GEO DataSets

Analysis of monocytes stimulated with biologically active forms of vitamin A and vitamin D. Vitamin A plays a role in host defense against Mycobacterium tuberculosis. Results provide insight into molecular mechanisms underlying the antimicrobial activities for vitamin A and vitamin D in monocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 4 individual sets

Platform:

GPL570

Series:

GSE46268

12 Samples

Download data: CEL, CHP

(Submitter supplied) A role for vitamin A in host defense against Mycobacterium tuberculosis has been suggested through epidemiological and in vitro studies; however, the antimicrobial mechanism is unclear. Here, we demonstrate that vitamin A mediates host defense through regulation of cellular cholesterol content. Comparison of monocytes stimulated with all-trans retinoic acid (ATRA) or 1,25-dihydroxyvitamin D3, the biologically active forms of vitamin A and vitamin D respectively, indicates that ATRA and 1,25D3 induce mechanistically distinct antimicrobial activities. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4860

Platform:

GPL570

12 Samples

Download data: CEL, CHP

(Submitter supplied) Tuberculosis remains a major cause of death from an infectious disease worldwide, yet only 10% of people infected with Mycobacterium tuberculosis develop disease. Defining both necessary and sufficient immunologic determinants of protection remains a great scientific challenge. Analysis of peripheral blood gene expression profiles of active tuberculosis patients has identified correlates of risk for disease or pathogenesis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

32 Samples

Download data: CEL

(Submitter supplied) As macrophages are the primary site of Mtb infection and are sites of vitamin D signaling, we have used these cells to understand the molecular mechanisms underlying modulation of the immune response by the hormonal form of vitamin D, 1,25-dihydroxyvitamin D (1,25D). Microarrays were used to measure the changes in gene expression induced by 1,25D treatment of H37Rv-infected THP-1 cells

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4781

Platform:

GPL6244

12 Samples

Download data: CEL, XLSX

Analysis of Mtb H37Rv-infected THP-1 macrophages treated with the hormonal form of vitamin D (1,25D). Macrophages are the primary site of Mtb infection and are sites of 1,25D signaling. Results provide insight into the molecular basis of the 1,25D-enhanced macrophage response to Mtb infection.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 2 infection sets

Platform:

GPL6244

Series:

GSE52819

12 Samples

Download data: CEL

(Submitter supplied) Macrophages are a protective replicative niche for Mycobacterium tuberculosis (Mtb) but can kill the infecting bacterium when appropriately activated. To identify mechanisms of clearance, we compared bacterial restriction by human macrophages after treatment with 26 compounds, including some currently in clinical trials for tuberculosis. All-trans-retinoic acid (ATRA), an active metabolite of vitamin A, drove the greatest increase in Mtb control. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21697

20 Samples

Download data: XLSX

(Submitter supplied) Previous reports have shown low vitamin D serum levels and polymorphisms in the vitamin D receptor (VDR) to be associated with increased risk for TB. Given that 1α,25-dihydroxyvitamin D3 has a role in lipid metabolism control, we tested whether the link between 1α,25-dihydroxyvitamin D3 and tuberculosis involves macrophage lipid metabolism. Since formation of lipid droplets (LD) is a hallmark of lipid dysregulation in M. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

14 Samples

Download data: CEL