GEO DataSets

Analysis of kidney epithelial cortical collecting duct cells treated with aldosterone for 1 or 6 hours. Aldosterone stimulates Na+ transport in tight epithelia. Results identify candidate genes that regulate Na+ transport via the epithelial Na+ channel and mediate the early response to aldosterone.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 time sets

Platform:

GPL2623

Series:

GSE2933

8 Samples

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(Submitter supplied) Aldosterone is arguably the single most important hormone implicated in the control of blood pressure and extracellular fluid volume in mammals. It acts primarily by increasing the rate of transepithelial sodium transport in the kidney tubules. Several monogenetic defects resulting in hypertension have now been attributed to abnormalities in sodium handling within the aldosterone-sensitive distal nephron, where the epithelial sodium channel, ENaC, constitutes the rate-limiting step of sodium transport. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1707

Platform:

GPL2623

8 Samples

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(Submitter supplied) The Qiagen/Operon version 2.0 mouse oligonucleotide library, comprised of oligonucleotides (70-mers) representing 16,443 unique mouse genes, was printed on poly-L-lysine-coated glass slides. The library also included sets of positive and negative controls that were used for quality control purposes. Oligonucleotides were prepared in sets of 384-well plates at a concentration of 40 <E6>M. Arrays were prepared using a custom-built array printer equipped with Majer MicroQuill-2000 split tip pins with a capacity of 255 slides per print run. more...

Organism:

Mus musculus

1 DataSet

1 Series

8 Samples

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(Submitter supplied) In this study, we analyzed the transcriptome of a highly differentiated mouse clonal CCD principal cell line (mpkCCD(cl4)) and the changes in the transcriptome induced by aldosterone and vasopressin. Serial analysis of gene expression (SAGE) was performed on untreated cells and on cells treated with either aldosterone or vasopressin for 4 h. The transcriptomes in these three experimental conditions were determined by sequencing 169,721 transcript tags from the corresponding SAGE libraries. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL275

3 Samples

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(Submitter supplied) the molecular mechanisms for the biphasic effect of alcohol are not fully understood. The goal of the study is to identify genes that are differentially expressed following alcohol exposure of 50mM and 100mM ethanol for 24 hours.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

9 Samples

Download data: CEL, CHP

(Submitter supplied) Membrane-bound serine proteases are well known regulators of ENaC. More recently, they have also been identified as regulators of tight junction complexes. Here, we demonstrate that within the kidney, the serine protease Tmprss2 is co-expressed with the alpha subunit of ENaC, whereas Furin is primarily expressed in ENaC-negative cortex cells. In mCCDcl1 kidney cells, aldosterone-induced ENaC and Scnn1a-specific overexpression is accompanied by increased Tmprss2 gene expression, whereas Scnn1a silencing does not alter Tmprss2 transcription, suggesting that ENaC depends on Tmprss2 expression. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

12 Samples

Download data: COUNTS