GSM1044585[Accession] - GEO DataSets

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Items: 3

Select item 2000425361.

Transcription profiling by array of yeast single and double deletion mutants of gene-specific transcription factors

(Submitter supplied) Contains gene expression profiles of yeast single and double deletion mutants of gene-specific transcription factors. Genetic interactions were studied by comparing gene expression changes of double mutants with gene expression changes in the respective single mutants. Pairs of gene-specific transcription factors were chosen based on previous evidence for epistasis, including synthetic genetic interactions as well as common DNA binding.

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platform:: GPL11232
287 Samples

Download data: TXT

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Accession:: GSE42536

ID:: 200042536

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000112322.

A-UMCU-Y16k-1.3

(Submitter supplied) 1. Oligos are resuspended and adjusted to 10µM in 150mM phosphatebuffer, pH8.5 in Genetix 384-wells plates 2. Array production is done using a Biorobotics MicroGridII spotter located in a low dust cleanroom (ISO7/ downflow ISO5) at 18C, at 48% humidity 3. Slide postprocessing is carried out according to CodeLink protocol: Printed slides are kept at 20C, 75% humidity for 24 hours 4. Residual reactive groups are blocked using pre-warmed blocking solution (50 mM ethanolamine, 0.1 M Tris (pH 9) at 50C for 30 min; slides are rinsed thoroughly with milliQ water; slides are washed with 4x SSC, 0.1% SDS (pre-warmed to 50C) for 30 min at 50C on a shaker 5. more...