GEO DataSets

Analysis of hypoxic A549 cells treated with oligooxopiperazines (OOPs) BB2-125, BB2-162, and BB2-282. OOPs are designed mimics of a key α-helical domain at the interface of hypoxia-inducible factor 1α (HIF1α) and coactivator p300. Results provide insight into the effect of OOPs on HIF signaling.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 agent, 2 stress sets

Platform:

GPL6244

Series:

GSE48134

15 Samples

Download data: CEL

(Submitter supplied) We performed gene expression profiling of oligooxopiperazines (OPs) targeting the hypoxia-inducible transcription factor complex. Treatment of cells with OPs inhibited hypoxia-inducible gene expression in A549 cells.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5067

Platform:

GPL6244

15 Samples

Download data: CEL

(Submitter supplied) We performed gene expression profiling of hydrogen-bond surrogate that targets hypoxia-inducible transcription factior complex and results in inhibition of hypoxia-inducible genes with relatively minimal perturbation of non-targeted signaling pathways.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

12 Samples

Download data: CEL

(Submitter supplied) The C-terminal activation domain (C-TAD) of the hypoxia-inducible transcription factors HIF-1? and HIF-2? binds the CH1 domains of the related transcriptional coactivators CREB-binding protein (CBP) and p300, an oxygen-regulated interaction thought to be highly essential for hypoxia-responsive transcription. The role of the CH1 domain in vivo is unknown, however. We created mutant mice bearing deletions in the CH1 domains (?CH1) of CBP and p300 that abrogate their interactions with the C-TAD, revealing that the CH1 domains of CBP and p300 are genetically non-redundant and indispensable for C-TAD transactivation function. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1261 GPL339

32 Samples

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(Submitter supplied) The CH1 protein interaction domain of the transcriptional coactivators p300 and CBP is thought to interact with HIF-1alpha and this interaction is thought to be critical to the expression of HIF-1alpha target genes in response to hypoxia. Trichostatin A (TSA), an inhibitor of histone deacetylases, has been reported to repress the expression of HIF-1alpha target genes. To test the requirement of the CH1 domain and TSA for gene expression in response to dipyridyl (a hypoxia mimetic), primary mouse embryonic fibroblasts (MEFs) were generated from C57Bl/6x129/Sv F2 e14.5 embryos that contain a deletion in the CH1 domain of three of four alleles of CBP and p300. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2162

Platform:

GPL1261

16 Samples

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(Submitter supplied) The CH1 protein interaction domain of the transcriptional coactivators p300 and CBP is thought to interact with HIF-1alpha and this interaction is thought to be critical to the expression of HIF-1alpha target genes in response to hypoxia. To test the requirement of the CH1 domain for gene expression in response to hypoxia, rimary mouse embryonic fibroblasts (MEFs) were generated from C57Bl/6x129/Sv F2 e14.5 embryos that contain a deletion in the CH1 domain of three of four alleles of CBP and p300. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2161

Platform:

GPL1261

12 Samples

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(Submitter supplied) The CH1 (TAZ) domain of the transcriptional coactivators p300 and CBP has been reported to interact with the transcription factor HIF-1alpha and this interaction is thought to be critical for HIF-1alpha target gene expression in response to hypoxia. To determine the requirement for the CH1 domain in hypoxia-responsive gene expression, primary mouse embryonic fibroblasts (MEFs) were generated from e14.5 C57B/6x129/Sv F2 embryos that were either wildtype or bore deletion mutations in the CH1 protein binding domains of both alleles of p300 and one allele of CBP (tri_CH1). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2160

Platform:

GPL339

4 Samples

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Analysis of trichostatin A (TSA)-treated hypoxic fibroblasts with deletions in CH1 domains of p300 and CBP, and a p300 or CBP allele knockout. TSA is a histone deacetylase inhibitor. Results provide insight into the role of deacetylase activity in CH1-independent hypoxia inducible transcription.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 agent, 4 genotype/variation sets

Platform:

GPL1261

Series:

GSE3296

16 Samples

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Analysis of hypoxic fibroblasts with deletions in the CH1 domains of p300 and CBP combined with a p300 or CBP allele knockout. CH1 domains interact with the C-TAD domain of hypoxia-inducible transcription factors HIF-1a and -2a. Results provide insight into the role for CH1 domains in hypoxia.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 genotype/variation, 2 stress sets

Platform:

GPL1261

Series:

GSE3196

12 Samples

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Analysis of hypoxic fibroblasts bearing deletions in the CH1 domains of 1 allele of p300 and 2 alleles of CBP. CH1 domains interact with the C-TAD domain of hypoxia-inducible transcription factors HIF-1a and -2a. Results provide insight into the role of CH1 domains in the hypoxia response.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 stress sets

Platform:

GPL339

Series:

GSE3195

4 Samples

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(Submitter supplied) The activation of the transcription factor Hypoxia-inducible factor-1 (HIF-1) plays an essential role in tumor development, tumor progression and resistance to chemo- and radiotherapy. In order to identify compounds targeting the HIF pathway, a small-molecule library was screened using a luciferase-driven HIF-1 reporter cell line under hypoxia. The high throughput screen led to the identification of a class of aminoalkyl-substituted compounds that inhibited hypoxia-induced HIF-1 target gene expression in human lung cancer cell lines at low nanomolar concentrations but did not affect expression levels of genes outside of the HIF-1 pathway. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

6 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of human PC-3 prostate cancer cells lentivirally infected with non-target control (NTC) short hairpin (sh)RNA comparing with lentivirally shRNA mediated human ETV4 knock-down. Cells were either cultured for 24 hours at 20% oxygen tension or 0.2% oxygen. Goal was to determine (i) genes affected by hypoxia in PC-3 NTC cells and (ii) identification of hypoxically induced genes requiring ETV4 for hypoxic regulation.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13607

6 Samples

Download data: TXT