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Items: 3

1.

Transgene-free iPSCs generated from small volume peripheral blood nonmobilized CD34+ cells

(Submitter supplied) A variety of somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs), but the small number of CD34+ hematopoietic stem cells (HSCs) present in non-mobilized peripheral blood (PB) would be a convenient and desirable starting target. We report here a simple method for targeting derivation of iPSC from non-mobilized PB CD34+ HSCs using immunobead purification and 2-4 day culture to achieve enrichment of CD34+ HSCs to 80±9%, followed by reprogramming transduction with loxP-flanked polycistronic (Oct4, Klf4, Sox2, and c-Myc) STEMCCA-loxP lentivector at an MOI of 2. more...
Organism:
Homo sapiens
Type:
Methylation profiling by genome tiling array
Platform:
GPL13534
21 Samples
Download data: TXT
Series
Accession:
GSE40790
ID:
200040790

PubMed Full text in PMC Similar studies Analyze with GEO2R

2.

Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)

(Submitter supplied) 1 difference between HumanMethylation450_15017482_v.1.1.bpm and HumanMethylation450_15017482_v.1.2.bpm: 1.1: 41636384,NEGATIVE,-99,Negative 604 1.2: 41636384,RESTORATION,Green,Restore Protocol: See manufacturer's website
Organism:
Homo sapiens
1672 Series
1 Related Platform
117813 Samples
Download data: BPM, CSV, TXT, XLSX
Platform
Accession:
GPL13534
ID:
100013534

3.

A6326 PBMC

Organism:
Homo sapiens
Source name:
Uncultured peripheral blood mononuclear cells
Platform:
GPL13534
Series:
GSE40790
Download data
Sample
Accession:
GSM1139422
ID:
301139422

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db=gds|term=GSM1139422[Accession]|query=2|qty=2|blobid=MCID_66631ec0ac4a2c1050c5ce49|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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