system; brain; cardiovascular system; genitourinary system; and hemolymphoid system gland. Human ortholog(s) of this gene implicated in breast cancer and methemoglobinemia. Orthologous to human CYB5R3 (cytochrome b5 reductase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in duodenum adult (RPKM 409.4), adrenal adult (RPKM 270.7) and 23 other tissues See more
Orthologs
human all
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Genomic context

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Location:
15 E1; 15 39.4 cM
Exon count:
12
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (83037696..83060641, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (83153495..83176440, complement)

Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene polymerase (DNA-directed), delta interacting protein 3 Neighboring gene RNA U12, small nuclear Neighboring gene STARR-positive B cell enhancer ABC_E4142 Neighboring gene STARR-seq mESC enhancer starr_39316 Neighboring gene STARR-seq mESC enhancer starr_39317 Neighboring gene alpha 1,4-galactosyltransferase Neighboring gene STARR-seq mESC enhancer starr_39321 Neighboring gene STARR-positive B cell enhancer ABC_E5503 Neighboring gene predicted gene, 32886

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling. Bueno M, et al. JCI Insight, 2023 Mar 8. PMID 36749633, Free PMC Article
  2. Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress. Durgin BG, et al. J Mol Cell Cardiol, 2022 Jan. PMID 34536439, Free PMC Article
  3. Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice. Rodríguez-López S, et al. Geroscience, 2020 Jun. PMID 32323139, Free PMC Article
  4. Loss of smooth muscle CYB5R3 amplifies angiotensin II-induced hypertension by increasing sGC heme oxidation. Durgin BG, et al. JCI Insight, 2019 Oct 3. PMID 31487266, Free PMC Article
  5. Crystal structure of a complex between amino and carboxy terminal fragments of mDia1: insights into autoinhibition of diaphanous-related formins. Nezami A, et al. PLoS One, 2010 Sep 30. PMID 20927338, Free PMC Article

See all (55) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Cyb5r3 activation rescues secondary failure to sulfonylurea but not beta-cell dedifferentiation.
    Title: Cyb5r3 activation rescues secondary failure to sulfonylurea but not β-cell dedifferentiation.
  2. CYB5R3 functions as a tumor suppressor by inducing ER stress-mediated apoptosis in lung cancer cells via the PERK-ATF4 and IRE1alpha-JNK pathways.
    Title: CYB5R3 functions as a tumor suppressor by inducing ER stress-mediated apoptosis in lung cancer cells via the PERK-ATF4 and IRE1α-JNK pathways.
  3. Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3.
    Title: Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b(5) reductase-3.
  4. Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.
    Title: Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes.
  5. The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.
    Title: The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.
  6. Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.
    Title: Smooth muscle cell CYB5R3 preserves cardiac and vascular function under chronic hypoxic stress.
  7. Cooperation between CYB5R3 and NOX4 via coenzyme Q mitigates endothelial inflammation.
    Title: Cooperation between CYB5R3 and NOX4 via coenzyme Q mitigates endothelial inflammation.
  8. Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.
    Title: Mitochondrial adaptations in liver and skeletal muscle to pro-longevity nutritional and genetic interventions: the crosstalk between calorie restriction and CYB5R3 overexpression in transgenic mice.
  9. GGPP depletion initiates metaflammation through disequilibrating CYB5R3-dependent eicosanoid metabolism.
    Title: GGPP depletion initiates metaflammation through disequilibrating CYB5R3-dependent eicosanoid metabolism.
  10. Cyb5r3 links FoxO1-dependent mitochondrial dysfunction with beta-cell failure.
    Title: Cyb5r3 links FoxO1-dependent mitochondrial dysfunction with β-cell failure.
Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (3)  1 citation
  • Endonuclease-mediated (2) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ADP binding ISO
Inferred from Sequence Orthology
more info
  
enables AMP binding ISO
Inferred from Sequence Orthology
more info
  
enables FAD binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables FAD binding ISO
Inferred from Sequence Orthology
more info
  
enables NAD binding ISO
Inferred from Sequence Orthology
more info
  
enables cytochrome-b5 reductase activity, acting on NAD(P)H ISO
Inferred from Sequence Orthology
more info
  
enables flavin adenine dinucleotide binding ISO
Inferred from Sequence Orthology
more info
  
contributes_to nitrite reductase (NO-forming) activity ISO
Inferred from Sequence Orthology
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within cholesterol biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cholesterol metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in nitric oxide biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within steroid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within steroid metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within sterol biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum membrane ISO
Inferred from Sequence Orthology
more info
  
located_in lipid droplet ISO
Inferred from Sequence Orthology
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrial inner membrane HDA PubMed 
located_in mitochondrial membrane ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial outer membrane ISO
Inferred from Sequence Orthology
more info
  
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of nitric-oxide synthase complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
NADH-cytochrome b5 reductase 3
Names
diaphorase 1 (NADH)
NP_084063.1
XP_006520345.2
XP_006520346.1
XP_030104133.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_029787.3 → NP_084063.1  NADH-cytochrome b5 reductase 3

    See identical proteins and their annotated locations for NP_084063.1

    Status: VALIDATED

    Source sequence(s)
    AI839369, BC043074
    Consensus CDS
    CCDS27698.1
    UniProtKB/Swiss-Prot
    Q9DCN2
    Related
    ENSMUSP00000018186.10, ENSMUST00000018186.16
    Conserved Domains (1) summary
    PLN02252
    Location:14 → 301
    PLN02252; nitrate reductase [NADPH]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000081.7 Reference GRCm39 C57BL/6J

    Range
    83037696..83060641 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_030248273.1 → XP_030104133.1  NADH-cytochrome b5 reductase 3 isoform X2

    UniProtKB/Swiss-Prot
    Q9DCN2
    Conserved Domains (1) summary
    PLN02252
    Location:14 → 301
    PLN02252; nitrate reductase [NADPH]

  2. XM_006520282.3 → XP_006520345.2  NADH-cytochrome b5 reductase 3 isoform X1

    Conserved Domains (1) summary
    PLN02252
    Location:33 → 320
    PLN02252; nitrate reductase [NADPH]

  3. XM_006520283.5 → XP_006520346.1  NADH-cytochrome b5 reductase 3 isoform X3

    UniProtKB/Swiss-Prot
    Q9DCN2
    Related
    ENSMUSP00000124062.3, ENSMUST00000162834.3
    Conserved Domains (1) summary
    PLN02252
    Location:15 → 278
    PLN02252; nitrate reductase [NADPH]
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9DCN2.3 GenPept UniProtKB/Swiss-Prot:Q9DCN2

Gene LinkOut

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