GEO DataSets

Comparison of carotid bodies (CBs) of A/J and DBA/2J animals. The CB is the primary hypoxic chemosensory organ. A/J animals are low responders to hypoxia, while DBA/2J animals are high responders. Results provide insight into the mechanisms regulating hypoxic sensitivity in the CB.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 strain sets

Platform:

GPL1261

Series:

GSE5313

6 Samples

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(Submitter supplied) We hypothesize that gene expression in the CBs of these two strains of mice are divergent thus contributing to the disparity in the phenotypes. More specifically, (1) genes related to CB function are expressed less in the A/J mice compared to DBA/2J mice; (2) gene expression levels of morphogenic and trophic factors of the CB maybe significantly lower in the A/J mice than the DBA/2J mice. In order to test these hypotheses, we utilized microarray analysis to examine transcriptional differences between CBs of both strains of mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2466

Platform:

GPL1261

6 Samples

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(Submitter supplied) Purpose: The DBA/2J mouse is a model for secondary angle-closure glaucoma due to iris atrophy and pigment dispersion, which ultimately leads to increased intraocular pressure (IOP). We sought to correlate changes in retinal gene expression with glaucoma-like pathology by performing microarray analysis of retinal RNA from DBA/2J mice at 3 months before disease onset, and at 8 months, after IOP elevation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1728

Platform:

GPL1261

6 Samples

Download data: CEL

Analysis of retinas from strain DBA/2J with elevated intraocular pressure (IOP). A model for glaucoma, DBA/2J has mutations that lead to iris atrophy and pigment dispersion. These conditions result in increased IOP. Results provide insight into the molecular changes associated with IOP elevation.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 age, 2 disease state sets

Platform:

GPL1261

Series:

GSE3554

6 Samples

Download data: CEL

(Submitter supplied) The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood contents including oxygen. We employed the single cell RNA­Seq method by performing next-generation sequencing on single glomus cell­derived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. This study has generated a CB expression profile containing novel glomus cell specific genes. more...

Organism:

Mus musculus; Mus musculus x Mus spretus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL16616 GPL9250 GPL17021

8 Samples

Download data: TXT

(Submitter supplied) C57BL/6J (B6) and DBA/2J (D2) are two of the most commonly used inbred mouse strains in neuroscience research. However, the only currently available mouse genome is based entirely on the B6 strain sequence. Subsequently, oligonucleotide microarray probes are based solely on this B6 reference sequence, making their application for gene expression profiling comparisons across mouse strains dubious due to their allelic sequence differences, including single nucleotide polymorphisms (SNPs). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL6885 GPL1261

44 Samples

Download data: CEL, TXT

(Submitter supplied) We have used commercially available oligonucleotide arrays to measure the relative expression levels of >10,000 genes and ESTs. Recent evidence suggested certain advantages of performing such expression experiments on two or more different platforms of oligonucleotide arrays. Here we have used two oligonucleotide platforms, MG U74Av2.0 arrays from Affymetrix and CodeLink UniSet1 from GE Healthcare, to assess whole brain gene expression patterns in naive C57BL/6 and DBA/2J mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2511

Platforms:

GPL81 GPL1310

27 Samples

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Comparison of whole brains from C57BL/6 and DBA/2 strains. These two strains differ in their preference for alcohol. Results provide insight into the molecular basis of this difference.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 strain sets

Platform:

GPL81

Series:

GSE2502

9 Samples

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(Submitter supplied) We hypothesize that gene expression in the aging lungs of these two strains of mice are divergent thus contributing to the disparity in the phenotypes. More specifically, (1) Aging DBA/2J mice compared to aging C57BL/6 mice are known to be accelerated in their lung physiology and morphometry; (2) C57BL/6J are known to have longer natural longevity than DBA/2J mice. In order to test these hypotheses at the gene expression level, we utilized microarray analysis to examine transcriptional differences between aging lungs of both strains of mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2929

Platform:

GPL1261

15 Samples

Download data: CEL, EXP

Analysis of lungs of C57BL/6J and DBA/2J animals at up to 26 months of age. Aging lungs of C57BL/6J and DBA/2J strains differ in physiology and morphometry. Results provide insight into the influence of genetic background on the variability of age-dependent changes in lung structure and function.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 age, 2 strain sets

Platform:

GPL1261

Series:

GSE6591

15 Samples

Download data: CEL, EXP

(Submitter supplied) The carotid body (CB), located bilaterally at the carotid artery bifurcations, is the primary sensory organ for monitoring arterial blood O2 levels. Carotid bodies are immature at birth, exhibiting low sensitivity to hypoxia and become more sensitive with maturation during the first few weeks of neonatal life. To understand the molecular basis for the postnatal developmental hypoxic responses of CB, we isolated CBs from 5-day and 21-day-old Sprague Dawley rats, and performed RNA-sequencing, which allows comprehensive analysis of gene expression. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25947

4 Samples

Download data: TSV

(Submitter supplied) Identifying sex differences in gene expression within the brain is critical for determining why multiple neurological and behavioral disorders differentially affect males and females. Several are more common or severe in males (e.g., autism and schizophrenia) or females (e.g., Alzheimer’s disease and depression). We analyzed transcriptomic data from the mouse hippocampus of six inbred strains (129S1/SvImJ, A/J, C57BL/6J, DBA/1J, DBA/2J and PWD/Ph), to provide a perspective on differences between male and female gene expression. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

36 Samples

Download data: XLSX, XML

(Submitter supplied) Age-related hearing loss (AHL) is the progressive loss of auditory function with aging. The DBA/2J (DBA) mice have been used as a model of AHL and undergoes progressive, age-related hearing loss by 12 weeks of age. Here we analyzed cochlear gene expression of 7-week-old and 36-week-old DBA mice using microarrays. Auditory brainstem response (ABR) analysis confrimed that severe age-related hearing loss occured in 36-week-old mice, whereas moderate hearing loss occured in 7-week-old mice. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3028

Platform:

GPL339

6 Samples

Download data: CEL

Analysis of cochleas of 7 and 36 week old DBA/2J animals. The DBA/2J animal suffers from age-related hearing loss (AHL), a progressive disease characterized by an age-associated loss of hair cells and spiral ganglion cells in the cochlea. Results provide insight into the molecular basis of AHL.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 age, 2 disease state sets

Platform:

GPL339

Series:

GSE6045

6 Samples

Download data: CEL

(Submitter supplied) Chronic hypoxia (CH) produces changes not fully understood in morphology and function of the carotid body (CB). To characterize the effect of CH, primary rat CB cells were exposed to 7 days of CH, total RNA was extracted, cDNA-32P synthesized and hybridized with 1185 genes printed on a nylon membrane. Out of 324 differentially expressed genes, 184 were up-regulated and 140 were down-regulated. Since data analyses showed that nitric oxide synthases (NOS) and endothelin-1 (ET-1) pathways were enriched, we studied the effect of CH at protein levels of NOS isoforms and ET-1 receptors. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL153

2 Samples

Download data: TXT

(Submitter supplied) Acute O2 sensing is a fundamental property of cells in the peripheral chemoreceptors, e.g. glomus cells in the carotid body (CB) and chromaffin cells in the adrenal medulla (AM), and is necessary for adaptation to hypoxia. The mechanisms underlying the detection of changes in O2 tension by these cells are still poorly understood. Recently, we suggested that CB glomus cells have specific metabolic features that favour their sensitivity to hypoxia (Fernandez-Aguera et al, 2015, Cell Metab.). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

11 Samples

Download data: CEL, CHP

(Submitter supplied) Investigation whether hypoxic stabilization of HIF-1alpha quantitatively or qualitatively modifies the gene expression pattern induced by poly I:C, a TLR ligand that does not induce normoxic HIF-1alpha stabilization on its own (non-HIF-1alpha-stabilizing TLR ligand). Keywords: Comparison of single and combined treatment

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

4 Samples

Download data: CEL, TXT

(Submitter supplied) We used microarrays to test the fundamental quantitative differences between central and peripheral nervous system transcriptomes. Keywords: repeat

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2209

Platform:

GPL1261

6 Samples

Download data

Analysis of spinal cord (SC) and dorsal root ganglion (DRG). The central nervous system (CNS) comprises the brain and SC; the peripheral nervous system (PNS) includes spinal and cranial nerves along with their associated DRGs. Results identify gene expression differences between the CNS and the PNS.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 tissue sets

Platform:

GPL1261

Series:

GSE5425

6 Samples

Download data

(Submitter supplied) High altitude environments are characterized by the unique and unavoidable stress of chronic hypoxia. While much is known about gene expression responses to acute or in vitro hypoxia, less is known about the gene expression profiles of animals exposed to systemic chronic hypoxia, such as that experienced at high elevations. Here we simulated the hypoxic environment of two high altitude elevations,and a third chamber recieved ambient Reno air. more...

Organism:

Mus musculus domesticus; Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4929

Platform:

GPL1261

8 Samples

Download data: CEL