GEO DataSets

Analysis of hematopoietic stem cells (HSCs), progenitor cells (HPCs), and granulocytes. Short-term (ST) and long-term (LT) repopulating HSCs examined. Results provide insight into the basis of the differences observed in cloning efficiency of each cell type in somatic cell nuclear transfer.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 development stage sets

Platform:

GPL1261

Series:

GSE5677

4 Samples

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(Submitter supplied) Since the creation of Dolly, the first sheep cloned via somatic cell nuclear transfer (SCNT), in 1997, more than a dozen species of mammals have been cloned using this technology. One hypothesis for the limited success of cloning via SCNT (1-5%) is that the clones are likely derived from adult stem cells, which form an extremely small fraction in most adult tissues. Support for this hypothesis is that the cloning efficiency of full term development using embryonic stem (ES) cells as nuclear donors is 5-10 times higher than that for somatic cells as nuclear donors. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2398

Platform:

GPL1261

4 Samples

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(Submitter supplied) Whereas cloning mammals by direct somatic cell nuclear transfer has been successful using a wide range of donor cell types, neurons from adult brain remain “unclonable” for unknown reasons. Here we examined whether neurons from adult mice could be cloned, using a combination of two epigenetic approaches. First, we used a specific antibody to discover cell types with reduced amounts of a repressive histone mark - dimethylated histone H3 lysine 9 (H3K9me2) - and identified CA1 pyramidal cells in the hippocampus and Purkinje cells in the cerebellum as candidates. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

33 Samples

Download data: TXT

(Submitter supplied) Mammalian oocytes can reprogram somatic cells into totipotent state, which allows animal cloning through somatic cell nuclear transfer (SCNT). However, the great majority of SCNT embryos fail to develop to term due to poorly defined reprogramming defects. Here we demonstrate that histone H3 lysine 9 trimethylation (H3K9me3) in donor nuclei is a major epigenetic barrier that prevents efficient nuclear reprogramming in mouse oocytes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

14 Samples

Download data: TXT

(Submitter supplied) Cloning mammals by somatic cell nuclear transfer (SCNT) is highly inefficient because of aberrant genomic reprogramming. In addition to random reprogramming errors, we hypothesized the presence of specific errors as evidenced by common anomalies among clones. We found that Xist, which normally inactivates one of the two X chromosomes in females, was ectopically expressed from the active X (Xa) chromosome in cloned mouse embryos of both sexes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

54 Samples

Download data: TXT

(Submitter supplied) DNA microarray analysis has been carried out to know the precise genome-wide gene expression profiles of somatic cloned mice derived from Sertoli or cumulus cells. It demonstrated unexpectedly large epigenetic diversity in neonatal cloned mice, despite their normal appearance and genetic identity. In three neonatal tissues of the cloned mice, a total of 9–40% of the examined genes exhibited more than two times higher or lower levels in donor cell-dependent or -independent manner compared to those in normal controls. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1943

Platform:

GPL2026

36 Samples

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Analysis of various tissues from somatically cloned neonates. Clones produced by injecting the nucleus of cumulus or Sertoli cells into enucleated mature oocytes. Results provide insight into how somatic clones differ from each other and from normal individuals produced by sexual reproduction.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 cell type, 2 other, 3 tissue sets

Platform:

GPL2026

Series:

GSE3128

36 Samples

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