GEO DataSets

(Submitter supplied) This series of microarrays compares gene expression by the bacterial pathogen Proteus mirabilis when the transcriptional regulator mrpJ is deleted or induced to levels found during experimental urinary tract infection. The enteric bacterium Proteus mirabilis is associated with a significant number of catheter-associated urinary tract infections. Strict regulation of the antagonistic processes of adhesion and motility, mediated by fimbriae and flagella, respectively, is essential for successful disease progression. more...

Organism:

Proteus mirabilis HI4320

Type:

Expression profiling by array

Platform:

GPL9078

8 Samples

Download data: TXT, XLSX

(Submitter supplied) The enteric bacterium Proteus mirabilis is a common cause of complicated urinary tract infections. In the study, microrarrays were used to analyze P. mirabilis gene expression in vivo from experimentally infected mice. Urine was collected at 1, 3, and 7d postinfection, and RNA was isolated from bacteria in the urine for transcriptional analysis. Across 9 microarrays, 471 genes were upregulated and 82 were downregulated in vivo compared to in vitro broth culture. more...

Organism:

Proteus mirabilis; Proteus mirabilis HI4320

Type:

Expression profiling by array

Platform:

GPL9078

9 Samples

Download data: MEV

(Submitter supplied) Proteus mirabilis is a primary cause of complicated urinary tract infections (UTI). Surprisingly, iron acquisition systems have been poorly characterized in this uropathogen despite the urinary tract being iron-limited. In this report the transcriptome of strain HI4320, cultured under iron limitation, was examined using microarray analysis. Of genes upregulated at least 2-fold, 45 were statistically significant and comprise 21 putative iron-regulated systems. more...

Organism:

Proteus mirabilis HI4320

Type:

Expression profiling by array

Platform:

GPL9078

5 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Proteus mirabilis HI4320

Type:

Expression profiling by array

Platform:

GPL9078

10 Samples

Download data: CSV

(Submitter supplied) Swarming motility by the urinary tract pathogen Proteus mirabilis has been a long-studied, but little understood phenomenon. On agar, a P. mirabilis colony grows outward in a bullseye pattern formed by consecutive waves of rapid swarming followed by consolidation into shorter cells. To examine differential gene expression in these growth phases, a microarray, constructed based on the completed genome sequence and annotation, was undertaken. more...

Organism:

Proteus mirabilis HI4320

Type:

Expression profiling by array

Platform:

GPL9078

5 Samples

Download data: CSV

(Submitter supplied) Swarming motility by the urinary tract pathogen Proteus mirabilis has been a long-studied, but little understood phenomenon. On agar, a P. mirabilis colony grows outward in a bullseye pattern formed by consecutive waves of rapid swarming followed by consolidation into shorter cells. To examine differential gene expression in these growth phases, a microarray, constructed based on the completed genome sequence and annotation, was undertaken. more...

Organism:

Proteus mirabilis HI4320

Type:

Expression profiling by array

Platform:

GPL9078

5 Samples

Download data: CSV

(Submitter supplied) 70mer oligos corresponding to 3719 predicted ORFs in the Proteus mirabilis HI4320 genome were designed by Operon Biotechnologies, Inc. The average GC% of all gene sequences was 39.618% and the Tm corresponding to this GC% was 74.0 C. Test runnings suggest that this is optimal Tm for this species. Out of 3719 sequences, 3714 have the oligos that meet the design criteria. Oligos were suspended in NaPO4 buffer and spotted in triplicate onto Corning UltraGAPS slides by Microarrays, Inc.

Organism:

Proteus mirabilis HI4320

6 Series

32 Samples

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