GEO DataSets

Monitoring of mRNA splice variants for more than 10,000 multi-exon genes using arrays with oligonucleotide probes positioned at exon-exon junctions. mRNA from 52 tissues and cell lines examined. Results provide tissue distribution of splice variants and identify novel splice variants.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 51 tissue sets

Platform:

GPL546

Series:

GSE740

52 Samples

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(Submitter supplied) This series represents 52 tissues hybridized across 5 different chip patterns. Probes were placed at every exon-exon junction in each transcript. Keywords = junction alternate splicing oligonucleotide Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS829 GDS830 GDS831 GDS832 GDS833

5 related Platforms

264 Samples

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Monitoring of mRNA splice variants for more than 10,000 multi-exon genes using arrays with oligonucleotide probes positioned at exon-exon junctions. mRNA from 52 tissues and cell lines examined. Results provide tissue distribution of splice variants and identify novel splice variants.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 53 tissue sets

Platform:

GPL547

Series:

GSE740

54 Samples

Download data

Monitoring of mRNA splice variants for more than 10,000 multi-exon genes using arrays with oligonucleotide probes positioned at exon-exon junctions. mRNA from 52 tissues and cell lines examined. Results provide tissue distribution of splice variants and identify novel splice variants.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 53 tissue sets

Platform:

GPL545

Series:

GSE740

54 Samples

Download data

Monitoring of mRNA splice variants for more than 10,000 multi-exon genes using arrays with oligonucleotide probes positioned at exon-exon junctions. mRNA from 52 tissues and cell lines examined. Results provide tissue distribution of splice variants and identify novel splice variants.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 49 tissue sets

Platform:

GPL544

Series:

GSE740

50 Samples

Download data

Monitoring of mRNA splice variants for more than 10,000 multi-exon genes using arrays with oligonucleotide probes positioned at exon-exon junctions. mRNA from 52 tissues and cell lines examined. Results provide tissue distribution of splice variants and identify novel splice variants.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 53 tissue sets

Platform:

GPL543

Series:

GSE740

54 Samples

Download data

(Submitter supplied) Alternative pre-mRNA splicing increases proteomic diversity and provides a potential mechanism underlying both phenotypic diversity and susceptibility to genetic disorders in human populations. To investigate the variation in splicing among humans on a genomewide scale, we use a comprehensive exon-targeted microarray to examine alternative splicing in lymphoblasts derived from the CEPH HapMap population. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

49 Samples

Download data: CEL, TXT

(Submitter supplied) Manuscript Title: Discovery of tissue-specific exons using comprehensive human exon microarrays We have designed a high-density oligonucleotide microarray with probesets for more than one million annotated and predicted exons in the human genome. Using these arrays and a simple algorithm that normalizes exon signal to signal from the gene as a whole, we have identified tissue-specific exons from a panel of 16 different normal adult tissues. more...

Organism:

Homo sapiens

Type:

Expression profiling by genome tiling array

Platform:

GPL4253

48 Samples

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(Submitter supplied) Alternative splicing of pre-mRNA generates protein diversity and has been linked to cancer progression and drug response. Exon microarray technology enables genome-wide quantication of expression levels for the majority of exons and facilitates the discovery of alternative splicing events. Analysis of exon array data is more challenging than gene expression data and there is a need for reliable quantication of exons and alternative spliced variants. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5188

15 Samples

Download data: CEL

(Submitter supplied) Background Alternative splicing is known to increase the complexity of mammalian transcriptomes since nearly all mammalian genes express multiple pre-mRNA isoforms. However, our knowledge of the extent and function of alternative splicing in early embryonic development is based mainly on a few isolated examples. High throughput technologies now allow us to study genome-wide alternative splicing during mouse development. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6096 GPL6193

50 Samples

Download data: CEL

(Submitter supplied) Differentiating erythroid cells execute a unique gene expression program that insures synthesis of the appropriate proteome at each stage of maturation. Standard expression microarrays provide important insight into erythroid gene expression, but cannot detect qualitative changes in transcript structure, mediated by RNA processing, that alter structure and function of encoded proteins. We analyzed stage-specific changes in the late erythroid transcriptome via use of high resolution microarrays that detect altered expression of individual exons. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL5188 GPL8119

17 Samples

Download data: CEL

(Submitter supplied) We showed that the over-expression of the SR protein SRSF2 led to the regulation of transcript abundance of many genes.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

12 Samples

Download data: TXT

(Submitter supplied) Alternative splicing of pre-mRNA is a mechanism that increases the protein diversity of a single gene by differential exon inclusion during post-transcriptional processing. While alternative splicing is established to occur during lymphocyte activation, little is known about the role it plays during the immune response. Our study is among the first reports of a systematic genome-wide analysis using whole exon DNA microarrays integrating alternative splicing and differential gene expression. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5188

80 Samples

Download data: CEL

(Submitter supplied) In order to compare previously generated transcriptome data by second generation sequencing (Sultan et al. Science 2008), we interrogated the human Affymetrix exon chips 1.0ST using the same source of material.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7557

2 Samples

Download data: CEL

(Submitter supplied) Transcriptome of HEK and B cells were analyzed by microarray and RNA-Seq parallely. Both platforms were then compared in terms of sensitivity. To assess whether values were a reliable indicator of gene activity, we correlated these values with hypophosphorylated RNA polymerase II (PolIIa) occupancy, used as a landmark of transcription initiation. For HEK, we identified PolIIa islands by chromatin immunoprecipitation and sequencing (ChIP-Seq).

Organism:

Homo sapiens

Type:

Expression profiling by array; Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL6104 GPL9052

11 Samples

Download data: TXT

(Submitter supplied) Alternative pre-messenger RNA splicing impacts development, physiology, and disease, but its regulation in humans is not well understood, partially due to the limited scale to which the expression of specific splicing events has been measured. We generated the first genome-scale expression compendium of human alternative splicing events using custom whole-transcript microarrays monitoring expression of 24,426 mutually exclusive alternative splicing event pairs in 48 diverse human samples. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

17 related Platforms

813 Samples

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(Submitter supplied) Background: The vast majority of human genes (.70%) are alternatively spliced. Although alternative pre-mRNA processing is modified in multiple tumors, alternative hyper-splicing signatures specific to particular tumor types are still lacking. Here, we report the use of Affymetrix Human Exon Arrays to spot hyper-splicing events characteristic of myasthenia gravis (MG)-thymoma, thymic tumors which develop in patients with MG and discriminate them from colon cancer changes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL5188 GPL5175

8 Samples

Download data: CEL, TXT

(Submitter supplied) In Alternative Expression Analysis (ALEXA)-Seq we developed a method to analyze massively parallel RNA sequence data to catalogue transcripts and assess differential and alternative expression of known and predicted messenger RNA (mRNA) isoforms in cells and tissues. As proof-of-principle, we applied the approach to a comparison of fluorouracil responsive and non-responsive human colorectal cancer cell lines. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9115

2 Samples

Download data: TXT

(Submitter supplied) Alternative mRNA splicing represents an effective mechanism of regulating gene function and is a key element to increase the coding capacity of the human genome. Today, an increasing number of reports illustrates that aberrant splicing events are common and functionally important for cancer development. However, more comprehensive analyses are warranted to get novel insights into the biology underlying malignancies like e.g. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5188

64 Samples

Download data: CEL

(Submitter supplied) The human Genome-Wide SpliceArray (GWSA), a novel microarray platform, utilizes an existing probe design concept to monitor such transcript diversity on a genome scale. The human GWSA allows the detection of alternatively spliced events within the human genome through the use of exon body and junction probes to provide a direct measure of each transcript, through simple calculations derived from expression data. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7884

12 Samples

Download data: CEL