CDD Conserved Protein Domain Family: RRM

cd12228: RRM_ENOX

RNA recognition motif (RRM) found in the cell surface Ecto-NOX disulfide-thiol exchanger (ECTO-NOX or ENOX) proteins

This subgroup corresponds to the conserved RNA recognition motif (RRM) in ECTO-NOX proteins (also termed ENOX), comprising a family of plant and animal NAD(P)H oxidases exhibiting both, oxidative and protein disulfide isomerase-like, activities. They are growth-related and drive cell enlargement, and may play roles in aging and neurodegenerative diseases. ENOX proteins function as terminal oxidases of plasma membrane electron transport (PMET) through catalyzing electron transport from plasma membrane quinones to extracellular oxygen, forming water as a product. They are also hydroquinone oxidases that oxidize externally supplied NADH, hence NOX. ENOX proteins harbor a di-copper center that lack flavin. ENOX proteins display protein disulfide interchange activity that is also possessed by protein disulfide isomerase. In contrast to the classic protein disulfide isomerases, ENOX proteins lack the double CXXC motif. This family includes two ENOX proteins, ENOX1 and ENOX2. ENOX1, also termed candidate growth-related and time keeping constitutive hydroquinone [NADH] oxidase (cCNOX), or cell proliferation-inducing gene 38 protein, or Constitutive Ecto-NOX (cNOX), is the constitutively expressed cell surface NADH (ubiquinone) oxidase that is ubiquitous and refractory to drugs. ENOX2, also termed APK1 antigen, or cytosolic ovarian carcinoma antigen 1, or tumor-associated hydroquinone oxidase (tNOX), is a cancer-specific variant of ENOX1 and plays a key role in cell proliferation and tumor progression. In contrast to ENOX1, ENOX2 is drug-responsive and harbors a drug binding site to which the cancer-specific S-peptide tagged pan-ENOX2 recombinant (scFv) is directed. Moreover, ENOX2 is specifically inhibited by a variety of quinone site inhibitors that have anticancer activity and is unique to the surface of cancer cells. ENOX proteins contain many functional motifs.

Q16206 122 ERPPGCKTVFVGGLPENgTEQIIVEVFE-QCGEIIAIRKSKKNFCHIRFAEEYMVDKALYLSGYRIRLGSstdk------ 194 human Q8TC92 136 ERPPGCKTVFVGGLPENaTEEIIQEVFE-QCGDITAIRKSKKNFCHIRFAEEFMVDKAIYLSGYRMRLGSstdk------ 208 human NP_001106335 69 ERPPGCKTVFVGGLPENaTDEIIQELFE-QCGDITAIRKSKKNFCHIRFAEEFMVDKAIYLSGYRMRLGSstdk------ 141 African clawed ... AAL48873 228 ERPPGCRTVFVGGLPENiTEEVIREIFE-SCGEITTLRMSKKNFCHIRYRQEGAIDRAIYLSGYRLRISAlnep------ 300 fruit fly NP_001006427 93 ERPPGCKTVFVGGLLENgTEQIIMEVFE-QCGEVIAIRESKKNFCHIRFAEEFMVDKALYLSGYRIRLGSstdk------ 165 chicken XP_002593358 39 ERPPGCRTVFVGGMPESmTEDIIQEVFE-RCGGIISIRMSKRNFCHIRFDDPGVVDRAIALSGYRIRLGQssdp------ 111 Florida lancelet EFX70988 252 ERPLGCKTVFVGGLAENiTEEILREIFE-RCGSILTIRMSKKNFAHVRFERECFVDTAILLSGYRVRLNNqkpdannpst 330 common water flea XP_002408530 86 ERPPGCRTVFVGGLPENaTEELVREVFQ-HCGTITALRMSKKNYCHIRFDTEEAVDNAIAFSGYRMKIRDqeep------ 158 black-legged tick XP_789359 190 ERPLGCKTIFVGGQPQSmTVEMMLELFTkYGGEIINIRQGKRNFCHVRFKDEQAVDKAMFVSGYRVRIGAnidp------ 263 purple urchin

Q16206 195 --kdtGRLHVDFA 205 human Q8TC92 209 --kdsGRLHVDFA 219 human NP_001106335 142 --kdsGRLHVDFA 152 African clawed frog AAL48873 301 --pnfGRLHVDYA 311 fruit fly NP_001006427 166 --kdtGRLHVDFA 176 chicken XP_002593358 112 --mnmGRLHVDYA 122 Florida lancelet EFX70988 331 sntssGRLHVDFA 343 common water flea XP_002408530 159 --sntGRLHVDFA 169 black-legged tick XP_789359 264 --pnnGRLRLDFA 274 purple urchin

Conserved Protein Domain FamilyRRM_ENOX

Conserved Protein Domain Family
RRM_ENOX