GEO DataSets

Analysis of ribonucleoprotein (RNP) and polysome fractions of prepuberal and adult testes. Protein synthesis occurs on polysomes and translationally inactive mRNAs are sequestered as RNPs. Results provide insight into the translation state of individual mRNAs as spermatogenesis proceeds.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 age, 2 development stage, 2 other sets

Platform:

GPL339

Series:

GSE4711

18 Samples

Download data: CEL

(Submitter supplied) Gametes rely heavily on post-transcriptional control mechanisms to regulate their differentiation. In eggs, the storage and selective temporal activation of maternal mRNAs is essential for normal development. In the male, transcription ceases during spermiogenesis necessitating the post-transcriptional regulation of many paternal mRNAs required for spermatid differentiation and spermatozoan function. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2277

Platform:

GPL339

18 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array; Expression profiling by array

Platforms:

GPL6246 GPL16219

15 Samples

Download data: CEL, TXT

(Submitter supplied) Gene expression during spermatogenesis is highly variable and this differential pattern is very important for the successive culmination of different stages of the process, leading to production of the male gamete. Taking the time window of first wave of spermatogenesis, we did a microarray profiling of total testicular transcriptome in mouse and found several significant patterns of variable gene expression, forming upregulated and downregulated clusters among the three stages analyzed here.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

9 Samples

Download data: CEL

(Submitter supplied) Gene expression in mammalian testis undergoing spermatogenesis is under strict post transcriptional regulation and microRNAs are known to play a role in this regulation. Considering the time window of first wave of spermatogenesis, we did a microarray profiling of total testicular microRNAs in mouse and found several significant patterns of variable expression of these molecules during the different stages analyzed here.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL16219

6 Samples

Download data: TXT

(Submitter supplied) Introduction: we conduct comprehensive transcriptomic profiling analyses on three spermatogenic cell types (pachytene spermatocytes, round and elongating spermatids) purified from adult mouse testes using RNA-seq, and we determined not only the levels of both mRNAs and all known sncRNA species, but their cytoplasmic compartmentalization as well. Method: Pachytene spermatocytes, round and elongating/elongated spermatids were purified from adult mouse testes using the STA-PUT method. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11002

18 Samples

Download data: DIFF, GTF

(Submitter supplied) In obesity, adipose tissue expands by increasing the volume of existing adipocytes (hypertrophy), increasing the number of small adipocytes (hyperplasia) or both. Much is known about transcriptional control during adipogenesis, including early events. However, translational control plays a pivotal role in many dynamic processes and we presume that it is also important for the control of adipogenesis, especially in the first hours after hormonal induction. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

12 Samples

Download data: TXT

(Submitter supplied) A multitude of genes expressed solely in meiotic or postmeiotic spermatogenic cells offers a myriad of contraceptive targets. Understanding mammalian spermatozoan development and the events surrounding fertilization has grown slowly, in part because of uncertainty about the number and identity of the cellular components involved. Determination of those transcripts expressed specifically by germ cells should provide an inclusive list of probable critical proteins. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS401 GDS409 GDS410

Platforms:

GPL82 GPL83 GPL81

51 Samples

Download data: CEL

Analysis of C57BL/6 testis from day 1 to adult. Determination of transcripts expressed specifically by male germ cells late in spermatogenesis (postmeiotic) reveals many candidates for contraceptive targeting and fertilization participants.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 age, 3 tissue sets

Platform:

GPL83

Series:

GSE640

17 Samples

Download data: CEL

Analysis of C57BL/6 testis from day 1 to adult. Determination of transcripts expressed specifically by male germ cells late in spermatogenesis (postmeiotic) reveals many candidates for contraceptive targeting and fertilization participants.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 age, 3 tissue sets

Platform:

GPL82

Series:

GSE640

17 Samples

Download data: CEL

Analysis of C57BL/6 testis from day 1 to adult. Determination of transcripts expressed specifically by male germ cells late in spermatogenesis (postmeiotic) reveals many candidates for contraceptive targeting and fertilization participants.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 age, 3 tissue sets

Platform:

GPL81

Series:

GSE640

17 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae; Saccharomyces cerevisiae Sigma1278b

Type:

Expression profiling by array

Platforms:

GPL14148 GPL10045

66 Samples

Download data: GPR, TXT

(Submitter supplied) Cell survival in changing environments requires appropriate regulation of gene expression, including post-transcriptional gene regulatory mechanisms. Based on reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P-bodies and can return to active translation. We present evidence contradicting the notion that this model is a widespread and general phenomenon. more...

Organism:

Saccharomyces cerevisiae; Saccharomyces cerevisiae Sigma1278b

Type:

Expression profiling by array

Platform:

GPL14148

58 Samples

Download data: GPR

(Submitter supplied) Cell survival in changing environments requires appropriate regulation of gene expression, including post-transcriptional gene regulatory mechanisms. Based on reporter gene studies in glucose-starved yeast, it was proposed that translationally silenced eukaryotic mRNAs accumulate in P-bodies and can return to active translation. We present evidence contradicting the notion that this model is a widespread and general phenomenon. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array; Other

Platform:

GPL10045

8 Samples

Download data: TXT

(Submitter supplied) The sequence of gene regulatory events that drive neonatal germ cell development in the mammalian testis is not yet clear. We assessed changes in mRNA utilization in the neonatal testis at 1 and 4 dpp, times when the testis contains quiescent gonocytes (1 dpp) and proliferating spermatogonia (4 dpp). There are not thought to be major changes in the nature or number of somatic cells over that interval. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) Relative polysomal loading changes for wild type (N2) versus ife-1(bn127) C. elegans strains Strain ife-1(bn127) is null for the gene encoding one of five eIF4E isoforms in the worm, IFE-1. Only this isoform associates with P granules.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

12 Samples

Download data: CEL, XLS, XLSX

(Submitter supplied) Mammalian spermatogenesis is a complex and highly orchestrated combination of processes in which male germline proliferation and differentiation result in the production of mature spermatozoa. If recent genome-wide studies have contributed to the in-depth analysis of the male germline protein-coding transcriptome, little effort has yet been devoted to the systematic identification of novel unannotated transcribed regions expressed during mammalian spermatogenesis. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10287

8 Samples

Download data: BED, GTF, TXT

(Submitter supplied) Low oxygen stress dynamically regulates the translation of cellular mRNAs as a means of energy conservation in seedlings of Arabidopsis thaliana. Most of the highly hypoxia-induced mRNAs are recruited to polysomes and actively translated, whereas other cellular mRNAs become translationally inactive and are either targeted for stabilization or degradation. Here we identify the involvement of OLIGOURIDYLATE BINDING PROTEIN 1 (UBP1), a triple RNA Recognition Motif protein, in dynamic and reversible aggregation of translationally repressed mRNAs during hypoxia. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

16 Samples

Download data: CEL

(Submitter supplied) Gene expression analysis of 7d-old Arabidopsis seedlings exposed to short term (2 h) hypoxia, long term (9 h) hypoxia, and 1 h reoxygenation after long term (9 h) hypoxia to evaluate the regulation of gene expression at the level of translation. Keywords: Time Course, hypoxia recovery, polysomal mRNA, IP RNA, polysomes, hypoxia stress, reoxygenation, translational control.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

30 Samples

Download data: CEL

(Submitter supplied) Dendritic cells (DCs) are the sentinels of the mammalian immune system and they undergo a complex maturation process mediated by activation upon pathogen detection. Recent studies described the analysis of activated DCs by transcriptional profiling, but translation regulation was never taken in account. Therefore, the nature of the mRNAs being translated at various stages of DC activation was determined with the help of translational profiling, which is the sucrose gradient fractionation of polysomal-bound mRNAs combined to microarrays analysis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL, CHP