GEO DataSets

Analysis of cerebellar neuronal cells for up to 48 hours following treatment with thyroid hormone in vitro. Results provide insight into the role of thyroid hormone in the post-natal development of the cerebellum.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 4 time sets

Platform:

GPL1261

Series:

GSE24793

8 Samples

Download data: CEL

(Submitter supplied) Cerebellar post-natal development is particularly sensitive to thyroid hormone and low levels of thyroid hormone (hypothyroidism) result in permanent defects in cerebellar architecture and function. All cell types of the cerebellum are affected, but the main sign of hypothyroidism in mice is the persistence of the external granular layer, composed of mitotic neuronal precursors at P21. To make the genetic link between thyroid hormone and cerebellar development, we sought to identify new thyroid hormone target genes, in particular in granule cells which represent the vast majority of cerebellar cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4484

Platform:

GPL1261

8 Samples

Download data: CEL

(Submitter supplied) Swiss-Webster B mouse postnatal day 4-5 primary cerebellar culture (pooled from litter mates) treated with sonic hedgehog (Shh), controls (veh), growth arrested (arrest), cycloheximide (cyc) for 1, 3 and 24 hours.

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL76 GPL75

56 Samples

Download data: CEL

(Submitter supplied) A dominant negative allele of TRα1 (TRαAMI) has been introduced only in Sertoli cells using the Cre/lx system and the AmhCre mouse line. Testes were isolated at P3 and RNA was extracted. MRNA were enriched by oligo-dT purification and submitted to Illumina HiSeq2000 sequencing. Reads were aligned on the mouse mm9 genome, gene counts were obtained with the HTSeq-count script and differential expression was investigated with the DESeq2 R package.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

4 Samples

Download data: TXT

(Submitter supplied) Astrocytes mediate the action of thyroid hormone in the brain on other neural cells through the production of the active hormone triiodothyronine (T3) from its precursor thyroxine (T4). T3 has also many effects on the astrocytes in vivo and in culture, but whether these actions are directly mediated by transcriptional regulation is not clear. In this work, we have analyzed the genomic response to T3 of cultured astrocytes isolated from the postnatal mouse cerebral cortex, using RNA sequencing. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

23 Samples

Download data: TXT

(Submitter supplied) Differentiation assays with neural progenitor cells of the enteric nervous system (ENS) showed elongated neurite outgrowth under influence of 3,5,3'-Triiodothyronine (concentrations 50 nm and 100 nm). For analysis, neural cells were stained with TUJ1 (beta-Tubulin III). Microarray analysis should enlighten these results on a genetical basis and give hints about the regulation pathways.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

6 Samples

Download data: CEL

(Submitter supplied) We designed a large scale gene expression study in cerebellar external granular layer in Ts1Cje mice at P0 in order to measure the effects of trisomy 21 on in a enriched cell population (dissected layer) that is affected in Down syndrome in order to correlate gene expression changes to the phenotype observed. Keywords: Down syndrome, Ts1Cje, EGL, hypoplasia

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6105

18 Samples

Download data

(Submitter supplied) We designed a large scale gene expression study in Ts1Cje mice between P0 and P10 in order to measure the effects of trisomy 21 on a large number of samples (56 in total) in a tissue that is affected in Down syndrome (the cerebellum) and to quantify the defect during development in order to correlate gene expression changes to the phenotype observed. Keywords: Down syndrome, Ts1Cje, cerebellum, development, hypoplasia

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4736

28 Samples

Download data: GPR