Prkab2 protein kinase, AMP-activated, beta 2 non-catalytic subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkab2provided by MGI
Official Full Name: protein kinase, AMP-activated, beta 2 non-catalytic subunitprovided by MGI
Primary source: MGI:MGI:1336185
See related: Ensembl:ENSMUSG00000038205 AllianceGenome:MGI:1336185
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 5730553K21Rik
Summary: Predicted to enable protein kinase binding activity. Predicted to contribute to AMP-activated protein kinase activity. Involved in positive regulation of cold-induced thermogenesis. Predicted to be located in apical plasma membrane. Predicted to be part of cAMP-dependent protein kinase complex and nucleotide-activated protein kinase complex. Predicted to be active in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; cardiovascular system; gallbladder; genitourinary system; and nervous system. Orthologous to human PRKAB2 (protein kinase AMP-activated non-catalytic subunit beta 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in bladder adult (RPKM 27.2), heart adult (RPKM 10.1) and 23 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 F2.2; 3 42.25 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (97565527..97581128)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (97658211..97673812)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

these data indicate that increased expression of AMPKbeta2 is an important feature of efficient adipogenesis.
Title: AMP-activated protein kinase complexes containing the β2 regulatory subunit are up-regulated during and contribute to adipogenesis.
the evidence indicates that AMPK is sufficient but not essential for the maintenance of DAPC expression in skeletal muscle, yet it is required for preserving extrasynaptic utrophin levels in slow oxidative muscles.
Title: The role of AMP-activated protein kinase in the expression of the dystrophin-associated protein complex in skeletal muscle.
AMPK directly relaxes vascular smooth muscle cell by a decrease of [Ca(2+)]i. This is achieved by calcium sequestration via SERCA activation, as well as activation of BKCa channels.
Title: AMPK Dilates Resistance Arteries via Activation of SERCA and BKCa Channels in Smooth Muscle.
AMPK beta1beta2 have a role in preventing myopathy due to loss of capillary density in nonpostural muscles
Title: Muscle-specific AMPK β1β2-null mice display a myopathy due to loss of capillary density in nonpostural muscles.
The beta2 subunit plays an important role in regulating glucose, glycogen, and lipid metabolism during metabolic stress. beta2 mutant animals failed to maintain euglycemia and muscle ATP levels during fasting.
Title: The AMPK β2 subunit is required for energy homeostasis during metabolic stress.
Data show that beta1beta2M-KO mice are physically inactive and have a drastically impaired capacity for treadmill running that is associated with reductions in skeletal muscle mitochondrial content.
Title: AMP-activated protein kinase (AMPK) beta1beta2 muscle null mice reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise.
Deletion of AMPK beta2 reduces AMPK activity in skeletal muscle resulting in impaired exercise capacity.
Title: Whole body deletion of AMP-activated protein kinase {beta}2 reduces muscle AMPK activity and exercise capacity.
Germline deletion of either AMPK beta1 or beta2 subunit isoforms resulted in reduced trabecular bone density and mass, but without effects on osteoclast (OC) or osteoblast (OB) numbers.
Title: Germline deletion of AMP-activated protein kinase beta subunits reduces bone mass without altering osteoclast differentiation or function.
Results suggest that p38 MAPK is not a downstream component of AMPK-mediated signaling in skeletal muscle.
Title: Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Gene trapped (1)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:234769

NoName (e-PCR)
- UniSTS:234770

BB124140 (e-PCR)
- UniSTS:185554

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
contributes_to AMP-activated protein kinase activity	ISO Inferred from Sequence Orthology more info
enables AMP-activated protein kinase activity	TAS Traceable Author Statement more info	PubMed
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to nutrient levels	ISO Inferred from Sequence Orthology more info
involved_in cellular response to nutrient levels	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within fatty acid biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within fatty acid metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cold-induced thermogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
part_of cAMP-dependent protein kinase complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleoplasm	TAS Traceable Author Statement more info
part_of nucleotide-activated protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nucleotide-activated protein kinase complex	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: 5'-AMP-activated protein kinase subunit beta-2

Names: AMPK subunit beta-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_182997.2 → NP_892042.2 5'-AMP-activated protein kinase subunit beta-2

See identical proteins and their annotated locations for NP_892042.2

Status: PROVISIONAL

Source sequence(s)

BC060228

Consensus CDS

CCDS17657.1

UniProtKB/Swiss-Prot

Q6PAM0

Related

ENSMUSP00000036410.7, ENSMUST00000045743.13

Conserved Domains (2) summary

smart01010
Location:182 → 271

AMPKBI; 5'-AMP-activated protein kinase beta subunit, interation domain

pfam16561
Location:76 → 158

AMPK1_CBM; Glycogen recognition site of AMP-activated protein kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

97565527..97581128

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030252348.1 → XP_030108208.1 5'-AMP-activated protein kinase subunit beta-2 isoform X2

Conserved Domains (2) summary

smart01010
Location:138 → 227

AMPKBI; 5'-AMP-activated protein kinase beta subunit, interation domain

pfam16561
Location:32 → 114

AMPK1_CBM; Glycogen recognition site of AMP-activated protein kinase
XM_011239982.4 → XP_011238284.1 5'-AMP-activated protein kinase subunit beta-2 isoform X1

See identical proteins and their annotated locations for XP_011238284.1

UniProtKB/Swiss-Prot

Q6PAM0

Conserved Domains (2) summary

smart01010
Location:182 → 271

AMPKBI; 5'-AMP-activated protein kinase beta subunit, interation domain

pfam16561
Location:76 → 158

AMPK1_CBM; Glycogen recognition site of AMP-activated protein kinase
XM_006500840.3 → XP_006500903.1 5'-AMP-activated protein kinase subunit beta-2 isoform X1

See identical proteins and their annotated locations for XP_006500903.1

UniProtKB/Swiss-Prot

Q6PAM0

Conserved Domains (2) summary

smart01010
Location:182 → 271

AMPKBI; 5'-AMP-activated protein kinase beta subunit, interation domain

pfam16561
Location:76 → 158

AMPK1_CBM; Glycogen recognition site of AMP-activated protein kinase
XM_006500841.3 → XP_006500904.1 5'-AMP-activated protein kinase subunit beta-2 isoform X2

Conserved Domains (2) summary

smart01010
Location:138 → 227

AMPKBI; 5'-AMP-activated protein kinase beta subunit, interation domain

pfam16561
Location:32 → 114

AMPK1_CBM; Glycogen recognition site of AMP-activated protein kinase