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Mgat5 mannoside acetylglucosaminyltransferase 5 [ Mus musculus (house mouse) ]

Gene ID: 107895, updated on 5-Mar-2024

Summary

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Official Symbol
Mgat5provided by MGI
Official Full Name
mannoside acetylglucosaminyltransferase 5provided by MGI
Primary source
MGI:MGI:894701
See related
Ensembl:ENSMUSG00000036155 AllianceGenome:MGI:894701
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
GlcNAc-TV; 4930471A21Rik; 5330407H02Rik
Summary
Enables alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity. Acts upstream of or within protein N-linked glycosylation. Located in Golgi apparatus. Is expressed in several structures, including alimentary system; brain; integumental system; sensory organ; and urinary system. Orthologous to human MGAT5 (alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in colon adult (RPKM 9.9), frontal lobe adult (RPKM 9.4) and 27 other tissues See more
Orthologs
human all
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Genomic context

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See Mgat5 in Genome Data Viewer
Location:
1 E3; 1 55.71 cM
Exon count:
20
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (127132450..127413760)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (127204919..127488334)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 41945 Neighboring gene ribosomal protein S2 pseudogene Neighboring gene STARR-seq mESC enhancer starr_02119 Neighboring gene STARR-seq mESC enhancer starr_02121 Neighboring gene STARR-seq mESC enhancer starr_02130 Neighboring gene predicted gene 8451 Neighboring gene predicted gene, 51747 Neighboring gene STARR-positive B cell enhancer mm9_chr1:129277748-129278049 Neighboring gene predicted gene, 23370 Neighboring gene STARR-seq mESC enhancer starr_02135 Neighboring gene STARR-positive B cell enhancer mm9_chr1:129321878-129322179 Neighboring gene predicted gene, 23734 Neighboring gene predicted gene, 51746 Neighboring gene STARR-positive B cell enhancer mm9_chr1:129355594-129355895 Neighboring gene STARR-positive B cell enhancer ABC_E3299 Neighboring gene predicted gene, 41947 Neighboring gene STARR-seq mESC enhancer starr_02141 Neighboring gene STARR-positive B cell enhancer ABC_E3300 Neighboring gene transmembrane protein 163 Neighboring gene VISTA enhancer mm790 Neighboring gene STARR-positive B cell enhancer ABC_E9476 Neighboring gene STARR-positive B cell enhancer mm9_chr1:129576446-129576747 Neighboring gene STARR-seq mESC enhancer starr_02145 Neighboring gene predicted gene, 35993 Neighboring gene amino carboxymuconate semialdehyde decarboxylase

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation. Yale AR, et al. Stem Cell Reports, 2023 Jun 13. PMID 37172586, Free PMC Article
  2. Increasing cell permeability of N-acetylglucosamine via 6-acetylation enhances capacity to suppress T-helper 1 (TH1)/TH17 responses and autoimmunity. Lee SU, et al. PLoS One, 2019. PMID 30913278, Free PMC Article
  3. Metabolic control of T cell immune response through glycans in inflammatory bowel disease. Dias AM, et al. Proc Natl Acad Sci U S A, 2018 May 15. PMID 29720442, Free PMC Article
  4. Mgat5 modulates the effect of early life stress on adult behavior and physical health in mice. Feldcamp L, et al. Behav Brain Res, 2016 Oct 1. PMID 27329152
  5. N-Acetylglucosaminyltransferase V exacerbates murine colitis with macrophage dysfunction and enhances colitic tumorigenesis. Shinzaki S, et al. J Gastroenterol, 2016 Apr. PMID 26349931

See all (68) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Loss of N-acetylglucosaminyl transferase V is involved in the impaired osteogenic differentiation of bone marrow mesenchymal stem cells.
    Title: Loss of N-acetylglucosaminyl transferase V is involved in the impaired osteogenic differentiation of bone marrow mesenchymal stem cells.
  2. Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.
    Title: Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation.
  3. Deficiency in Branched Glycosylation in MGAT5 Null or Heterozygous Mice Is Associated with Early-Onset Disease and Increased Severity of Colitis>
    Title: Metabolic control of T cell immune response through glycans in inflammatory bowel disease.
  4. Study indicates that Mgat5 genotype affects both behaviour and physical outcomes in response to early life stress, suggesting some shared pathways for both in a mouse model. These results provide a starting point for studying the mechanisms by which protein N-glycosylation mediates the effects of early life adversity.
    Title: Mgat5 modulates the effect of early life stress on adult behavior and physical health in mice.
  5. Overexpression of GnT-V exacerbated murine experimental colitis by inducing macrophage dysfunction, thereby enhancing colorectal tumorigenesis
    Title: N-Acetylglucosaminyltransferase V exacerbates murine colitis with macrophage dysfunction and enhances colitic tumorigenesis.
  6. Expression of GnT-V was also elevated in bleomycin (BLM)-injected sclerotic skin, and MGAT5(-/-) mice were resistant to BLM-induced skin sclerosis with reduced collagen type 1 alpha1 content.
    Title: Oligosaccharide modification by N-acetylglucosaminyltransferase-V in macrophages are involved in pathogenesis of bleomycin-induced scleroderma.
  7. GnT-V regulates the canonical Wnt/beta-catenin signaling pathway by modulating N-glycosylation of Wnt receptors, which changes cancer stem cells in colorectal tumors, causing altered colon tumorigenesis and adenoma progression in Apc(min/+) mice
    Title: Post-translational glycoprotein modifications regulate colon cancer stem cells and colon adenoma progression in Apc(min/+) mice through altered Wnt receptor signaling.
  8. this study suggests that the GnT-V expression is inversely correlated with radiation sensitivity in prostate cancer(PCa)cells
    Title: Inhibition of N-acetylglucosaminyltransferase V enhances sensitivity of radiotherapy in human prostate cancer.
  9. The GnT-V prevented steatohepatitis progression through modulating lymphocyte and HSC functions.
    Title: N-Acetylglucosaminyltransferase V regulates TGF-β response in hepatic stellate cells and the progression of steatohepatitis.
  10. GnT-V overexpression promotes EMT and keratinocyte migration in part through enhanced EGF receptor signaling.
    Title: Enhanced epithelial-mesenchymal transition-like phenotype in N-acetylglucosaminyltransferase V transgenic mouse skin promotes wound healing.
Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (1)  1 citation
  • Endonuclease-mediated (3) 

Pathways from PubChem

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General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables acetylglucosaminyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity ISO
Inferred from Sequence Orthology
more info
  
enables glycosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables manganese ion binding ISO
Inferred from Sequence Orthology
more info
  
enables protein phosphatase inhibitor activity ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in negative regulation of protein tyrosine phosphatase activity ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of cell migration ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of receptor signaling pathway via STAT ISO
Inferred from Sequence Orthology
more info
  
involved_in protein N-linked glycosylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within protein N-linked glycosylation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within protein N-linked glycosylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein N-linked glycosylation ISO
Inferred from Sequence Orthology
more info
  
involved_in protein N-linked glycosylation via asparagine ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in Golgi apparatus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in Golgi apparatus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extracellular region IEA
Inferred from Electronic Annotation
more info
  
located_in membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Names
GNT-V
N-acetylglucosaminyl-transferase V
alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
beta1,6N-acetylglucosaminyltransferase V
glcNAc-T V
NP_660110.2
XP_006529118.1
XP_006529119.1
XP_006529120.1
XP_030110888.1
XP_036022125.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_145128.3 → NP_660110.2  alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

    See identical proteins and their annotated locations for NP_660110.2

    Status: VALIDATED

    Source sequence(s)
    AK030402, BC125533, BY752687
    Consensus CDS
    CCDS15245.1
    UniProtKB/Swiss-Prot
    Q8R4G6
    UniProtKB/TrEMBL
    Q059T5
    Related
    ENSMUSP00000038359.5, ENSMUST00000038361.11
    Conserved Domains (2) summary
    pfam15027
    Location:7 → 137
    DUF4525; Domain of unknown function (DUF4525)
    pfam15024
    Location:171 → 725
    Glyco_transf_18; Glycosyltransferase family 18

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    127132450..127413760
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006529056.5 → XP_006529119.1  alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

    See identical proteins and their annotated locations for XP_006529119.1

    UniProtKB/Swiss-Prot
    Q8R4G6
    UniProtKB/TrEMBL
    Q059T5
    Conserved Domains (2) summary
    pfam15027
    Location:7 → 137
    DUF4525; Domain of unknown function (DUF4525)
    pfam15024
    Location:171 → 725
    Glyco_transf_18; Glycosyltransferase family 18

  2. XM_006529055.5 → XP_006529118.1  alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

    See identical proteins and their annotated locations for XP_006529118.1

    UniProtKB/Swiss-Prot
    Q8R4G6
    UniProtKB/TrEMBL
    Q059T5
    Conserved Domains (2) summary
    pfam15027
    Location:7 → 137
    DUF4525; Domain of unknown function (DUF4525)
    pfam15024
    Location:171 → 725
    Glyco_transf_18; Glycosyltransferase family 18

  3. XM_030255028.2 → XP_030110888.1  alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

    UniProtKB/Swiss-Prot
    Q8R4G6
    UniProtKB/TrEMBL
    Q059T5
    Conserved Domains (2) summary
    pfam15027
    Location:7 → 137
    DUF4525; Domain of unknown function (DUF4525)
    pfam15024
    Location:171 → 725
    Glyco_transf_18; Glycosyltransferase family 18

  4. XM_006529057.5 → XP_006529120.1  alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

    See identical proteins and their annotated locations for XP_006529120.1

    UniProtKB/Swiss-Prot
    Q8R4G6
    UniProtKB/TrEMBL
    Q059T5
    Conserved Domains (2) summary
    pfam15027
    Location:7 → 137
    DUF4525; Domain of unknown function (DUF4525)
    pfam15024
    Location:171 → 725
    Glyco_transf_18; Glycosyltransferase family 18

  5. XM_036166232.1 → XP_036022125.1  alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A isoform X1

    UniProtKB/Swiss-Prot
    Q8R4G6
    UniProtKB/TrEMBL
    Q059T5
    Related
    ENSMUSP00000129166.2, ENSMUST00000171405.2
    Conserved Domains (2) summary
    pfam15027
    Location:7 → 137
    DUF4525; Domain of unknown function (DUF4525)
    pfam15024
    Location:171 → 725
    Glyco_transf_18; Glycosyltransferase family 18
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8R4G6.1 GenPept UniProtKB/Swiss-Prot:Q8R4G6

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