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1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCACTAGTTTT 3' and 5' AAAACTAGTG 3'], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.
Nucleotide
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