GEO DataSets

Analysis of postmortem midbrain specimens from individuals who died from cocaine abuse. Midbrain dopamine-synthesizing neurons play a important role in the addiction process. Results provide insight into the molecular pathophysiological changes in the midbrain associated with cocaine abuse.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 20 individual sets

Platform:

GPL6947

Series:

GSE54839

60 Samples

Download data: IDAT

(Submitter supplied) Midbrain dopamine (DA)-synthesizing neurons play a key role in the addiction process, providing a compelling rationale for determining drug-induced molecular changes arising in these cells. This microarray-based study determined the profiles of midbrain gene expression in chronic cocaine abusers (n = 10) and well-matched drug-free control subjects (n = 10). Array-related procedures were performed in triplicate for each subject. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5047

Platform:

GPL6947

60 Samples

Download data: IDAT, TXT

(Submitter supplied) Maintenance of the drug-addicted state involves changes in gene expression in different neuronal cell types and neural circuits. Midbrain dopamine neurons in particular mediate numerous responses to drugs of abuse. Long noncoding RNAs (lncRNAs) regulate CNS gene expression through a variety of mechanisms, but very little is known about their role in drug abuse. The proportion of lncRNAs that are primate-specific provides a strong rationale for their study in human drug abusers. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL19946

88 Samples

Download data: TXT

(Submitter supplied) Analysis of dopaminergic neuronal gene expression changes by Nurr1 and/or Foxa2 overexpression. Result provides that Foxa2 potentiates Nurr1-induced DA neuronal phenotype gene expression.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

4 Samples

Download data: TXT

(Submitter supplied) Many studies of cocaine-responsive gene expression have focused on changes occurring during cocaine exposure, but few studies have examined the persistence of these changes with cocaine abstinence. Persistent changes in gene expression, as well as alterations induced during abstinence may underlie long-lasting drug craving and relapse liability. Results: Whole-genome expression analysis was conducted on a rat cocaine binge-abstinence model that has previously been demonstrated to engender increased drug seeking and taking with abstinence. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL2896

33 Samples

Download data: TXT

(Submitter supplied) Ablation of the Camk4 gene in dopaminoceptive neurons of the brain was performed using the Cre/loxP system, with the recombinase expressed from a BAC-derived Drd1a promoter. Our goal was to analyze how loss of CaMKIV will affect acitivity-regulated transcription induced by strong stimulation, i.e. cocaine. Keywords: Treatment x Genotype

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

18 Samples

Download data: CEL

(Submitter supplied) total RNA-sequencing on dlPFC neurons from 19 human cocaine addicts (1-19) and 17 healthy controls (21-40)

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

36 Samples

Download data: XLSX

(Submitter supplied) Parkinson's disease (PD) is defined by the degeneration of nigral dopaminergic (DA) neurons and can be caused by monogenic mutations of genes such as parkin. The lack of phenotype in parkin knockout mice suggests that human nigral DA neurons have unique vulnerabilities. Here we generate induced pluripotent stem cells from normal subjects and PD patients with parkin mutations. We demonstrate that loss of parkin in human midbrain DA neurons greatly increases the transcription of monoamine oxidases and oxidative stress, significantly reduces DA uptake and increases spontaneous DA release. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array

Platform:

GPL15098

4 Samples

Download data: TXT