Similar studies for GEO DataSets (Select 200045982) - GEO DataSets

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Items: 20

Select item 2000459821.

EZH2 is required for germinal center formation and somatic EZH2 mutations promote lymphoid transformation

(Submitter supplied) The EZH2 histone methyltransferase is highly expressed in germinal center (GC) B-cells and targeted by somatic mutations in B-cell lymphomas. Here we find that EZH2 deletion or pharmacologic inhibition suppresses GC formation and functions in mice. EZH2 represses proliferation checkpoint genes and helps establish bivalent chromatin domains at key regulatory loci to transiently suppress GC B-cell differentiation. more...

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL13112 GPL11154 GPL10999
61 Samples

Download data: BED, TXT, WIG

Series

Accession:: GSE45982

ID:: 200045982

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000731092.

EZH2 and BCL6 cooperate to assemble CBX8-BCOR Polycomb complex to repress bivalent promoters, mediate germinal center formation and promote lymphomagenesis [RNA-seq]

(Submitter supplied) EZH2 mediates the humoral immune response and drives lymphomagenesis through de novo formation of bivalent chromatin domains and critical germinal center (GC) B cell promoters. We show that such formation is dependent on the presense of BCL6 and the presence of non-canonical PRC1-BCOR complex. We observe that BCL6 and EZH2 cooperate to accelerate diffuse large B cell lymphoma (DLBCL) development and combinatorial targeting of these repressors results in enhanced anti-lymphoma activity in vitro, in vivo, and in primary human DLBCLs.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
18 Samples

Download data: TXT

Series

Accession:: GSE73109

ID:: 200073109

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2000292823.

A Hybrid Mechanism of Action for BCL6 in B Cells Defined by Formation of Functionally Distinct Complexes at Enhancers and Promoters

(Submitter supplied) BCL6 is crucial for B-cell activation and lymphomagenesis. We used integrative genomics to explore BCL6 mechanism in normal and malignant B-cells. Surprisingly, BCL6 assembled distinct complexes at enhancers vs. promoters. At enhancers BCL6 preferentially recruited SMRT, which mediated H3K27 deacetylation through HDAC3, antagonized p300 activity and repressed transcription, but without decommissioning enhancers. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL15433 GPL10999 GPL11154
46 Samples

Download data: BED, BW, TXT, WIG

Series

Accession:: GSE29282

ID:: 200029282

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2000509124.

Genome-wide maps of chromatin state in Germinal Center B cells.

(Submitter supplied) Protection against deadly pathogens requires the production of high-affinity antibodies by B cells. High-affinity antibody-forming cells are generated in germinal centers (GC) as a result of a developmental program that is commonly altered in B cell malignancies. Identification of regulators of the GC response is crucial to develop targeted therapies for GC B cell dysfunctions including lymphomas. The Histone H3 lysine-27 methyltransferase Enhancer of Zeste homolog 2 (EZH2) is highly expressed in GC B cells and often constitutively activated in GC-derived Non-Hodgkin lymphomas (NHL), but its function in these cells remains largely unknown. more...

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL13112
3 Samples

Download data: WIG

Series

Accession:: GSE50912

ID:: 200050912

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000412395.

Gene expression profiling of two DLBCL cell lines upon shRNA mediated knockdown of EZH2

(Submitter supplied) We studied transcriptional changes by Affymetrix human microarrays in 2 DLBCL cell lines as a result of shRNA mediated knockdown of EZH2. In eukaryotes, epigenetic post-translational modification of histones is critical for regulation of chromatin structure and gene expression. EZH2 is the catalytic subunit of the Polycomb Repressive Complex 2 (PRC2) and is responsible for repressing target gene expression through methylation of histone H3 on lysine 27 (H3K27). more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
6 Samples

Download data: CEL

Series

Accession:: GSE41239

ID:: 200041239

Select item 2000409726.

EZH2 Inhibition as a Therapeutic Strategy for Lymphoma with EZH2 Activating Mutations

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by array

Platforms:: GPL570 GPL9115
52 Samples

Download data: BED, CEL

Series

Accession:: GSE40972

ID:: 200040972

Select item 2000409717.

Gene expression profiling of EZH2 mutant and wild type DLBCL cell lines treated with EZH2 inhibitor

(Submitter supplied) We studied transcriptional changes by Affymetrix human microarrays in DLBCL cell lines as a result of treatment with GSK126, a potent, highly-selective, SAM-competitive, small molecule inhibitor of EZH2 In eukaryotes, epigenetic post-translational modification of histones is critical for regulation of chromatin structure and gene expression. EZH2 is the catalytic subunit of the Polycomb Repressive Complex 2 (PRC2) and is responsible for repressing target gene expression through methylation of histone H3 on lysine 27 (H3K27). more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
40 Samples

Download data: CEL

Series

Accession:: GSE40971

ID:: 200040971

Select item 2000409708.

ChIP-seq analysis of H3K27me3 histone modification in EZH2 mutant and wild type DLBCL cell lines

(Submitter supplied) In eukaryotes, epigenetic post-translational modification of histones is critical for regulation of chromatin structure and gene expression. EZH2 is the catalytic subunit of the Polycomb Repressive Complex 2 (PRC2) and is responsible for repressing target gene expression through methylation of histone H3 on lysine 27 (H3K27). Over-expression of EZH2 is implicated in tumorigenesis and correlates with poor prognosis in multiple tumor types. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL9115
6 Samples

Download data: BED

Series

Accession:: GSE40970

ID:: 200040970

PubMed Similar studies SRA Run Selector

Select item 2000817059.

EZH2 and BCL6 cooperate to assemble CBX8-BCOR Polycomb complex to repress bivalent promoters, mediate germinal center formation and promote lymphomagenesis

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL11154
20 Samples

Download data: TXT

Series

Accession:: GSE81705

ID:: 200081705

PubMed Full text in PMC Similar studies

Select item 20008162310.

EZH2 and BCL6 cooperate to assemble CBX8-BCOR Polycomb complex to repress bivalent promoters, mediate germinal center formation and promote lymphomagenesis [ChIP-seq]

(Submitter supplied) The EZH2 histone methyltransferase mediates the humoral immune response and drives lymphomagenesis through de novo formation of bivalent chromatin domains at critical germinal center (GC) B cell promoters. Herein we show that the actions EZH2 in driving GC formation and lymphoma precursor lesions are dependent on the presence of the BCL6 transcriptional repressor, both of which are in turn dependent on the presence of non-canonical PRC1-BCOR complex. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL11154
2 Samples

Download data: TXT

Series

Accession:: GSE81623

ID:: 200081623

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20006481811.

Evolutionary analysis across mammals reveals distinct classes of long noncoding RNAs

(Submitter supplied) Recent advances in transcriptome sequencing have enabled the discovery of thousands of long non-coding RNAs (lncRNAs) across many species. Though several lncRNAs have been shown to play important roles in diverse biological processes, the functions and mechanisms of most lncRNAs remain unknown. Two significant obstacles lie between transcriptome sequencing and functional characterization of lncRNAs: identifying truly non-coding genes from de novo reconstructed transcriptomes, and prioritizing the hundreds of resulting putative lncRNAs for downstream experimental interrogation. more...

Organism:: Mus musculus castaneus; Rattus norvegicus; Homo sapiens; Mus musculus

Type:: Expression profiling by high throughput sequencing

4 related Platforms
9 Samples

Download data: TXT, WIG

Series

Accession:: GSE64818

ID:: 200064818

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20007903612.

An oncogenic Ezh2 mutation cooperates with particular genetic alterations to induce tumors in mice and redistributes H3K27m3 through the genome

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:: GPL13112
36 Samples

Download data: BW

Series

Accession:: GSE79036

ID:: 200079036

PubMed Full text in PMC Similar studies

Select item 20007903513.

An oncogenic Ezh2 mutation cooperates with particular genetic alterations to induce tumors in mice and redistributes H3K27m3 through the genome [RNA-seq]

(Submitter supplied) B-cell lymphoma and melanoma harbor recurrent mutations in the gene encoding the EZH2 histone methyltransferase, but the carcinogenic role of these mutations is unclear. Here we describe a mouse model in which the most common somatic EZH2 gain-of-function mutation (Y646F in human, Y641F in the mouse) can be conditionally expressed. Expression of Ezh2Y641F in mouse B-cells or melanocytes caused high-penetrance lymphoma or melanoma, respectively. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13112
14 Samples

Download data: TXT

Series

Accession:: GSE79035

ID:: 200079035

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20007903414.

An oncogenic Ezh2 mutation induces tumors in mice through redistribution of global H3K27 trimethylation

(Submitter supplied) B cell lymphoma and melanoma harbor recurrent mutations in the gene encoding the EZH2 histone methyltransferase (EZH2), but the carcinogenic role of these mutations is unclear. Here we describe a mouse model in which the most common somatic Ezh2 gain-of-function mutation (EZH2Y646F in human; Ezh2Y641F in mouse) is conditionally expressed. Expression of Ezh2Y641F in mouse B cells or melanocytes caused high-penetrance lymphoma or melanoma, respectively. more...

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL13112
22 Samples

Download data: BW

Series

Accession:: GSE79034

ID:: 200079034

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20017233215.

Cohesin core complex gene dosage contributes to germinal center derived lymphoma phenotypes and outcomes

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing; Other

Platforms:: GPL13112 GPL17021
23 Samples

Download data: MTX, TSV

Series

Accession:: GSE172332

ID:: 200172332

PubMed Full text in PMC Similar studies

Select item 20017233116.

Cohesin core complex gene dosage contributes to germinal center derived lymphoma phenotypes and outcomes [single-cell RNA-seq]

(Submitter supplied) The cohesin complex plays critical roles in genomic stability and gene expression through effects on 3D architecture. Cohesin core subunit genes are mutated across a wide cross-section of cancers, but not in germinal center (GC) derived lymphomas. In spite of this, haploinsufficiency of cohesin ATPase subunit Smc3 was shown to contribute to malignant transformation of GC B-cells in mice. Herein we explored potential mechanisms and clinical relevance of Smc3 deficiency in GC lymphomagenesis. Transcriptional profiling of Smc3 deficient murine lymphomas revealed downregulation of genes repressed by loss of epigenetic tumor suppressors Tet2 and Kmt2d. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
6 Samples

Download data: MTX, TSV

Series

Accession:: GSE172331

ID:: 200172331

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20017233017.

Cohesin core complex gene dosage contributes to germinal center derived lymphoma phenotypes and outcomes [bulk RNA-seq]

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13112
9 Samples

Download data: TXT

Series

Accession:: GSE172330

ID:: 200172330

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20017159818.

Cohesin core complex gene dosage contributes to germinal center derived lymphoma phenotypes and outcomes [HiC]

Organism:: Mus musculus

Type:: Other

Platform:: GPL17021
8 Samples

Download data: HIC

Series

Accession:: GSE171598

ID:: 200171598

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20014675519.

Smc3 regulates B-cell transit through germinal centers and restricts their malignant transformation (single-cell RNA-seq)

(Submitter supplied) During the germinal center (GC) reaction, B-cells undergo extensive redistribution of cohesin complex and 3D re-organization of their genomes. Yet, the significance of cohesin and architectural programming in the humoral immune response is unknown. Herein we report that conditional homozygous deletion of cohesin subunit Smc3 abrogated GC formation, yet in marked contrast, Smc3 haploinsufficiency induced GC hyperplasia, skewing of GC polarity and impaired plasma cell differentiation. more...