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DNA flanking Mu transposon insertions in Mu inactive lines were extracted from the UniformMu maize population by the thermo assymmetric interlaced PCR (TAIL) protocol using primers specific for the Mu terminal inverted repeat and a set of 16 arbitrary primers. Amplicons were size enriched using Sepharose 400 spin columns and cloned into the TOPO PCR4 vector.
Nucleotide
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