GEO DataSets

Analysis of undifferentiated human embryonic stem cell line hES-T3 (T3ES), T3ES derived embryoid bodies, and T3ES derived pancreatic islet-like cell clusters (T3pi). Results provide insight into molecular mechanisms involved in the development of pancreas and/or differentiation of islet cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 cell type sets

Platform:

GPL570

Series:

GSE14503

7 Samples

Download data: CEL

(Submitter supplied) Type 1 diabetes is an autoimmune destruction of pancreatic islet beta cell disease, and it is important to find new alternative source of the islet beta cells to replace the damaged cells. Human embryonic stem (hES) cells possess unlimited self-renewal and pluripotency and thus have the potential to provide an unlimited supply of different cell types for tissue replacement. The hES-T3 cells with normal female karyotype were first differentiated into embryoid bodies and then induced to generate the pancreatic islet-like cell clusters, which expressed pancreatic islet cell-specific markers of insulin, glucagon and somatostatin. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4011

Platform:

GPL570

7 Samples

Download data: CEL

(Submitter supplied) Human embryonic stem (hES) cells have the capacities to propagate for extended periods and to differentiate into cell types from all three germ layers both in vitro and in vivo. These characteristics of self-renewal and pluripotency enable hES cells having the potential to provide an unlimited supply of different cell types for tissue replacement, drug screening, and functional genomics studies. The hES-T3 cells with normal female karyotype cultured on either mouse embryonic fibroblasts (MEF) in hES medium (containing 4 ng/ml bFGF) (T3MF) or feeder-free Matrigel in MEF-conditioned medium (supplemented with additional 4 ng/ml bFGF) (T3CM) were found to express very similar profiles of mRNAs and microRNAs, indicating that the unlimited self-renewal and pluripotency of hES cells can be maintained by continuing culture on these two conditions. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL

(Submitter supplied) MicroRNAs (miRNAs) are noncoding RNAs of approximately 22 nucleotides in length that usually suppress the translation of target messenger RNAs (mRNAs) through partial complementarity to the 3¡¦ untranslated region (3¡¦ UTR) of protein-coding mRNAs in animals. However, there is increasing evidence that miRNAs can also reduce the steady-state levels of their target mRNAs in animals. In this investigation, both miRNA and mRNA profiles from the undifferentiated human embryonic stem cell line hES-T3 (T3ES), hES-T3 derived embryoid bodies (T3EB) and hES-T3 differentiated fibroblast-like cells (T3DF) were quantitatively determined. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) Pancreatic islet transplantation as a cure for type 1 diabetes (T1D) cannot be scaled up due to a scarcity of human pancreas donors. In vitro expansion of beta cells from mature human pancreatic islets provides an alternative source of insulin-producing cells. The exact nature of the expanded cells produced by diverse expansion protocols, and their potential for differentiation into functional beta cells, remain elusive. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4027

Platform:

GPL570

33 Samples

Download data: CEL

Analysis of fresh pancreatic islets, expanded islet cells, and re-differentiated cells. Three different protocols were used to expand pancreatic islets, followed by attempts at redifferentiation back to islets. Results provide insight into active pathways in expanded and redifferentiated islets.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 cell type, 4 protocol, 5 time sets

Platform:

GPL570

Series:

GSE15543

33 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Human gammaherpesvirus 8; Rattus norvegicus; Homo sapiens; Macacine gammaherpesvirus 4; Betapolyomavirus macacae

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL7088 GPL7089

38 Samples

Download data: GPR

(Submitter supplied) Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. We aimed to identify the unique signature of miRNAs in human embryonic stem cells. more...

Organism:

Homo sapiens; Mus musculus; Human gammaherpesvirus 8; Betapolyomavirus macacae; Rattus norvegicus; Macacine gammaherpesvirus 4

Type:

Non-coding RNA profiling by array

Platform:

GPL7089

21 Samples

Download data: GPR

(Submitter supplied) Human embryonic stem (hES) cells have unique features: self-renewal ability and pluripotency. They can be continuously cultured in undifferentiated state and give rise to cells and tissues of all three germ layers. Thus hES cells provide a resource not only for cell replacement therapy but also for studying human developmental biology. However, much work needs to be done to understand the molecular mechanisms responsible for the maintenance of the undifferentiatiation state and the differentiation process of human embryonic stem cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7088

17 Samples

Download data: GPR

(Submitter supplied) While enucleation is a critical step in the terminal differentiation of human red blood cells, the molecular mechanisms underlying this unique process remain unclear. To investigate erythroblast enucleation, we studied the erythroid differentiation of human embryonic stem cells (hESCs), which provide a unique model for deeper understanding of the development and differentiation of multiple cell types. more...

Organism:

Mus musculus; Rattus norvegicus; synthetic construct; Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL15235 GPL6480

24 Samples

Download data: TXT

(Submitter supplied) While enucleation is a critical step in the terminal differentiation of human red blood cells, the molecular mechanisms underlying this unique process remain unclear. To investigate erythroblast enucleation, we studied the erythroid differentiation of human embryonic stem cells (hESCs), which provide a unique model for deeper understanding of the development and differentiation of multiple cell types. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

12 Samples

Download data: TXT

(Submitter supplied) While enucleation is a critical step in the terminal differentiation of human red blood cells, the molecular mechanisms underlying this unique process remain unclear. To investigate erythroblast enucleation, we studied the erythroid differentiation of human embryonic stem cells (hESCs), which provide a unique model for deeper understanding of the development and differentiation of multiple cell types. more...

Organism:

Homo sapiens; Mus musculus; Rattus norvegicus; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL15235

12 Samples

Download data: TXT

(Submitter supplied) Genetic studies have identified ≥240 loci associated with type 2 diabetes (T2D), yet most of these loci lie in non-coding regions, masking the underlying molecular mechanisms. Recent studies investigating gene expression in pancreatic islets have provided key insights into the molecular drivers of T2D pathophysiology through comprehensive genetic and genomic analyses. However, similar studies investigating microRNA (miRNA) expression remain limited. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing; Other; Expression profiling by high throughput sequencing

Platforms:

GPL16791 GPL18573

14 Samples

Download data: CSV