GEO DataSets

Analysis of tumor progression locus 2 (Tpl2)-deficient bone marrow derived macrophages (BMDMs) stimulated with lipopolysaccharide (LPS) for 4 hrs. Tpl2 (Map3k8) is a serine/threonine kinase critical in innate immunity. Results provide insight into the role of Tpl2 in BMDMs during acute inflammation.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 genotype/variation, 2 protocol sets

Platform:

GPL1261

Series:

GSE48338

8 Samples

Download data: CEL

(Submitter supplied) In autoimmune diseases, accumulation of activated leukocytes correlates with inflammation and disease progression, and therefore, disruption of leukocyte trafficking is an active area of research. The protein kinase Tpl2 (MAP3K8) regulates leukocyte inflammatory responses and is also being investigated for therapeutic inhibition during autoimmunity. Herein, we addressed the contribution of Tpl2 to the regulation of macrophage chemokine and chemokine receptor expression and subsequent migration in vivo using a mouse model of Tpl2 ablation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5385

Platform:

GPL1261

8 Samples

Download data: CEL

(Submitter supplied) We perform CRISPR knockout of IRAK4 usig two different sgRNAs in murine KP2 cells, and then reexpressed murine IRAK4 in these two KO cell lines. We performed RNAseq on wild-type, IRAK4 KO and IRAK4 KO/rescue cell lines to investigate pathways contolled by IRAK4.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: TXT

(Submitter supplied) The cytokine interleukin-12 (IL-12) is known to play a central role in adaptive and innate immunity. We employed microarray analysis of IL-12 induced gene expression to provide further insights into its effects on immune response. Keywords: Paired study of expression levels: Treated vs. Control

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS3497 GDS3498

Platforms:

GPL97 GPL96

28 Samples

Download data: CEL

Analysis of cultured peripheral blood mononuclear cells (PBMCs) stimulated with interleukin-12 (IL-12). PBMCs obtained from 7 donors. IL-12 plays a central role in adaptive and innate immunity. Results provide further insight into the effects of IL-12 on the immune response.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 7 individual sets

Platform:

GPL97

Series:

GSE12839

14 Samples

Download data: CEL

Analysis of cultured peripheral blood mononuclear cells (PBMCs) stimulated with interleukin-12 (IL-12). PBMCs obtained from 7 donors. IL-12 plays a central role in adaptive and innate immunity. Results provide further insight into the effects of IL-12 on the immune response.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 7 individual sets

Platform:

GPL96

Series:

GSE12839

14 Samples

Download data: CEL

(Submitter supplied) Tumor derived factors can promote the differentiation of hematopoietic precursors into suppressive myeloid derived suppressor cells however, the molecular pathways that regulate this differentiation are not fully disclosed. In this study we identified a pathway by which tumor derived factors induce the expression of CCL3 and CCL4 from early HSPCs that autocrinally bind to CCR1 and CCR5 mediate the differentiation of BM cells into suppressive and protumoral cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL23092

9 Samples

Download data: CEL

(Submitter supplied) We conducted gene expression profile analysis by using WT and Tpl2-deficient hepatocytes with IL-25 treatment.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

2 Samples

Download data: TXT

(Submitter supplied) Bone marrow derived macrophages (BMDM) generated from c57bl/6j mice bone marrow cells were stimulated for 18 h with 12 microgram/ml adiponectin, RNA from non-stimulated or 18 h adiponectin-stimulated BMDM subjected to a agilent microarray analysis

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

3 Samples

Download data: TXT

(Submitter supplied) Severe fever with thrombocytopenia syndrome phlebovirus (SFTSV), listed in the WHO most dangerous pathogens, has 12-30% fatality rates with a characteristic thrombocytopenia syndrome. With a majority of clinically diagnosed SFTSV patients older than ~50 years, age is a critical risk factor for SFTSV morbidity and mortality. Here, we report an age-dependent ferret model of SFTSV infection and pathogenesis that fully recapitulates the clinical manifestations of human infections. more...

Organism:

Mustela putorius furo

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25735

13 Samples

Download data: XLS, XLSX

(Submitter supplied) TPL2 (MAP3K8) is a central signaling node in the inflammatory response of peripheral immune cells.  We find that TPL2 kinase activity modulates microglial cytokine release and is required for microglia-mediated neuron death in vitro.  In acute in vivo neuroinflammation settings, TPL2 kinase activity regulates cytokine levels and activation states of microglia.  In a tauopathy model of chronic neurodegeneration, loss of TPL2 kinase activity reduces neuroinflammation and rescues synapse loss, brain volume loss, and behavioral deficits.  Single-cell RNAseq analysis indicates protection in the tauopathy model was associated with reductions in activated microglia subpopulations as well as infiltrating peripheral immune cells.  Overall, using various models, we find that TPL2 kinase activity promotes multiple harmful consequences of microglial activation in the brain including cytokine release, iNOS induction, astrocyte activation, and immune cell infiltration.  Consequently, inhibiting TPL2 kinase activity could represent a potential therapeutic strategy in neurodegenerative conditions.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

18 Samples

Download data: RDATA

(Submitter supplied) Gene expression changes in metastasis associated macrophage (MAM) FACS sorted cells from mice bearing pulmonary metastasis of breast tumor cells were compared with lung and spleen resident macrophages sorted from healthy mice using same sorting protocol.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13179

12 Samples

Download data: PAIR

(Submitter supplied) We used mouse Clariom-S microarrays to study the gene expression profile of ID8 cancer cells stimulated with C-C Chemokine Ligand 6 (CCL6).

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL23038

6 Samples

Download data: CEL

(Submitter supplied) The omentum is the most common site of ovarian cancer metastasis. Immune cell clusters called milky spots are found throughout the omentum. It is however unknown if these immune cells contribute to ovarian cancer metastasis. Here we report that omental macrophages promote the migration and colonization of ovarian cancer cells to the omentum through the secretion of chemokine ligands that interact with chemokine receptor 1 (CCR1). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: TXT

(Submitter supplied) Identification of novel pathways in the development of atherosclerosis. Here, we are looking at changes in gene expression that occur in the aorta with the development of atherosclerosis

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL19727

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6096 GPL16570 GPL1261

33 Samples

Download data: CEL

(Submitter supplied) In wild-type mice, expression of chemokines that are ligands for Ccr2, Cxcr3, and Cxcr2 increased at days 32 to 41 post-infection, with a return to baseline by day 75. Concomitant increases were seen in Ccr2 and Cxcr3 but not in Cxcr2 expression. Induction of these same factors also occurred in CD40-ligand and CD40 knockout mice but only at a much later time-point, during uncontrolled Pneumocystis pneumonia (PCP). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6096

12 Samples

Download data: CEL

(Submitter supplied) In wild-type mice, expression of chemokines that are ligands for Ccr2, Cxcr3, and Cxcr2 increased at days 32 to 41 post-infection, with a return to baseline by day 75. Concomitant increases were seen in Ccr2 and Cxcr3 but not in Cxcr2 expression. Induction of these same factors also occurred in CD40-ligand and CD40 knockout mice but only at a much later time-point, during uncontrolled Pneumocystis pneumonia (PCP). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

5 Samples

Download data: CEL

(Submitter supplied) For both CD4 and micropajfe cell types, upregulated genes were primarily related to immune activation and proliferation, while down-regulated genes represented more diverse processes, including cell membranes, vasculature development, cell adhesion, and lipid-related metabolic processes.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

8 Samples

Download data: CEL

(Submitter supplied) For both CD4 and micropajfe cell types, upregulated genes were primarily related to immune activation and proliferation, while down-regulated genes represented more diverse processes, including cell membranes, vasculature development, cell adhesion, and lipid-related metabolic processes.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

8 Samples

Download data: CEL