2IQJ


Conserved Protein Domain Family
ArfGap_SMAP2
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cd08859: ArfGap_SMAP2 
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Stromal membrane-associated protein 2; a subfamily of the ArfGAP family
The SMAP subfamily of Arf GTPase-activating proteins consists of the two structurally-related members, SMAP1 and SMAP2. Each SMAP member exhibits common and distinct functions in vesicle trafficking. They both bind to clathrin heavy chain molecules and are involved in the trafficking of clathrin-coated vesicles. SMAP1 preferentially exhibits GAP toward Arf6, while SMAP2 prefers Arf1 as a substrate. SMAP1 is involved in Arf6-dependent vesicle trafficking, but not Arf6-mediated actin cytoskeleton reorganization, and regulates clathrin-dependent endocytosis of the transferrin receptors and E-cadherin. SMAP2 regulates Arf1-dependent retrograde transport of TGN38/46 from the early endosome to the trans-Golgi network (TGN). SMAP2 has the Clathrin Assembly Lymphoid Myeloid (CALM)-binding domain, but SMAP1 does not.
Links
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Statistics
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PSSM-Id: 350083
Aligned: 5 rows
Threshold Bit Score: 217.547
Created: 8-Feb-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Zn binding sitearginine finger
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Structure:2IQJ; human ArfGAP domain of SMAP2 binds Zn(2+) ion
    - View structure with Cn3D
  • Comment:The coordination of the Zn2+ ion by 4 cysteines would indicate that this ion plays a structure stabilization rather than catalytical role.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                 #  #                #  #                                            
2IQJ_A     18 LANLLLEedNKFCADCQSKGPRWASWNIGVFICIRCAGIHRNLGVHISRVKSVNLDQWTQEQIQCMQEMGNGKANRLYEA 97  human
Q5F413     16 LGSLLSEeeNKYCADCQAKGPRWASWNIGVFICIRCAGIHRNLGVHISRVKSVNLDQWTQEQIQCMQEMGNGKANRLYEA 95  chicken
NP_073570  16 LANLLLEedNKFCADCQSKGPRWASWNIGVFICIRCAGIHRNLGVHISRVKSVNLDQWTQEQIQCMQEMGNGKANRLYEA 95  human
AAI06206   16 LSELLLRdeNKFCADCLAKGPRWASWNIGVFVCIRCAGVHRNLGVHISRVKSVNLDQWTQEQIQCMEEMGNGKAKRLYEA 95  African clawed frog
CAF99407   16 LNSLLALeeNKYCADCESKGPRWASWNLGIFVCIRCAGIHRNLGVHISKVKSVNLDQWTQEQVQSVQEMGNAKAKRLYEA 95  spotted green puff...

Feature 1                                
2IQJ_A     98 ylpetfrrpqidpAVEGFIRDKYEKKK 124 human
Q5F413     96 flpenfrrpqtdqAVEGFIRDKYEKKK 122 chicken
NP_073570  96 ylpetfrrpqidpAVEGFIRDKYEKKK 122 human
AAI06206   96 flpdnfirpqtdqAVEVFIREKYEKKK 122 African clawed frog
CAF99407   96 flpkcfqrpesdqSAEIFIRDKYDKKK 122 spotted green pufferfish

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