GEO DataSets

Analysis of thrombopoietin-cultured CD34+ cells at various time points up to 5 days following treatment with nicotinamide. Thrombopoietin induces CD34+ cells to differentiate into megakaryocytes. Results provide insight into the effect of nicotinamide on megakaryocyte differentiation.

Organism:

Homo sapiens

Type:

Expression profiling by array, log e ratio, 3 agent, 2 individual, 4 time sets

Platform:

GPL887

Series:

GSE4974

28 Samples

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(Submitter supplied) Culture of hematopoietic stem and progenitor cells in the presence of thrombopoietin induces megakaryocytic differentiation. Addition of nicotinamide to thrombopoietin-stimulated cultures results in significant increases in megkaryocytic differentiation including greater polyploidization and enhanced proplatelet formation. This study focuses on understanding the differences in the temporal gene expression pattern during differentiation with and without nicotinamide. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2513

Platform:

GPL887

28 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2926

Platform:

GPL887

77 Samples

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(Submitter supplied) Little is known about the global transcriptional program underlying megakaryocytic (Mk) differentiation, maturation, and apoptosis. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of Mk differentiation human CD34-positive hematopoietic stem and progenitor cells cultured with thrombopoietin, interleukin-3, and Flt3-ligand. The goal this study was to identify genes involved in the various facets of Mk differentiation including commitment, polyploidization, proplatelet formation, and apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL887

36 Samples

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(Submitter supplied) Little is known about the global transcriptional program underlying megakaryocytic (Mk) differentiation, maturation, and apoptosis. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of phorbol-ester-induced Mk differentiation of the megakaryoblastic CHRF-288-11 (CHRF) cell line – a model system for investigating megakaryopoiesis. The goals of this study were to (1) verify the megakaryocytic nature of the CHRF cell line at the transcriptional level, and (2) extract novel insights into the key facets of Mk maturation including polyploidization, proplatelet formation, and apoptosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL887

41 Samples

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Temporal analysis of phorbol ester-treated CHRF-288-11 megakaryoblastic cells induced to undergo megakaryocytic (Mk) differentiation and primary Mk (PriMk) cells derived from cytokine-treated CD34+ peripheral blood cells. Results provide insight into molecular mechanisms underlying megakaryopoiesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, log e ratio, 4 agent, 2 cell line, 13 time sets

Platform:

GPL887

Series:

GSE8914

77 Samples

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(Submitter supplied) Megakaryoblastic Leukemia 1 (MKL1) was identified as part of the t(1;22) translocation specific to acute megakaryoblastic leukemia, but nothing is known regarding its role in hematopoiesis. Here we show that overexpression of MKL1 enhances megakaryocytic differentiation of the Human Erythroleukemia cell line (HEL). Microarray analysis reveals that MKL1 promotes expression of megakaryocyte-specific genes such as glycoprotein V (GP5), as well as cytoskeletal and adhesion molecule genes relevant to megakaryocyte differentiation and proplatelet formation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL570 GPL6104

14 Samples

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