GEO DataSets

Analysis of embryonic day 14.5 and 18.5 kidneys from Lim1 conditional mutants. Lim1 is a homoebox gene that is essential for nephrogenesis. The mutant is ablated for Lim1 in the metanephric mesenchyme, resulting in nephron deficient kidneys. Results identify genes expressed in developing nephrons.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 development stage, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE4230

8 Samples

Download data: CEL

(Submitter supplied) BACKGROUND: Lim1 is a homeobox gene that is essential for nephrogenesis. During metanephric kidney development, Lim1 is expressed in the nephric duct, ureteric buds, and the induced metanephric mesenchyme. Conditional ablation of Lim1 in the metanephric mesenchyme blocks the formation of nephrons at the nephric vesicle stage, leading to the production of small, non-functional kidneys that lack nephrons. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1748

Platform:

GPL1261

8 Samples

Download data: CEL

(Submitter supplied) Our laboratory's interest is in understanding the molecular principles that underlie the regional organization of the mammalian metanephric kidney. Our goal is to generate a detailed spatial map of the cellular expression of selected regulatory genes during mammalian kidney development. The goal of this study is to identify a population of genes that are enriched in the renal vesicle (RV) and its derivatives using Wnt4 mutants. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

6 Samples

Download data: CEL

(Submitter supplied) The Lim1 gene has essential functions during several stages of kidney development. In particular, a tissue specific knockout in the early metanephric mesenchyme results in the formation of the earliest nephron precursor, the renal vesicle, but failure of this structure to progress to the next stage, the comma shaped body. To better understand the molecular nature of this developmental arrest we used a laser capture microdissection-microarray strategy to examine the perturbed gene expression pattern of the mutant renal vesicles. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2748

Platform:

GPL1261

9 Samples

Download data: CEL, CHP

Analysis of Lim1-null renal vesicles microdissected from embryonic day 12.5 kidneys. In Lim1 null embryos, the formation of comma shaped bodies from renal vesicles is interrupted. Results provide insight into the molecular basis of this developmental arrest.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE7257

9 Samples

Download data: CEL, CHP

(Submitter supplied) Our laboratory's interest is in understanding the molecular principles that underlie the regional organization of the mammalian metanephric kidney. Our goal is to generate a detailed spatial map of the cellular expression of selected regulatory genes during mammalian kidney development. The goal of this study is to identify a population of genes that are enriched in the renal vesicle (RV) and its derivatives using Wnt4 mutants. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL

(Submitter supplied) The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in laser capture microdissected components of the developing kidney. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3865

Platform:

GPL1261

37 Samples

Download data: CEL

Spatial and temporal analysis of laser capture microdissected wild-type, CD1 embryonic kidney sections across 3 developmental time points (E11.5, E12.5, E15.5) representing early stages of nephron formation. Results provide insight into molecular mechanisms underlying the stages of nephrogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 development stage, 9 tissue sets

Platform:

GPL1261

Series:

GSE6290

37 Samples

Download data: CEL

(Submitter supplied) E11.5 metanephric mesenchyme and ureteric bud were dissected from the E11.5 kidney rudiment using fine manual microdissection (ureteric bud only) or both fine manual microdissection and laser capture microdissection (metanephric mesenchyme) to define the gene expression profiles of these structures. Additionally, HoxA11, HoxD11 compound null E11.5 metanephric mesenchyme was obtained through laser capture microdissection allowing analysis of possible Hox targets in kidney development. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2032

Platform:

GPL1261

16 Samples

Download data: CEL

(Submitter supplied) Complete (whole) embryonic kidneys were dissected from wild type and Hoxa11, Hoxd11 compound null embryons throughout development. Targets from two biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430A and MOE430B arrays. Comparisons between normal and mutant and comparisons of development samples identified global patterns of gene regulation in kidney development Keywords: embryonic metanephric kidney, kidney development, Hoxa11, Hoxd11, compound null targeted mice

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS2030 GDS2031

Platforms:

GPL339 GPL340

28 Samples

Download data: CEL, EXP

Analysis of metanephric mesenchyme (MM) dissected from the rudimentary kidney of embryonic day E11.5 Hoxa11 Hoxd11 null mutants and wild types. Gene expression in wild type ureteric bud (UB) examined. Hox11 genes are expressed in the early MM and are required for the induction of UB formation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 tissue sets

Platform:

GPL1261

Series:

GSE3822

16 Samples

Download data: CEL

Analysis of kidneys from Hoxa11/Hoxd11 double null mutants at various embryonic ages up to E16.5. Hox11 genes play critical roles in kidney development. Results provide insight into the molecular mechanisms of Hox11 function in kidney development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 age, 2 genotype/variation sets

Platform:

GPL340

Series:

GSE3808

14 Samples

Download data: CEL, EXP

Analysis of kidneys from Hoxa11/Hoxd11 double null mutants at various embryonic ages up to E16.5. Hox11 genes play critical roles in kidney development. Results provide insight into the molecular mechanisms of Hox11 function in kidney development.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 age, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE3808

14 Samples

Download data: CEL, EXP

(Submitter supplied) Transcriptional profiling of FACS-sorted Six2-positive nephron progenitor cells from Six2CreEGFP mice without (WT) or with (MUT) homozygously floxed HDAC1 and HDAC2 alleles at the age of embryonic day 15.5. This experiment aimed to uncover the genome-wide alternation in gene expression resulting from the removal of HDAC1&2 in the nephron progenitor population and successive changes to the series of events in kidney development.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

8 Samples

Download data: TXT

(Submitter supplied) In this study we identify molecules with highly restricted expression patterns during the initial stages of metanephric development, when the ureteric bud has entered the metanephric mesenchyme and initiated branching morphogenesis. Using the Affymetrix Mouse Genome 430 2.0 Array, we compare gene expression patterns in ureteric bud tips, stalks and metanephric mesenchymes from mouse E12.5 embryos. To identify conserved molecular pathways, we also analyze transcriptional profiles in rat E13.5 ureteric buds and metanephric mesenchymes using the Affymetrix Rat Genome U34 Set. more...

Organism:

Rattus norvegicus; Mus musculus

Type:

Expression profiling by array

Datasets:

GDS1572 GDS1581 GDS1582 GDS1583

4 related Platforms

18 Samples

Download data

Analysis of ureteric bud (UB) tips and stalks and metanephric mesenchyme (MM) from E12.5 kidneys. UB-secreted factors induce conversion of adjacent MM cells into nephrons. Results identify as potential regulators a set of 20 secreted molecules specific to the UB and conserved between species.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 tissue sets

Platform:

GPL1261

Series:

GSE1983

6 Samples

Download data

Analysis of ureteric buds (UBs) and metanephric mesenchyme (MM) from E13.5 kidneys. UB-secreted factors induce conversion of adjacent MM cells into nephrons. Results identify as potential regulators a set of 20 secreted molecules specific to the UB and conserved between species.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 tissue sets

Platform:

GPL87

Series:

GSE1983

4 Samples

Download data

Analysis of ureteric buds (UBs) and metanephric mesenchyme (MM) from E13.5 kidneys. UB-secreted factors induce conversion of adjacent MM cells into nephrons. Results identify as potential regulators a set of 20 secreted molecules specific to the UB and conserved between species.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 tissue sets

Platform:

GPL86

Series:

GSE1983

4 Samples

Download data

Analysis of ureteric buds (UBs) and metanephric mesenchyme (MM) from E13.5 kidneys. UB-secreted factors induce conversion of adjacent MM cells into nephrons. Results identify as potential regulators a set of 20 secreted molecules specific to the UB and conserved between species.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 tissue sets

Platform:

GPL85

Series:

GSE1983

4 Samples

Download data

(Submitter supplied) p53 limits the self-renewing ability of a variety of stem cells. Here, contrary to its classical role in restraining cell proliferation, we demonstrate a divergent function of p53 in maintenance of self-renewal of the nephron progenitor population in the embryonic mouse kidney. p53-null nephron progenitor cells (NPC) exhibit progressive loss of the self-renewing progenitor niche in the cap mesenchyme, identified by Cited1 and Six2 expression, and loss of cap integrity. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

2 Samples

Download data: TXT