GEO DataSets

Analysis of CGR8 embryoid bodies differentiating for 3 or 10 days. Culturing of most embryonic stem (ES) cells require embryonic fibroblast (MEF) feeder cells which may affect their gene expression profiles. CGR8 cells are MEF-independent and represent a powerful model of ES cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 time sets

Platform:

GPL1942

Series:

GSE2469

4 Samples

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(Submitter supplied) time-course experiment with embryoid bodies of CGR8 mouse embryonic stem cells ; in the whole time-series RNA from 0 days old embryoid bodies were hybridized against RNA from 3 days and 10 days old embryoid bodies Keywords = embryoid bodies Keywords = mouse Keywords = time-course Keywords = oligonucleotide array Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1882

Platform:

GPL1942

4 Samples

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(Submitter supplied) Mouse embryonic stem (ES) cells remain pluripotent in vitro when grown in presence of Leukaemia Inhibitory Factor (LIF). LIF starvation leads to apoptosis of some of the ES-derived differentiated cells, together with p38a MAP kinase activation. Apoptosis, but not morphological cell differentiation, is blocked by a p38 inhibitor, PD 169316. To further understand the mechanism of action of this compound, we have identified its specific targets by microarray studies. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS1593 GDS1596

Platforms:

GPL81 GPL32

13 Samples

Download data: CEL, EXP

Analysis of embryonic stem cells differentiated in the presence of PD169316, an inhibitor of p38 that blocks apoptosis without altering differentiation. Results identify gene families whose transcriptional regulation correlates with cell fate: differentiation with or without apoptosis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL81

Series:

GSE2042

4 Samples

Download data: CEL, EXP

Analysis of embryonic stem cells differentiated in the presence of PD169316, an inhibitor of p38 that blocks apoptosis without altering differentiation. Results identify gene families whose transcriptional regulation correlates with cell fate: differentiation with or without apoptosis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 cell line sets

Platform:

GPL32

Series:

GSE2042

6 Samples

Download data: CEL, EXP

(Submitter supplied) The goal of this study was to identify the target genes of Stat3 involved in murine ES cell self-renewal. To achieve this goal, we used the Gs2 ES cell line expressing an inducible dominant negative mutant of Stat3 (Stat3F) in wich Y705 is mutated to phenilalanine. The Gs2 ES cells are routinely maintained in the presence of LIF 1000 U/ml and tetracycline 1µg/ml (LIF/ON). In this condition, Stat3F is not expressed and the cells are maintained in an undifferentiated phenotype. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1091 GPL1063

23 Samples

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(Submitter supplied) In order to identify the genes involved in LIF/gp130 self-renewal response, we analyzed the transcriptome of Gs2 ES cells induced to differentiate upon LIF withdrawal for 16h, 24h and 48h (LIF16, LIF24 and LIF48 respectively)and compared them to that of undifferentiated ES Gs2 cells continuously maintained in the presence of LIF. Since this cell line also carry a tetracycline regulatable dominant negative form of Stat3 (Stat3F, all the experiment was done in the presence of Tetracycline (tet ON) to avoid Stat3F expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1091

18 Samples

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(Submitter supplied) Chip contains 9216 features (probes and controls): 2014 PCR products from cDNA matrix using primers specific of genes involved in important biological process (apoptosis, cell cycle, stress response). 1684 PCR products from cDNA clones from the I.M.A.G.E consortium (Research Genetics collection). 2014 PCR products from mouse cDNA clones of a substractive bank (myodistrophic versus normal muscle). 1800 PCR products from rat cDNA clones of a substractive bank (atrophied versus normal muscle). more...

Organism:

Mus musculus

5 Series

107 Samples

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(Submitter supplied) Undifferentiated human embryonic stem cells grown on mouse embryonic fibroblast feeder layers. HES3 and HES4 are proprietary human ES cell lines of ES Cell International Pte Ltd. Singapore. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

2 Samples

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(Submitter supplied) The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic research tool for understanding the development and function of human tissues. Human ES cells can proliferate without a known limit and can form advanced derivatives of all three embryonic germ layers. What is less widely appreciated is that human ES cells can also form the extra-embryonic tissues that differentiate from the embryo before gastrulation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL2670 GPL3041 GPL3044

7 Samples

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(Submitter supplied) Transcriptional profiling of mouse embryonic stem cells comparing control untreated ES cells with ES cells transfected with a pUb-fluc-mrfp-ttk triple fusion reporter gene. The latter makes ES visualization possible by FACS and single cell microscopy, high throughput bioluminescence imaging, and PET. Goal was to determine the effects of triple-fusion (TF) gene transfection on global ES gene expression. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2187

Platform:

GPL1307

7 Samples

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Analysis of embryonic stem (ES) cells stably-transduced with a triple-fusion (TF) construct consisting of fluorescence, bioluminescence, and positron emission tomography reporter genes. Results provide insight into the feasibility of using the TF construct to track cells in vivo.

Organism:

Mus musculus

Type:

Expression profiling by array, log ratio, 2 protocol sets

Platform:

GPL1307

Series:

GSE3432

7 Samples

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(Submitter supplied) transcriptional effects of mis-expression of full length (935) myocardin in growing human skeletal myoblasts Keywords: mis-expression of transcriptional co-activator

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL97 GPL96

4 Samples

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(Submitter supplied) Normal, premalignant and various histological subtypes of testicular germ cell tumor (TGCT) tissues were hybridized against Universal Human Reference RNA (Stratagene) onto Agilent 60mer oligo microarrays (GEO accession no GPL885). In vitro time series of two TGCT cell lines, NTERA2 and 2102Ep, treated with retinoic acid for 0, 3, and 7 days were also included. The data set (30 hybridizations) is particularly useful for comparisons between various histological subtypes of TGCT versus each other or versus normal testis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL885

30 Samples

Download data: TIFF

(Submitter supplied) An 11-point time course study on differentiating embryoid bodies from a murine J1 embryonic stem cell line. The time course includes 0 hr, 6 hr, 12 hr, 18 hr, 24 hr, 36 hr, 48 hr, 4 days, 7 days, 9 days and 14 days. Keywords: Time course

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2668 GDS2669

Platforms:

GPL339 GPL340

66 Samples

Download data: CEL, EXP

(Submitter supplied) An 11-point time course study comparing V6.5 embryonic stem cells versus embryoid bodies. Time course 0 hours, 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, 48 hours, 4 days, 7 days, 9 days, and 14 days. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2671 GDS2672

Platforms:

GPL339 GPL340

66 Samples

Download data: CEL, EXP

(Submitter supplied) An 11-point time course study comparing R1 embryonic stem cells versus embryoid bodies. Time course 0 hours, 6 hours, 12 hours, 18 hours, 24 hours, 36 hours, 48 hours, 4 days, 7 days, 9 days, and 14 days. Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2666 GDS2667

Platforms:

GPL340 GPL339

66 Samples

Download data: CEL, EXP

Analysis of V6.5 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL340

Series:

GSE3231

33 Samples

Download data: CEL, EXP

Analysis of V6.5 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL339

Series:

GSE3231

33 Samples

Download data: CEL, EXP

Analysis of J1 embryonic stem cells differentiating into embryoid bodies in vitro. Cells examined at various time points up to 14 days after inducing differentiation. Results provide insight into the molecular mechanisms that drive embryonic stem cell differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL340

Series:

GSE3749

33 Samples

Download data: CEL, EXP