GEO DataSets

(Submitter supplied) E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used to assess the accuracy and precision of the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 22,000 DNA features, and was designed to detect transcripts from the entire NIA cDNA clone collection. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL870

6 Samples

Download data

(Submitter supplied) E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples at various input levels. A "full-scale" reference was prepared using the standard input level of 6.0 ug total RNA, and one round of amplification was performed with 250 ng and 50 ng total RNA inputs. For 10 ng and 2 ng inputs, two rounds of amplification were used. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL870

8 Samples

Download data

(Submitter supplied) NIA Mouse 22K Microarray v1.0 contains 21,939 60-mer oligonucleotides representing sequences derived from the NIA/NIH early mouse embryo cDNA clone set. The annotation information is based on the NIA Mouse Gene Index 4.0 http://lgsun.grc.nia.nih.gov/geneindex4, which combines genome assembly sequences from public databases (RefSeq, Ensembl, Riken), and the NIA collection of public EST libraries derived from stem cells and early stage of mouse embryos, along with human-curated genetic databases to form structure models of over 40,000 genetic loci4. more...

Organism:

Mus musculus

3 DataSets

8 Series

160 Samples

Download data