2DEU,2HMA


Conserved Protein Domain Family
MnmA_TRMU-like
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cd01998: MnmA_TRMU-like 
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MnmA/TRMU family 2-thiouridylases and similar proteins
This family is composed of bacterial tRNA-specific 2-thiouridylase MnmA (EC 2.8.1.13) and mitochondrial tRNA-specific 2-thiouridylase 1 (TRMU or MTU1, EC 2.8.1.14). MnmA catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34. TRMU/MTU1 catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln); this is required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. This family belongs to the adenine nucleotide alpha hydrolase (AANH) superfamily that also includes other N-type ATP PPases and ATP sulfurylases. It forms an alpha/beta/alpha fold which binds to the adenosine group.
Links
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Statistics
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PSSM-Id: 467502
Aligned: 198 rows
Threshold Bit Score: 281.317
Created: 12-Dec-2003
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:2DEU: Escherichia coli tRNA-specific 2-thiouridylase MnmA binds AMP and SO4, contacts at 4A
    - View structure with Cn3D
  • Citation:PMID 8548458
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1             ### ####                     # #                                            
2DEU_A         19 KVIVGMSGGVDSSVSAWLLQQQgy----qVEGLFMKNWeedd------------------------geeYCTaaADLADA 70   Escherichia coli
EAA17181      377 KVAHMLSGGVDSLMALILLEKKkf----yIDNFYFNFTh-----------------------------yNCSk-NDLKYV 422  Plasmodium yo...
AAQ65488        2 DVAALVSGGVDSSVVVHRLCEEgykpaifYIRIGMEDKdg---------------------------yiDCPaeEDIELT 54   Porphyromonas...
XP_952380     298 RVAVLVSGGVDSSLALWIMKSRgf----dVQAFHLKVNdlsn------------------------gpgLCSs-NDISYA 348  Theileria ann...
XP_001611725  288 RVACLVSGGVDSAVALWLLKSRgf----dIEAFYLKVWsven-----------------------gdlgDCQwsADLEHA 340  Babesia bovis...
XP_002293975   12 TVAMLLSGGVDSSVAMHLLLRQny----nVTAFYLRIWledel----------------------shlgECPweDDLQVC 65   Thalassiosira...
WP_007278321    2 KIAMLLSGGVDSSVALRLLKEEgg---heITAFYLKIWledel----------------------aflgECPweEDMQYV 56   Lentisphaera ...
XP_005825870   45 EVAVLLSGGVDSSVALELVRRQgf----kPRAFYLRIWledel----------------------ahlgECPweEDWSYA 98   Guillardia th...
XP_013233686  461 EVHVLVSGGVDSAVSLLLMREWgf----rPKPIFLKVWapeaaqmkeqlqgryqqekldiaaasataaaACPwrEDAKAA 536  Eimeria tenella
OEU19414       68 TVALLLSGGVDSSVAFRLLLQQgy----dVVPFYLKIWledel----------------------ahlgECPweDDVQSC 121  Fragilariopsi...

Feature 1                                                                                         
2DEU_A         71 QAVCDKLGIELh-tVNFAAEYWDNVFELFLAEYKAGRTPNPDILCNKEIKFKaflef-------aaedLGADYIATGHYV 142  Escherichia coli
EAA17181      423 KNICKNRNNLHi--ININNEYYENVFLPMLRSYSKGQVPNPDIVCNKKIKYNfflktikkmskikgkkFNYAYISTGHYA 500  Plasmodium yo...
AAQ65488       55 TLIARRYGCPFe-vVDLHKEYWERVVSYTVETVRRGLTPNPDMMCNKLIKFGcfee---------rwgYQFDRIATGHYA 124  Porphyromonas...
XP_952380     349 MEVCNILRVTLh-vLPFSQVYDKHILSDVLDDYRKGEVPNPDILCNSRIKFGeflk--------matdWGFDYVATGHYA 419  Theileria ann...
XP_001611725  341 MGVTKLLNVPLh-vVPFQDVYHQRVLDPFVAGASLGETPNPDVCCNEFIKFGafld--------yavrWGFSHIASGHYA 411  Babesia bovis...
XP_002293975   66 QSVCEHAGNVSletVSLGKEYRERVVQYTIEEAQRGRTPNPDIMCNSRVKFGcfley------ieesgMDFDYVASGHYA 139  Thalassiosira...
WP_007278321   57 RAVCEELDIPLq-vVPLQKEYMDRVVSYVLAELKQGRTPSPDILCNERIKFGaffd---------aidETYDKVATGHYA 126  Lentisphaera ...
XP_005825870   99 SSVAEQLKVPLe-sISLQEEYWEQVVEYLIREAKMGRTPNPDIMCNSRIKFGmfee---------yvgKHFSRVASGHYA 168  Guillardia th...
XP_013233686  537 AAVAAAAGLSLe-vVPMQQQYWDRVISGFLEGARAGLTLNPDWSCNSSVKFGafae---------tldAVDYRIASGHYA 606  Eimeria tenella
OEU19414      122 RDVCDQVNIDLq-iISLQDEYHKHVMQHTLNEASLGRTPNPDILCNSRVKFGcfleyl----kglpknNQFDYIASGHYA 196  Fragilariopsi...

Feature 1                                                                                         
2DEU_A        143 Rradvd--------------------------------------gksrLLRGLDSNKDQSYFLYTLSHEQIAQSLFPVGE 184  Escherichia coli
EAA17181      501 MistnnsencnnlfnnnfpyikgknekkiknkninkikdknkirerckLVVSNDLKKDQTFFLSSFSEKQLSKFLFPLSL 580  Plasmodium yo...
AAQ65488      125 Ttdlln--------------------------------------gktyLSTAKDPVKDQTDFLAQINFAQISKLMFPIGH 166  Porphyromonas...
XP_952380     420 Tlcddfftkeqikspsy----------------ldgctsingyyrikrLCLSTDTVKDQTYFLSRLNQNQMSRLIFPIGN 483  Theileria ann...
XP_001611725  412 Siiedlvpg--------------------------------tstriarLRLCRDLRKDQTYFLSRLSQSQLRRVMFPLSQ 459  Babesia bovis...
XP_002293975  140 Qlkddddt---------------------------------ssisqkrLFRAPDPVKDQSYFLCALTQKQLSKVIFPIGE 186  Thalassiosira...
WP_007278321  127 Qvqene-------------------------------------egifeLLRAPDPVKDQTYFLSSLSQAQLSRALFPIGS 169  Lentisphaera ...
XP_005825870  169 Vschddv-----------------------------------gtrpsrLMMSPDKVKDQTYFLCNLRQDQLKHALFPIGG 213  Guillardia th...
XP_013233686  607 Rilide--------------------------------------gcprLLRGVDLTKDQSYFLSGLSSLQLSRLLFPVGA 648  Eimeria tenella
OEU19414      197 Qvkrksnnndncd------------------------enddsddgiarLYRAPDPVKDQSYFLCALDQHQLRRLLFPIGH 252  Fragilariopsi...

Feature 1                                                                                         
2DEU_A        185 LEKPQVrKIAEDLglv-takKKDSTGICFIGERKfREFLGRYLPAQ--PGKIITVD-GDEIGEHQG-----LMYHTLGQR 255  Escherichia coli
EAA17181      581 YNKNYI-KLFMKKnnitnynPNETKGLCLYGNVNmHSLLKLYFKGEeqSGEQSGEQsGEQSGEQNGeqsgkQSEEQSGKQ 659  Plasmodium yo...
AAQ65488      167 LLKSEVrAIANAAglp-sakRKDSQGICFLGKIDyNDFIERYLGKK--EGRIIELEtGKVIGRHQG-----YWFHTIGQR 238  Porphyromonas...
XP_952380     484 ILKTQVrDFAKTVglp-tynKKDSFGLCFLEDLDlSEYLTKTLGES--RGPIVEYEtNKVIGEHNG-----LYNFTIGQK 555  Theileria ann...
XP_001611725  460 LYKNEVrTIARTLgfs-nydRKESMGLCFLGKVDvKSFLSHRLGEV--PGPIVDHDtGNCIGRHHG-----LFHFTIGQR 531  Babesia bovis...
XP_002293975  187 YQKSEVrELATQFqlp-nrnRPDSQGLCFLGKVRfDDFLASYLGDR--PGDVVDALnGDVIGRHNG-----LWYHTVGQR 258  Thalassiosira...
WP_007278321  170 LMKSRVrELAEEYdlp-nkvRKDSQGICFLGKISyPDFVRFHLGEK--QGDIIEIEtDEKKGEHKG-----VWFHTIGQR 241  Lentisphaera ...
XP_005825870  214 YTKEKVrELAREFnlp-tqrRKDSQGICFLGKLKfEDFVGHHLGED--PGPVIDFHtGRELGEHRG-----LWFYTIGQR 285  Guillardia th...
XP_013233686  649 LLKTQVrKIAVSWglp-ssqRPDSQGLCFLGPLPvSQFLMHLLGEE--EGPVIHFPtGVSIGRHKG-----LWGFTVGQQ 720  Eimeria tenella
OEU19414      253 LEKTEVrQLAEDFqlp-nrnRPDSQGLCFLGKVKfRDFMQIHLGEK--FGDLVDAVtGEIIGEHHG-----VWYHTIGQR 324  Fragilariopsi...

Feature 1                                                                                         
2DEU_A        256 KGLGiggt-------------kegtEEPWYVVDKDVEN--NILVVAQgh-----eHPRLMSVGLIAQQLHWVDrepft-- 313  Escherichia coli
EAA17181      660 GEMQ---------------------SVDLYRFKKNDIKgfNLNYVNYii----diDDEIVMDKNSDIHLYTIGqnkhit- 713  Plasmodium yo...
AAQ65488      239 KGLGl-------------------sGGPWFVVKKDIKR--NIILVSRgy-----dPDAQYGKTIEMETFDFITedayeag 292  Porphyromonas...
XP_952380     556 KTINnylnp------------khvgNTPKYVVKKDIYK--NTLYVSAsynedfftNPRGIRSSFKVRDFFWNTpdykdli 621  Theileria ann...
XP_001611725  532 EGIArylns------------vrdtNQSRYVVAKDIDT--NTLYVSTryhtkcytEPGSVRRRFRISDIRWNVpdfkrf- 596  Babesia bovis...
XP_002293975  259 KGIGkvmfp------------lataKGPWYVVAKDQER--DIVYVSNryd---edDFAQARSEFEVEDVRWLAgeapmna 321  Thalassiosira...
WP_007278321  242 SGLGl-------------------sGGPWYVVKKDLAT--NIVYISHkn-----gLEDQLRDEVTVNTVHWTSgkfp--- 292  Lentisphaera ...
XP_005825870  286 KGLGsatr-------------kvthLGPWFVAGKDRER--NMLYVSNqy-----dVIEGPRRSFDVEQINWMAgslpe-- 343  Guillardia th...
XP_013233686  721 KGVVplldprlcrrchgasggppklSGPWSVVGKLPEA--NALFVVSkee---eaAAEDRLRALATEPGNYSEpl----- 790  Eimeria tenella
OEU19414      325 KGIGpylkp------------kataLGPWYVVAKDPSK--NIVYASNkyd---edVFVQSRSEFHVESLHWISgkepthl 387  Fragilariopsi...

Feature 1                                                                                         
2DEU_A        314 --------gtmrCTVKTRYRqTDIPCTVKALDDd----------------rIEVIFDEPVAAVTPGQSAVFYNg--eVCL 367  Escherichia coli
EAA17181      714 -------tyihdLYIKKFQK-KKNEKNLFSSCQwt---------------vIYKKLLEKEIKCKNGEKKIIENf---IYV 767  Plasmodium yo...
AAQ65488      293 y---wnredatpVTFKIRHTpEFTRGLLYKGEKg-----------------YRLESEERIQGIAPGQYCVIYDedhhLCY 352  Porphyromonas...
XP_952380     622 nkvqsevsdnfkFKVKLRHTpNYNYCNINFKGDeatdlqkwlpttyelaaaTIASVDNSKCSIKMIQLILTFC----TIE 697  Theileria ann...
XP_001611725  597 ------itdpnsLSMKVRHSpTLCRGDISFDDLdta-------------sgASVHLEKADIGLATGQYAVIYSg--lNCL 655  Babesia bovis...
XP_002293975  322 hh--ekswhevrLDMKIRHGpRVVQGTLMLKDDgs---------------vGNIRLDQKDGGLAPGQYVVFYQpdtlECL 384  Thalassiosira...
WP_007278321  293 --------kntnLKVKLRHGpQLVEARIMDLGEs----------------rVFIKLAEKDKGIAPGQSCIFYDd--lICL 346  Lentisphaera ...
XP_005825870  344 -------ddalrLRIKTRHGpNIHDGLLTLSQGkl---------------sGRVSLDGRDSGLAPGQWAALYDg--eECI 399  Guillardia th...
XP_013233686  791 ----------gaPLLRAAANgDKAAALALKLRQ------------------LRTCLRVDNIRWFHGKAPAGFDa--sATL 840  Eimeria tenella
OEU19414      388 l----dtngsyrLILKIRHGpKLAGGTLTVLEEe----------------dDGIDNDIEDEGLAPGQYVVFYTed-aECL 446  Fragilariopsi...

Feature 1              
2DEU_A        368 GGGII 372  Escherichia coli
EAA17181      768 TKNYS 772  Plasmodium yoelii yoelii
AAQ65488      353 GSGMI 357  Porphyromonas gingivalis W83
XP_952380     698 DVGFI 702  Theileria annulata
XP_001611725  656 GAGKI 660  Babesia bovis T2Bo
XP_002293975  385 GAAVI 389  Thalassiosira pseudonana CCMP1335
WP_007278321  347 GRGVI 351  Lentisphaera araneosa
XP_005825870  400 GGGMI 404  Guillardia theta CCMP2712
XP_013233686  841 QPGCL 845  Eimeria tenella
OEU19414      447 GGGII 451  Fragilariopsis cylindrus CCMP1102

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