GEO DataSets

Analysis of sorted FBC (F4/80+ CD11b+ CD11c+) cells and FB (F4/80+ CD11b+ CD11c-) perigonadal adipose tissue macrophages (ATM) from lean (C57BL/6J Lep +/+) and obese (C57BL/6J Lep ob/ob) mice. Results provide insight into molecular mechanisms underlying obesity-associated lipid metabolism in ATMs.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 cell type, 2 strain sets

Platform:

GPL1261

Series:

GSE53403

16 Samples

Download data: CEL

(Submitter supplied) In mammals, expansion of adipose tissue mass induces accumulation of adipose tissue macrophages (ATMs). We isolated CD11c- (FB) and CD11c+ (FBC) perigonadal ATMs from SVCs of lean (C57BL/6J Lep +/+) and obese leptin-deficient (C57BL/6J Lep ob/ob) mice. We used expression microarrays to generate transcription profiles of perigonadal ATMs from lean (C57BL/6J Lep +/+) and obese (C57BL/6J Lep ob/ob) mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5209

Platform:

GPL1261

16 Samples

Download data: CEL

(Submitter supplied) Obesity-associated insulin resistance is characterized by a state of chronic, low-grade inflammation that is associated with the accumulation of M1 proinflammatory macrophages in adipose tissue. Although different evidence explains the mechanisms linking the expansion of adipose tissue and adipose tissue macrophage (ATM) polarization, in the current study we investigated the concept of lipid-induced toxicity as the pathogenic link that could explain the trigger of this response. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

15 Samples

Download data: CEL

(Submitter supplied) Recent studies have identified intracellular metabolism as a fundamental determinant of macrophage function. In obesity, proinflammatory macrophages accumulate in adipose tissue and trigger chronic low-grade inflammation, that promotes the development of systemic insulin resistance, yet changes in their intracellular energy metabolism are currently unknown. We therefore set out to study metabolic signatures of adipose tissue macrophages (ATMs) in lean and obese conditions. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11533

8 Samples

Download data: CEL

(Submitter supplied) Macrophage-mediated inflammatory response has been implicated in the pathogenesis of obesity and insulin resistance. Brd4 has emerged as a key regulator in the innate immune response. However, the role of Brd4 in obesity-associated inflammation and insulin resistance remains uncharacterized. Here, we demonstrated that myeloid-lineage specific Brd4 knockout (Brd4-CKO) mice were protected from high-fat-diet (HFD)-induced obesity with less fat accumulation, higher energy expenditure, and increased lipolysis in adipose tissue. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23479

6 Samples

Download data: TXT

(Submitter supplied) We used MeDIP-Seq to screen for genes that may be regulated by DNA methylation in macrophages isolated from adipose tissue of lean and obese mice.

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL19057

2 Samples

Download data: WIG

(Submitter supplied) We used microRNA microarrays to identify dysregulated microRNAs in macrophages isolated from adipose tissue of lean and obese mice.

Organism:

Mus musculus; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL21572

6 Samples

Download data: CEL, CHP

(Submitter supplied) We used transcriptome microarrays to identify gene expression changes in macrophages isolated from adipose tissue of lean and obese mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

6 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL19057 GPL13112

70 Samples

Download data

(Submitter supplied) We report that obese fat tissue of mice contain multiple distinct populations of adipose tissue macrophage (ATM) with unique transcriptomes and chromatin landscapes. Mouse Ly6c ATMs express genes that are adipogenic, while CD9 ATMs express pro-inflammatory genes under the control of activating transcription factors. Adoptive transfer of Ly6c ATMs into lean mice activates gene programs typical of normal adipocyte physiology. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL13112 GPL19057

27 Samples

Download data: TXT

(Submitter supplied) We report that obese fat tissue of mice contain multiple distinct populations of adipose tissue macrophage (ATM) with unique transcriptomes and chromatin landscapes. Mouse Ly6c ATMs express genes that are adipogenic, while CD9 ATMs express pro-inflammatory genes under the control of activating transcription factors. Adoptive transfer of Ly6c ATMs into lean mice activates gene programs typical of normal adipocyte physiology. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

6 Samples

Download data: BW

(Submitter supplied) We report that obese fat tissue of mice contain multiple distinct populations of adipose tissue macrophage (ATM) with unique transcriptomes and chromatin landscapes. Mouse Ly6c ATMs express genes that are adipogenic, while CD9 ATMs express pro-inflammatory genes under the control of activating transcription factors. Adoptive transfer of Ly6c ATMs into lean mice activates gene programs typical of normal adipocyte physiology. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

37 Samples

Download data: TXT

(Submitter supplied) Investigation of whole genome gene expression level changes in GW501516 (a Ppard ligand) or Il-4 treated Ppard-overexpressing RAW 264.7 macrophages as compared to vehicle. Alternative activation of adipose tissue resident macrophages inhibits obesity-induced metabolic inflammation. In the current study we profile genes regulated by two M2 inducers, Il-4 or Ppard agonists and find that alternative activation promotes the cell survival program, while inhibiting that of inflammation-related cell death.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10192

9 Samples

Download data: PAIR

(Submitter supplied) We profiled three prominent ATM subtypes from human visceral omental adipose tissue in obesity by RNA-seq. In the related manuscript, we evaluated differences in their signatures and their relationship to type 2 diabetes: Visceral (VAT) and subcutaneous (SAT) adipose tissue samples were collected from diabetic and non-diabetic obese subjects to evaluate cellular content and gene expression. VAT CD206+CD11c− ATMs were increased in diabetic subjects, scavenger receptor-rich with low intracellular lipids, secreted proinflammatory cytokines, and diverged significantly from two CD11c+ ATM subtypes, which were lipid-laden, lipid antigen presenting, and overlapped with monocyte signatures. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

9 Samples

Download data: TXT

(Submitter supplied) Adipose tissue stromal cells contribute to the regulation of adipose tissue in lean and obese states. Myeloid cells such as adipose tissue macrophages (ATMs) and dendritic cells (ATDCs) undergo both quantitative and qualitative changes with obesity. Due to similarity in markers the identify of adipose tissue dendritic cells and macrophages has been elusive. We have refined prior protocols to unambiguously discern ATM and ATDC in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17400

24 Samples

Download data: CEL

(Submitter supplied) Using t-distributed stochastic neighbor embedding (t-SNE) analysis, we identified 23 transcriptionally distinct cell clusters which are present in the eWAT when the four experimental groups were combined (ND and HFD with AMTs or without).Our analysis identifies six main cell clusters of CD45- cells: two adipocyte stem cell fractions (ASC1 and ASC2), stromal cells, two fractions of vascular endothelial cells (VEC1 and VEC2) as well as a population showing a gene signature for of mesothelial cells, among the CD45+ cells, seventeen lymphoid and myeloid cell subpopulations are detectable in the eWAT ranging from monocytes, monocyte-derived macrophages, dendritic cells, eosinophils, mast cells, B cells, CD4+ Th2, CD4+ Th17, Tregs and CD8+ T cells, NK cells, NKT cells In obese mice, one week absence of ATM in the eWAT affected mostly the non-haematopoetic cell compartment, while the total number of cell clusters and percentage of haematopoetic CD45+ cells were maintained roughtly the same between the four experimental groups, whereas ablation of ATMs in lean mice did not perturb dramatically the cluster distribution of CD45- cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

32 Samples

Download data: CSV, MTX, TSV

(Submitter supplied) Three different macrophage subsets and one subpopulation of monocyte derived cells were sorted from lean and obese mice adipose tissue

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

21 Samples

Download data: TXT

(Submitter supplied) Sucnr1 signaling promotes M2 polarization

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11180

16 Samples

Download data: CEL

(Submitter supplied) Obesity drives significant changes in adipose tissue that precede development of tissue and systemic insulin resistance. Immune cell infiltration and inflammation are known contributors to these changes but there is limited understanding of their spatial context tissue-wide. We sought to identify spatial patterning in epididymal adipose tissue immune cells in a time course of diet-induced obesity in mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

7 Samples

Download data: CSV, H5

(Submitter supplied) Dyslipidemia is a main driver of cardiovascular diseases. The ability of macrophages to scavenge excess lipids implicate them as mediators in this process and understanding the mechanisms underlying macrophage lipid metabolism is key to the development of new treatments. Here we investigated how adipose tissue macrophages (ATMs) regulate post-prandial cholesterol transport. Single-cell RNA sequencing demonstrated that ingestion of lipids led to specific transcriptional activation of a population of resident ATMs expressing Lyve1, Tim4 and ABCA1.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

2 Samples

Download data: MTX, TSV