3X05,5C3V


Conserved Protein Domain Family
PIPKc
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cd00139: PIPKc 
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Phosphatidylinositol phosphate kinase (PIPK) catalytic domain family
The Phosphatidylinositol phosphate kinase (PIPK) catalytic domain family includes phosphatidylinositol 5-phosphate 4-kinases (PIP5Ks) and similar proteins. PIP5Ks catalyze the phosphorylation of phosphatidylinositol phosphate on the fourth or fifth hydroxyl of the inositol ring, to form phosphatidylinositol bisphosphate. The family includes type I and II PIP5Ks (-alpha, -beta, and -gamma) kinases. Signalling by phosphorylated species of phosphatidylinositol regulates secretion, vesicular trafficking, membrane translocation, cell adhesion, chemotaxis, DNA synthesis, and cell cycling.
Links
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Statistics
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PSSM-Id: 340436
Aligned: 108 rows
Threshold Bit Score: 157.735
Created: 1-Nov-2000
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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catalytic coreATP binding
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: catalytic core residues [active site], 3 residue positions
Conserved feature residue pattern:K D DClick to see conserved feature residue pattern help
Evidence:
  • Comment:Lys is proposed to bind the alpha-phosphate of ATP; mutation of this residue in PIPKI-alpha and PIPKII-alpha destroys enzyme activity.
  • Comment:1st Asp functions as a weak base
  • Comment:2nd Asp binds catalytic Mg2+ or Mn2+
  • Citation:PMID 9753329
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                          #             
3X05_A        68 SRFKFKEYCPMVFRNLRERFgiddqDYQNSVTrsapins-------dsqgrcgTRFLTTYdrRFVIKTVs--SEDVAEMH 138 human
5C3V_A        65 GDFKFKTYAPIAFRYFREMFgirpdDYLYSLCnepliel-------snpgasgSLFYVSSddEFIIKTVq--HKEAEFLQ 135 zebrafish
EJY78844     454 QEFRFEEYRYKDFKKIRKIAgifetDIIESFNsiqnedifqlelnkmqsgrsgKQIISTFnkRFIVKEId--SNEKNYLI 531 Oxytricha trifa...
XP_018735086 422 PDAVVQCHFPSVYENIRTLCgsyfpYYLNSFLfdhdalhe------kspgktdADFYFTAdrKYIIKTIqrkEHNTLMGE 495 Sugiyamaella li...
XP_018734322 412 GGAFIKAYSPIVYHYIRTLCgidyqEFLNSFVlhsdltkt------kspgksgSDFLFTPdgKYIIKTIkrkEHKVISNV 485 Sugiyamaella li...
CDW91623     407 FKYKIEEYQSKIFSQIRRQFgvlnnQIIYSFNpeenmeifrrvlneeqagrsgKKVFKTHdkAFILKDIn--SIEKYELL 484 Stylonychia lemnae
OMJ79731     368 LTSETIEFAPELFDFIRYISnfnnaEFAESLDplrnidslis--aneskgksgSFFMFSAdrRIVIKTIk--EKELKDLI 443 Stentor coeruleus
OMJ94713     364 QELEFIEFAGDVFQNIMNLEgispqDIIQSFDiptilsdfks-ifmnsggrsgSFFLYSSdrKFLIKTIt-dKELHLLLS 441 Stentor coeruleus
OMJ77630     343 FKCNAKIYCPLVFQHIRNLCgisndMICQSFEvvanlpslks-nagnsggrsaSFFYFSHdrRFLLKTIc-tSEKNFLLN 420 Stentor coeruleus
CDW87828     292 LSFKITEYATLIFQNLRLRQkmsqdMFRDSLLpynntktltk-sfektsakggKPLIKTHdkRFLIKEVk--KEEKDFLM 368 Stylonychia lemnae

Feature 1                                                                                        
3X05_A       139 NILKKYHQFIVECHgntlLPQFLGMYRLTvd-gvETYMVVMRNVFsh-rLTVHRKYDLKGstvareas------------ 204 human
5C3V_A       136 TLLPGYFMNLNQNMrt-lLPKFYGLYCVQad-gkNIRIVVMNNLLpr-aVPMHLKFDLKGstykrras------------ 200 zebrafish
EJY78844     532 NISNDYKNHLQENQks-lMAKIYGLFSLKienqsKVYFIIMQNLDiypeHSVIFKYDLKFseynrqhldeqiadkyrhyl 610 Oxytricha trifa...
XP_018735086 496 SFMRDYYSHIQENPdt-lIPTYLGHFTLRtl-gkDTHFVVMKNLLd---TDVNMVYDLKGikhrnrsg------------ 558 Sugiyamaella li...
XP_018734322 486 DFLGDYYNHIKAHPst-qLPFYLGNYTLLad-gkKTHFVVMKNLLq---KETELIYDLKGsshgrhag------------ 548 Sugiyamaella li...
CDW91623     485 DLAKSYSDHIFKNEdt-lLARILGMFSFKvqnqkKQYFILMQNLDylpsNKVIFRYDLKFsevnrqkivseqilntvidi 563 Stylonychia lemnae
OMJ79731     444 RILYHYARHLESNPns-lLCRMYGAFYLKfagitPMFVILMENLThn-sGTITRLYDLKGstlgrgkktlker------- 514 Stentor coeruleus
OMJ94713     442 TFLQSYHVHLYRYQds-iITRILGTFSFIvnnnyKVNFILMQSIFt--dCGIRAIYDLKGskldrmvq------------ 506 Stentor coeruleus
OMJ77630     421 ELLVDYHKFLIDHEds-iLSRIIGVFTFRfqdgtKCKVMLQSNIFp--tVPLIGVFDLKGskvdrtvsks---------- 487 Stentor coeruleus
CDW87828     369 SILPKYHLHLKRHPns-lLAKIVGIYAIRiqdkdRVYHVLMESLDpideSFIRFKYDLKFstvnrreyksrqevkvvree 447 Stylonychia lemnae

Feature 1                                                                                        
3X05_A           --------------------------------------------------------------------------------     human
5C3V_A           --------------------------------------------------------------------------------     zebrafish
EJY78844     611 mksddayndlfkqntiiesqpqiqeeeqkeivltnsqnlptqkqnkgffsrkqskqmitnqt------------------ 672 Oxytricha trifa...
XP_018735086     --------------------------------------------------------------------------------     Sugiyamaella li...
XP_018734322     --------------------------------------------------------------------------------     Sugiyamaella li...
CDW91623     564 linrhqnikeiinlksyslpqlictdgeeiemkteishvnimenfnnkadqqslddykpnqkklnifkhntlh-----ik 638 Stylonychia lemnae
OMJ79731         --------------------------------------------------------------------------------     Stentor coeruleus
OMJ94713         --------------------------------------------------------------------------------     Stentor coeruleus
OMJ77630         --------------------------------------------------------------------------------     Stentor coeruleus
CDW87828     448 llennpnleelfpkkkdltslheidkqnpglkklassngsdnrsrdtktktttksglkkpksiesgekqvnsnpygskdt 527 Stylonychia lemnae

Feature 1                                                                                        
3X05_A           --------------------------------------------------------------------------------     human
5C3V_A           --------------------------------------------------------------------------------     zebrafish
EJY78844     673 ---------sfnansirgpkqsqadsqnfqqdffemptpvpiqqshensnaklsqqfsfqtsqfagqerrqptdsnleim 743 Oxytricha trifa...
XP_018735086     --------------------------------------------------------------------------------     Sugiyamaella li...
XP_018734322     --------------------------------------------------------------------------------     Sugiyamaella li...
CDW91623     639 enslrqvffsqspqiasvskhkspvsqleslqlnssaivyshrqkdsiyssrqdanssshhqqtnninnhnssntitltl 718 Stylonychia lemnae
OMJ79731     515 -----------------------------------------------------------------------------kti 517 Stentor coeruleus
OMJ94713         --------------------------------------------------------------------------------     Stentor coeruleus
OMJ77630         --------------------------------------------------------------------------------     Stentor coeruleus
CDW87828     528 ksktntntnsnsneqdtdddesednqiladdeeidddiendekfkqldyeeqlvyreqkylykqqrmwnqdqminlkenp 607 Stylonychia lemnae

Feature 1                                                                #                       
3X05_A       205 --dkekakdlPTFKDNDFLNEgqklh----vgeesKKNFLEKLKRDVEFLAQLkimDYSLLVGIHDVdraeqeemeveer 278 human
5C3V_A       201 --pkerskgvPTYKDLDFMQDmpegil---lendhYTALSRTMQRDCRVLQSFkimDYSLLVGIHILhrageeastavpd 275 zebrafish
EJY78844     744 kkerqmfkqlKLLKDLDFKNFhnqleid-rssydnYERFIEQVHKDTLFLSKQfimDYSLLMMIVKLdsvdnmrqfkrtf 822 Oxytricha trifa...
XP_018735086 559 --vssvindlDINKDGDWLENqevil----mgkgkRDKFLIQLASDVALLEKYnimDYSLLVGIHSDpqs---------- 622 Sugiyamaella li...
XP_018734322 549 --prkdnrgrVVFKDLDWIDKneais----mtqedRDKLMVQVSKDVDLLMRHnimDYSLLVGLQTDtgt---------- 612 Sugiyamaella li...
CDW91623     719 aqenqlldalKLLKDIDFQNMhkqiilr-nksllnLQLFLQQIQRDVLYLSDQnimDYSLLLIIAKNdrkvknlirqnql 797 Stylonychia lemnae
OMJ79731     518 qeeikgnkslTPFKDLDFIRHgfkvt----lnstqYSSTIQAINRDADFLMTQgliDYSMLLLIEDSvdg---------- 583 Stentor coeruleus
OMJ94713     507 --tdeiassnIVLKDINFLIKqkkly----ltysvGQRLKEIIENDVKLFRKYeimDYSLLVAVKTSeeyskyyf----- 575 Stentor coeruleus
OMJ77630     488 srgvseiipdIIYKDLDFLSTkkkih----ienidLQRFKISILKDSKFLNDHniiDYSLLLGISDEfvsnsrpli---- 559 Stentor coeruleus
CDW87828     608 alkaqlmsklGLLKDSDFKCMhdrklpvtfddletVEEFIRIIESDVRFLEGLrimDYSLFLIVLEVpkneeqknsddpn 687 Stylonychia lemnae

Feature 1                                                                           #            
3X05_A       279 aedeecendgvggnllcsygtppdspgnllsfprffgpgefdpsvdvyamkshesspkkeVYFMAIIDILTpYDTKKKAA 358 human
5C3V_A       276 tqkkgqgqkplyctaies------------iqgesksktspqpyesmggipafnskgerlLVFIGIIDILQsYRLVKKLE 343 zebrafish
EJY78844     823 -----------------------------------------------dqvyvfqtkneqfAIVMGIIDYLQvYDFSKTIQ 855 Oxytricha trifa...
XP_018735086 623 ---------------------------------------------------------gkvRHSMGMIDSLSpYGLSKALK 645 Sugiyamaella li...
XP_018734322 613 ---------------------------------------------------------ceqRPVIGMIDTLCpFGWRKRAE 635 Sugiyamaella li...
CDW91623     798 -----------------------------------------------knrfvfvtqnknyVVLVGLIDYLQiFNFQRVIQ 830 Stylonychia lemnae
OMJ79731     584 ---------------------------------------------------------kdpVVKFGIIDFLIrYGIFKRLE 606 Stentor coeruleus
OMJ94713     576 ----------------------------------------------------ksiqkfekGYTIGLIDFLQdFSKSKKFE 603 Stentor coeruleus
OMJ77630     560 ----------------------------------------------------gygkdkglFYYVGIIDYLQtYTTFKQIE 587 Stentor coeruleus
CDW87828     688 addysiiqfvdpeqk-----------------kkeeelygrldelikfgrfvmfspskrfVYLMGLIDYLGkWNMNKRLE 750 Stylonychia lemnae

Feature 1                                            
3X05_A       359 HAAKTVKHGAga----eiSTVNPEQYSKRFNEFMSN 390 human
5C3V_A       344 HSWKALLHDGdt-----vSVHRPSFYADRFQKFMCS 374 zebrafish
EJY78844     856 QKAQRFWSVIkstkrkeiSCVQPEAYFSRFNNGIIR 891 Oxytricha trifallax
XP_018735086 646 SNFSRIVHRPss-----lNTINPRDYGKRFLNFIGS 676 Sugiyamaella lignohabitans
XP_018734322 636 NLSKSLFLGAsa-----iDVVHPEKYGTRFLNFVQS 666 Sugiyamaella lignohabitans
CDW91623     831 GQAQKIWSYLkskslkqiSCVEPIAYQERFLKGVTK 866 Stylonychia lemnae
OMJ79731     607 RLFTILLNPTkys---gaSVARPSIYAKRFKDFMTR 639 Stentor coeruleus
OMJ94713     604 GLSKKLLRMKssk---eiSSINPEDYCKRFIEFMYD 636 Stentor coeruleus
OMJ77630     588 AFSKNLIMLNvpk--ediSVTSSDLYGDRFTAFLFS 621 Stentor coeruleus
CDW87828     751 MYGKSILAHFirqn-tdfSVKPPHEFARRFLRKVKR 785 Stylonychia lemnae

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